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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_02221
- pan locus tag?: SAUPAN005172000
- symbol: SAOUHSC_02221
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_02221
- symbol: SAOUHSC_02221
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 2066531..2067310
- length: 780
- essential: no DEG
⊟Accession numbers[edit | edit source]
- Gene ID: 3919644 NCBI
- RefSeq: YP_500706 NCBI
- BioCyc: G1I0R-2099 BioCyc
- MicrobesOnline: 1290662 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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721ATGACTGAAAAAACTAATCAAGATGTCGATATCTTAACGCAACTAGGTGTAAAAGACATC
AGCAAACAAAATGCAAACAAGTTTTATAAATTTGCGATATACGGCAAGTTCGGGACTGGT
AAAACTACGTTTTTAACAAAAGATAACAACGCCTTAGTACTAGATATAAATGAGGACGGA
ACAACGGTAACAGAAGATGGGGCAGTTGTGCAGATTAAGAATTACAAGCATTTTAGTGCA
GTGATTAAGATGTTACCTAAAATTATTGAACAACTCAGAGAAAACGGAAAACAAATTGAT
GTTGTAGTGATTGAAACAATCCAAAAGCTACGTGATATCACTATGGACGACATCATGGAC
GGAAAATTAAAGAAACCAACATTTAATGATTGGGGCGAGTGTGCTACACGCATTGTAAGT
ATTTATCGTTATATTTCTAAATTACAAGAACATTATCAATTCCATCTTGCTATAAGTGGA
CACGAGGGAATTAACAAAGACAAAGATGATGAGGGTAGCACTATCAATCCAACAATCACG
ATAGAGGCACAAGATCAAATAAAAAAAGCGGTCATCAGTCAATCTGATGTGTTAGCAAGA
ATGACAATAGAAGAACATGAGCAAGACGGCGAAAAAGCTTATCAATATGTTCTTAACGCT
GAACCATCAAACTTATTCGAGACAAAGATAAGACACTCAAGCAACATTAAAATTAACAAC
AAACGTTTCATTAATCCAAGTATTAACGACGTAGTACAAGCAATCAGAAATGGAAACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_02221
- symbol: SAOUHSC_02221
- description: hypothetical protein
- length: 259
- theoretical pI: 6.52588
- theoretical MW: 29438.2
- GRAVY: -0.542085
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED :
- Phage phi 11 orf12 protein homolog
- PFAM: P-loop_NTPase (CL0023) AAA_24; AAA domain (PF13479; HMM-score: 109.5)and 9 moreBac_DnaA; Bacterial dnaA protein (PF00308; HMM-score: 19.6)no clan defined TFIIE-A_C; C-terminal general transcription factor TFIIE alpha (PF11521; HMM-score: 17)TAP42; TAP42-like family (PF04177; HMM-score: 15.2)SpoIISA_toxin; Toxin SpoIISA, type II toxin-antitoxin system (PF14171; HMM-score: 14.4)P-loop_NTPase (CL0023) ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 14.3)AAA_22; AAA domain (PF13401; HMM-score: 12)ABC_tran; ABC transporter (PF00005; HMM-score: 11.9)TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 11.9)cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 11.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.036041
- TAT(Tat/SPI): 0.002301
- LIPO(Sec/SPII): 0.001162
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTEKTNQDVDILTQLGVKDISKQNANKFYKFAIYGKFGTGKTTFLTKDNNALVLDINEDGTTVTEDGAVVQIKNYKHFSAVIKMLPKIIEQLRENGKQIDVVVIETIQKLRDITMDDIMDGKLKKPTFNDWGECATRIVSIYRYISKLQEHYQFHLAISGHEGINKDKDDEGSTINPTITIEAQDQIKKAVISQSDVLARMTIEEHEQDGEKAYQYVLNAEPSNLFETKIRHSSNIKINNKRFINPSINDVVQAIRNGN
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- predicted SigA promoter [3] : SAOUHSC_02219 < SAOUHSC_02220 < SAOUHSC_02221 < SAOUHSC_02222 < SAOUHSC_02223 < SAOUHSC_02224 < SAOUHSC_02225 < S859 < SAOUHSC_02227 < SAOUHSC_02228 < SAOUHSC_02229 < S861 < SAOUHSC_02233 < SAOUHSC_02234
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3] no expression profile plot available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)