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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0152 [new locus tag: SA_RS00930 ]
  • pan locus tag?: SAUPAN000986000
  • symbol: capI
  • pan gene symbol?: capI
  • synonym:
  • product: capsular polysaccharide synthesis protein Cap5I

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0152 [new locus tag: SA_RS00930 ]
  • symbol: capI
  • product: capsular polysaccharide synthesis protein Cap5I
  • replicon: chromosome
  • strand: +
  • coordinates: 175291..176400
  • length: 1110
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGAATTTTAAATATTGTATCGAGTAATATTGTTCAAGACCCAAGGGTACTTAAACAA
    ATAGAAACAATTAAAGGCGTTACGAATGATTATAAAATTGTTGGAATGAATAATTCACAA
    GCTACTAATAGGCGATTGGAAAATTTAGATTGTAATTATCGTTTGTTAGGTAGCAAGGTA
    GATCCCAAAAATATTCTTTCTAAATTAATTAAGCGTATAAGATTTGCAACAGGTGTTATC
    CGAGAAATTAAAGCTTTTAAACCTGACGTGATTCATGCAAATGATTTCGACGTATTATTA
    ATGGTCTATTTAAGCAATTATAAAAAAGCTAATATTGTTTATGATGCGCATGAAATATAT
    GCGAAAAATGCCTTTATTAATAAAGTTCCACTTATTTCAAAGTTTGTAGAAAGTATAGAA
    AAACACATAGTAAAACATCGTGTTAATGCCTTCGTAACAGTAAGTCATGCAGCAAAAGAA
    TATTATCAATCTAAAGGATATAAGAAGGAAGCGAATGTTATTACGAATGCACCTATTTTA
    AATGATAGCAGAGAATTTAAAGAAATCGAAAACTTTAAAGAAATCGTATATCAAGGTCAA
    ATTGTAATGGACAGAGGATATGAAGAGTTTATTATTGCTTCATCAGCTTTTAAACAAAAT
    GCTCCTTCATTCATAATTCGAGGGTTTGGTCCGCATGAAGAAGTGATAAAAGAACTGATT
    AGTTATAACTCGGAAAATATTAGGTTGGATAAACCAGTTGAAGTAAAAGAATTGGTTGAT
    AAGTTAGCAGAAAGTAATGTTGGTGTTATCTTGACGAAACCTGTATCTATTAATTTTGAA
    TATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCT
    CCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTTTAAAGGAA
    GTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTT
    AATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAAAGT
    GAACGATTAGTAGAATTATATAAATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1110

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0152 [new locus tag: SA_RS00930 ]
  • symbol: CapI
  • description: capsular polysaccharide synthesis protein Cap5I
  • length: 369
  • theoretical pI: 9.68692
  • theoretical MW: 42595.1
  • GRAVY: -0.22981

⊟Function[edit | edit source]

  • TIGRFAM:
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 13.7)
  • TheSEED  :
    • Capsular polysaccharide synthesis enzyme CapI
    Cell Wall and Capsule Capsular and extracellular polysacchrides Serotype determining Capsular polysaccharide biosynthesis in Staphylococcus  Capsular polysaccharide synthesis enzyme Cap5I
  • PFAM:
    GT-B (CL0113) Glyco_transf_4; Glycosyltransferase Family 4 (PF13439; HMM-score: 41.2)
    and 5 more
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 25.9)
    Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 18.7)
    Glyco_transf_5; Starch synthase catalytic domain (PF08323; HMM-score: 18.1)
    Glyco_trans_4_4; Glycosyl transferase 4-like domain (PF13579; HMM-score: 17.5)
    Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 16.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.021802
    • TAT(Tat/SPI): 0.000402
    • LIPO(Sec/SPII): 0.002188
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRILNIVSSNIVQDPRVLKQIETIKGVTNDYKIVGMNNSQATNRRLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAFKPDVIHANDFDVLLMVYLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKEYYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFKQNAPSFIIRGFGPHEEVIKELISYNSENIRLDKPVEVKELVDKLAESNVGVILTKPVSINFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLRDNHDLFNHLRQNAIKASKILNWQIESERLVELYKF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Daniela Keinhörster, Shilpa Elizabeth George, Christopher Weidenmaier, Christiane Wolz
    Function and regulation of Staphylococcus aureus wall teichoic acids and capsular polysaccharides.
    Int J Med Microbiol: 2019, 309(6);151333
    [PubMed:31362856] [WorldCat.org] [DOI] (I p)
  2. ↑ S Sau, J Sun, C Y Lee
    Molecular characterization and transcriptional analysis of type 8 capsule genes in Staphylococcus aureus.
    J Bacteriol: 1997, 179(5);1614-21
    [PubMed:9045821] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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