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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02678
  • pan locus tag?: SAUPAN005925000
  • symbol: SAOUHSC_02678
  • pan gene symbol?: narI
  • synonym:
  • product: respiratory nitrate reductase subunit gamma

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02678
  • symbol: SAOUHSC_02678
  • product: respiratory nitrate reductase subunit gamma
  • replicon: chromosome
  • strand: -
  • coordinates: 2461104..2461781
  • length: 678
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTTAATCAGTTTTTATGGGTTATATTTCCGTATTTATGTTTAGTTGTCTTTGTCGCA
    GGTCATATTGCACGTTATCGTTATGATAAATTTTCTTGGACAGCTAAATCTAGTGAATTA
    ATAGAAAGAAAAAGGTTGATGTGGGGAAGTTTATTATTTCACTTAGGTATTATTCCAGTG
    TTTTTTGGACACGTAGTTGGATTGTTAATACCGAAACCATGGATGGATGCAGTGGGTGTG
    TCTGAGCATTTATATCATATAGGCGCTGTTTATATTGGTAGCATCTTTGGTATAATAACT
    TTGATAGGGATGTTTTTATTAACAGCGAGACGTGTTACTACGAAAAGTATTCGAAGATTA
    AGTTCAGCGTCGGATATATTTGTAAATTTCTTATTATTACTTATCGTATTTATGGGATGT
    TACGCAACATTAGTAACAAATATACAACATCCAGACTTTAACTACAGAACTTCATTATCA
    ATTTGGTTTAGACAATTATTTATGTTAAAACCAGATGCGTCATTGATGAGTGGTGTGCCG
    ATTGCATTTAAAATGCATATATTGTTAGGTTTCACTATTATGGCTTGTTGGCCATTTACA
    CGTTTGGTGCATGTTTGGAGTGTACCTTTGACATATATAAATCGCAGATATATTATTTAC
    CGTAAGAATAAAGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    678

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02678
  • symbol: SAOUHSC_02678
  • description: respiratory nitrate reductase subunit gamma
  • length: 225
  • theoretical pI: 10.5772
  • theoretical MW: 26145.3
  • GRAVY: 0.651111

⊟Function[edit | edit source]

  • reaction:
    EC 1.7.99.4?  ExPASy
    Nitrate reductase Nitrite + acceptor = nitrate + reduced acceptor
  • TIGRFAM:
    Metabolism Energy metabolism Anaerobic respiratory nitrate reductase, gamma subunit (TIGR00351; EC 1.7.99.4; HMM-score: 238.5)
  • TheSEED  :
    • Respiratory nitrate reductase (quinol), gamma subunit (EC 1.7.5.1)
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Nitrate and nitrite ammonification  Respiratory nitrate reductase gamma chain (EC 1.7.99.4)
  • PFAM:
    no clan defined Nitrate_red_gam; Nitrate reductase gamma subunit (PF02665; HMM-score: 293.6)
    and 1 more
    UPF0114; Uncharacterized protein family, UPF0114 (PF03350; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013116
    • TAT(Tat/SPI): 0.000294
    • LIPO(Sec/SPII): 0.080568
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERKRLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLIGMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLSIWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYIIYRKNKV

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, NreC* (activation) regulon
    Rex*(TF)important in Energy metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    NreC*(TF)important in Nitrate and nitrite respiration; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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