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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00898
  • pan locus tag?: SAUPAN003067000
  • symbol: SAOUHSC_00898
  • pan gene symbol?: argH
  • synonym:
  • product: argininosuccinate lyase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00898
  • symbol: SAOUHSC_00898
  • product: argininosuccinate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 861935..863314
  • length: 1380
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGCAATAAAGCTTGGGGCGGTAGATTTGAAGTACAACCTGAAGAGTGGGTTGACGAC
    TTTAACGCATCCATTACTTTTGATCAAACGCTCATAGATCAAGATATCGAAGGCAGCATT
    GCACATGCAACTATGCTTGCGAATCAAGGCATTATTAGTCAACAAGACAGCGAACAAATT
    ATACAAGGACTAAAATCTATTCAACATGATTATCATCAAGATCAAATTCAATTTAGTGCA
    TCATTAGAAGATATTCATTTAAATATTGAACATGAATTAATTAAACGTATCGGTGATGCT
    GGTGGTAAGTTGCATACTGGACGCAGTAGAAACGATCAAGTTGCAACAGACATGCACTTG
    TACACTAAGAAACAAGTGCAAGATATCATCGCATTAATTAAGTCGTTACAAAGTGTAATT
    GTAGACATCGCTTCCAATAATGTTGATACAATTATGCCTGGTTATACTCATTTACAGCGT
    GCACAGCCAATTTCATTTGCACATCATATTATGACTTATTTTTGGATGTTACAACGAGAC
    CAACAACGATTTGAAGATAGTTTAAAACGAATCGATATTAATCCTTTAGGTGCAGCAGCC
    TTAAGTGGTACCACATACCCTATCGATAGACACGAGACAACAGCATTGTTGAACTTTGGC
    AGTCTCTATGAGAATAGCCTAGATGCTGTTAGTGACAGAGACTATATTATTGAAACATTG
    CATAATATTTCTTTAACGATGGTTCACTTATCACGCTTTGCAGAGGAAATTATTTTCTGG
    TCCACAGACGAAGCTAAATTCATTACATTATCAGATGCATTTTCAACTGGCTCATCTATT
    ATGCCACAAAAGAAAAATCCTGATATGGCAGAATTAATTAGAGGTAAAGTTGGTCGAACG
    ACTGGTCATTTAATGAGCATGCTTATGACTTTAAAAGGATTACCTCTAGCATATAACAAA
    GATATGCAGGAAGATAAAGAAGGTTTATTCGATGCTGTCCATACAATTAAAGGTTCTTTA
    CGTATTTTCGAAGGTATGATTCAAACGATGACAATTAATAAAGAACGACTCAATCAAACT
    GTTAAAGAAGATTTTTCAAATGCAACGGAACTAGCAGATTATTTAGTAACTAAAAATATT
    CCATTTAGAACTGCACATGAAATTGTAGGAAAAATCGTCTTAGAATGTATACAACAAGGT
    CATTATTTATTAGATGTTCCTTTAGCAACATATCAACAACATCATTCTAGTATTGATGCC
    GATATTTACGATTATTTGCAGCCTGAAAATTGTTTAAAACGACGTCAAAGTTACGGTTCA
    ACAGGTCAATCATCGGTCAAACAACAACTTGATGTTGCTAAACAATTACTATCACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00898
  • symbol: SAOUHSC_00898
  • description: argininosuccinate lyase
  • length: 459
  • theoretical pI: 5.51254
  • theoretical MW: 52016.5
  • GRAVY: -0.361438

⊟Function[edit | edit source]

  • reaction:
    EC 4.3.2.1?  ExPASy
    Argininosuccinate lyase 2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family argininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 659.5)
    and 4 more
    Metabolism Energy metabolism Other 3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 80.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis adenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 76)
    Metabolism Energy metabolism Amino acids and amines aspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 63.2)
    Metabolism Energy metabolism TCA cycle fumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 48)
  • TheSEED  :
    • Argininosuccinate lyase (EC 4.3.2.1)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Argininosuccinate lyase (EC 4.3.2.1)
  • PFAM:
    no clan defined Lyase_1; Lyase (PF00206; HMM-score: 252.1)
    and 2 more
    ASL_C2; Argininosuccinate lyase C-terminal (PF14698; HMM-score: 81.7)
    Hpt; Hpt domain (PF01627; HMM-score: 16.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00211
    • TAT(Tat/SPI): 0.00026
    • LIPO(Sec/SPII): 0.000243
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHATMLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGGKLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQRAQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLNFGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFSTGSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVHTIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKIVLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQLDVAKQLLSQ

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CodY* (repression) regulon, ArgR/AhrC* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    ArgR/AhrC*(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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