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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000923
  • pan locus tag?: SAUPAN003067000
  • symbol: argH
  • pan gene symbol?: argH
  • synonym:
  • product: argininosuccinate lyase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000923
  • symbol: argH
  • product: argininosuccinate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 920013..921392
  • length: 1380
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGCAATAAAGCTTGGGGCGGTAGATTTGAAGTACAACCTGAAGAGTGGGTTGACGAC
    TTTAACGCATCCATTACTTTTGATCAAACGCTCATAGATCAAGATATCGAAGGCAGCATT
    GCACATGCAACTATGCTTGCGAATCAAGGCATTATTAGTCAACAAGACAGCGAACAAATT
    ATACAAGGACTAAAATCTATTCAACATGATTATCATCAAGATCAAATTCAATTTAGTGCA
    TCATTAGAAGATATTCATTTAAATATTGAACATGAATTAATTAAACGTATTGGTGATGCT
    GGTGGTAAGTTGCATACTGGACGCAGTAGAAACGATCAAGTTGCAACAGACATGCACTTG
    TACACTAAGAAACAAGTGCAAGATATCATCGCATTAATTAAGTCGTTACAAAGTGTAATT
    GTAGACATCGCTTCCAATAATGTTGATACAATTATGCCTGGTTATACTCATTTACAGCGT
    GCACAGCCAATTTCATTTGCACATCATATTATGACTTATTTTTGGATGTTACAACGAGAC
    CAACAACGATTTGAAGATAGTTTAAAACGAATCGATATTAATCCTTTAGGTGCAGCAGCC
    TTAAGTGGTACCACATACCCTATCGATAGACACGAGACAACAGCATTGTTGAACTTTGGC
    AGTCTCTATGAGAATAGCCTAGATGCTGTTAGTGACAGAGACTATATTATTGAAACATTG
    CATAATATTTCTTTAACGATGGTTCACTTATCACGCTTTGCAGAGGAAATTATTTTCTGG
    TCCACAGACGAAGCTAAATTCATTACATTATCAGATGCATTTTCAACTGGCTCATCTATT
    ATGCCACAAAAGAAAAATCCTGATATGGCAGAATTAATTAGAGGTAAAGTTGGTCGAACG
    ACTGGTCATTTAATGAGCATGCTTATGACTTTAAAAGGATTACCTCTAGCATATAACAAA
    GATATGCAGGAAGATAAAGAAGGTTTATTCGATGCTGTCCATACAATTAAAGGTTCTTTA
    CGTATTTTCGAAGGTATGATTCAAACGATGACAGTTAATAAAGAACGACTCAATCAAACT
    GTTAAAGAAGATTTTTCAAATGCAACGGAACTAGCAGATTATTTAGTAACTAAAAATATT
    CCATTTAGAACTGCACATGAAATTGTAGGAAAAATCGTCTTAGAATGTATACAACAAGGT
    CATTATTTATTAGATGTTCCTTTAGCAACATATCAACAACATCATTCTAGTATTGATGCC
    GATATTTACGATTATTTGCAGCCTGAAAATTGTTTAAAACGACGTCAAAGTTACGGTTCA
    ACAGGTCAATCATCGGTCAAACAACAACTTGATGTTGCTAAACAATTACTATCACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000923
  • symbol: ArgH
  • description: argininosuccinate lyase
  • length: 459
  • theoretical pI: 5.51254
  • theoretical MW: 52002.4
  • GRAVY: -0.362092

⊟Function[edit | edit source]

  • reaction:
    EC 4.3.2.1?  ExPASy
    Argininosuccinate lyase 2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family argininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 660.1)
    and 4 more
    Metabolism Energy metabolism Other 3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 80.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis adenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 77.3)
    Metabolism Energy metabolism Amino acids and amines aspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 63.9)
    Metabolism Energy metabolism TCA cycle fumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 48.9)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined Lyase_1; Lyase (PF00206; HMM-score: 253.1)
    and 3 more
    ASL_C2; Argininosuccinate lyase C-terminal (PF14698; HMM-score: 92.3)
    Hpt; Hpt domain (PF01627; HMM-score: 17)
    TPR (CL0020) SPO22; Meiosis protein SPO22/ZIP4 like (PF08631; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9876
    • Cytoplasmic Membrane Score: 0.0017
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0106
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00211
    • TAT(Tat/SPI): 0.00026
    • LIPO(Sec/SPII): 0.000243
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHATMLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGGKLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQRAQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLNFGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFSTGSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVHTIKGSLRIFEGMIQTMTVNKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKIVLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQLDVAKQLLSQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: ArgR/AhrC (repression) regulon, CodY (repression) regulon
    ArgR/AhrC(TF)important in Arginine biosynthesis, Arginine degradation;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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