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⊟Summary[edit | edit source]

  • pan ID?: SAUPAN000979000
  • symbol?: capE
  • synonym:
  • description?: UDP-glucose 4-epimerase

      descriptions from strain specific annotations:

    • UDP-glucose 4-epimerase
    • capsular polysaccharide biosynthesis protein Cap5E
    • capsular polysaccharide biosynthesis protein CapE
    • capsular polysaccharide synthesis enzyme CapE, putative
    • capsular polysaccharide synthesis protein Cap8E
    • NAD-dependent epimerase/dehydratase family protein
    • type 8 capsular polysaccharide synthesis protein Cap8E
    • capsular polysaccharide synthesis enzyme Cap8E
    • capsular polysaccharide synthesis enzyme CapE
    • CJ1293
    • polysaccharide biosynthesis family protein
    • polysaccharide biosynthesis protein CapD
    • UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose 3,5-epimerase
  • strand?: +
  • coordinates?: 1234504..1235622
  • synteny block?: BlockID0005130
  • occurrence?: in 100% of 34 strains

capE : capsule polysaccharide precursor UDP-N-acetylglucosamine 4,6-dehydratase/5-epimerase [1]

• A crystal structure is available : 3VVB

Staphylococci can produce up to 15 different capsules based on variants of the core trisaccharide N-acetyl-D-mannosaminouronate (ManNAcA) - N-acetyl-L-fucosamine (L-FucNAc) - N-acetyl-D-fucosamine (D-FucNAc). CapE catalyzes the first of three steps in the biosynthesis of UDP-N-acetyl-L-fucosamine (L-FucNAcA), a C-4,6 dehydrogenation and a C-5 epimerization of UDP-N-acetylglucosamine to form UDP-N-acetyl-2,6-dideoxy-beta-L-arabin-4-osamine.

Note: several strains will be capsule-deficient due to a nonfunctional CapE D75Y mutation characteristic of USA300 and USA500 lineages. They should be annotated as "nonfunctional capsule polysaccharide precursor UDP-N-acetylglucosamine 4,6-dehydratase/5-epimerase".

Historically, different capsule types were assigned their own nomenclature (i.e. Cap5E and Cap8E for the two most prevalent capsule types, CP5 and CP8). The CapE protein is 99% identical between Cap5E and Cap8E, provides the same function, and is therefore harmonized as CapE.

⊟Orthologs[edit | edit source]

    COL:
    SACOL0140 (cap5E)
    N315:
    SA0148 (capE)
    NCTC8325:
    SAOUHSC_00118
    Newman:
    NWMN_0099 (capE)
    USA300_FPR3757:
    SAUSA300_0156 (cap5E)
    04-02981:
    SA2981_0154 (capE)
    08BA02176:
    C248_0142 (capE)
    11819-97:
    MS7_0147
    6850:
    RSAU_000107 (capE)
    71193:
    ST398NM01_0167
    ECT-R 2:
    ECTR2_110
    ED133:
    SAOV_0100
    ED98:
    SAAV_0121 (cap5E)
    HO 5096 0412:
    SAEMRSA15_01190 (capE)
    JH1:
    SaurJH1_0144
    JH9:
    SaurJH9_0139
    JKD6008:
    SAA6008_00131 (cap5E)
    JKD6159:
    SAA6159_00137 (cap5E)
    JSNZ:
    JSNZ_000096 (cap8E)
    LGA251:
    SARLGA251_01270 (capE)
    M013:
    M013TW_0143
    MRSA252:
    SAR0155 (capE)
    MSHR1132:
    SAMSHR1132_01270
    MSSA476:
    SAS0128
    Mu3:
    SAHV_0152 (capE)
    Mu50:
    SAV0153 (capE)
    MW2:
    MW0128 (cap8E)
    RF122:
    SAB0094 (capE)
    ST398:
    SAPIG0167
    T0131:
    SAT0131_00143
    TCH60:
    HMPREF0772_10345 (galE)
    TW20:
    SATW20_01640 (capE)
    USA300_TCH1516:
    USA300HOU_0167 (cap5E)
    VC40:
    SAVC_00535

⊟Genome Viewer[edit | edit source]

COL
N315
NCTC8325
Newman
USA300_FPR3757

⊟Alignments[edit | edit source]

  • alignment of orthologues:
    CLUSTAL format alignment by MAFFT L-INS-i (v7.307)


    COL             MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDDIRKKYNNSKLKFYIGD
    N315            MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDDIRKKYNNSKLKFYIGD
    NCTC8325        MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDDIRKKYNNSKLKFYIGD
    Newman          MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDDIRKKYNNSKLKFYIGD
    USA300_FPR3757  MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDDIRKKYNNSKLKFYIGD
                    **********************:*************************************

    COL             VRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNIIGTENVLQSAIHQNVKKVI
    N315            VRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNIIGTENVLQSAIHQNVKKVI
    NCTC8325        VRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNIIGTENVLQSAIHQNVKKVI
    Newman          VRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNIIGTENVLQSAIHQNVKKVI
    USA300_FPR3757  VRDSQSVETAMRDVYYVFHAAALKQVPSCEFFPVEAVKTNIIGTENVLQSAIHQNVKKVI
                    ************** *********************************************

    COL             CLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLICGTRYGNVMASRGSVIPLFID
    N315            CLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLICGTRYGNVMASRGSVIPLFID
    NCTC8325        CLSTDKAAYPINARGISKAMMEKVFVAKSRNIRSEQTLICGTRYGNVMASRGSVIPLFID
    Newman          CLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLICGTRYGNVMASRGSVIPLFID
    USA300_FPR3757  CLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLICGTRYGNVMASRGSVIPLFID
                    ************* **********************************************

    COL             KIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAETGDIMVQKAPSSTVGDLATALLE
    N315            KIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAETGDIMVQKAPSSTVGDLATALLE
    NCTC8325        KIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAETGDIMVQKAPSSTVGDLATALLE
    Newman          KIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAETGDIMVQKAPSSTVGDLATALLE
    USA300_FPR3757  KIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAETGDIMVQKAPSSTVGDLATALLE
                    ************************************************************

    COL             LFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMGDYFRVPADSRDLNYSNYVETGNEK
    N315            LFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMGDYFRVPADSRDLNYSNYVETGNEK
    NCTC8325        LFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMGDYFRVPADSRDLNYSNYVETGNEK
    Newman          LFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMGDYFRVPADSRDLNYSNYVETGNEK
    USA300_FPR3757  LFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMGDYFRVPADSRDLNYSNYVETGNEK
                    ************************************************************

    COL             ITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNELNDYKASMR
    N315            ITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNELNDYKASMR
    NCTC8325        ITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNELNDYKASMR
    Newman          ITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNELNDYKASMR
    USA300_FPR3757  ITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNELNDYKASMR
                    ******************************************

  1. ↑ Takamitsu Miyafusa, Jose M M Caaveiro, Yoshikazu Tanaka, Kouhei Tsumoto
    Crystal structure of the enzyme CapF of Staphylococcus aureus reveals a unique architecture composed of two functional domains.
    Biochem J: 2012, 443(3);671-80
    [PubMed:22320426] [WorldCat.org] [DOI] (I p)    Susan Boyle-Vavra, Xue Li, Md Tauqeer Alam, Timothy D Read, Julia Sieth, Colette Cywes-Bentley, Ginette Dobbins, Michael Z David, Neha Kumar, Samantha J Eells, Loren G Miller, David J Boxrud, Henry F Chambers, Ruth Lynfield, Jean C Lee, Robert S Daum
    USA300 and USA500 clonal lineages of Staphylococcus aureus do not produce a capsular polysaccharide due to conserved mutations in the cap5 locus.
    mBio: 2015, 6(2);
    [PubMed:25852165] [WorldCat.org] [DOI] (I e)    Satsawat Visansirikul, Stephen A Kolodziej, Alexei V Demchenko
    Staphylococcus aureus capsular polysaccharides: a structural and synthetic perspective.
    Org Biomol Chem: 2020, 18(5);783-798
    [PubMed:31922180] [WorldCat.org] [DOI] (I p)
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