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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0963 [new locus tag: SACOL_RS04940 ]
  • pan locus tag?: SAUPAN003067000
  • symbol: argH
  • pan gene symbol?: argH
  • synonym:
  • product: argininosuccinate lyase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0963 [new locus tag: SACOL_RS04940 ]
  • symbol: argH
  • product: argininosuccinate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 965546..966925
  • length: 1380
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGCAATAAAGCTTGGGGCGGTAGATTTGAAGTACAACCTGAAGAGTGGGTTGACGAC
    TTTAACGCATCCATTACTTTTGATCAAACGCTCATAGATCAAGATATCGAAGGCAGCATT
    GCACATGCAACTATGCTTGCGAATCAAGGCATTATTAGTCAACAAGACAGCGAACAAATT
    ATACAAGGACTAAAATCTATTCAACATGATTATCATCAAGATCAAATTCAATTTAGTGCA
    TCATTAGAAGATATTCATTTAAATATTGAACATGAATTAATTAAACGTATCGGTGATGCT
    GGTGGTAAGTTGCATACTGGACGCAGTAGAAACGATCAAGTTGCAACAGACATGCACTTG
    TACACTAAGAAACAAGTGCAAGATATCATCGCATTAATTAAGTCGTTACAAAGTGTAATT
    GTAGACATCGCTTCCAATAATGTTGATACAATTATGCCTGGTTATACTCATTTACAGCGT
    GCACAGCCAATTTCATTTGCACATCATATTATGACTTATTTTTGGATGTTACAACGAGAC
    CAACAACGATTTGAAGATAGTTTAAAACGAATCGATATTAATCCTTTAGGTGCAGCAGCC
    TTAAGTGGTACCACATACCCTATCGATAGACACGAGACAACAGCATTGTTGAACTTTGGC
    AGTCTCTATGAGAATAGCCTAGATGCTGTTAGTGACAGAGACTATATTATTGAAACATTG
    CATAATATTTCTTTAACGATGGTTCACTTATCACGCTTTGCAGAGGAAATTATTTTCTGG
    TCCACAGACGAAGCTAAATTCATTACATTATCAGATGCATTTTCAACTGGCTCATCTATT
    ATGCCACAAAAGAAAAATCCTGATATGGCAGAATTAATTAGAGGTAAAGTTGGTCGAACG
    ACTGGTCATTTAATGAGCATGCTTATGACTTTAAAAGGATTACCTCTAGCATATAACAAA
    GATATGCAGGAAGATAAAGAAGGTTTATTCGATGCTGTCCATACAATTAAAGGTTCTTTA
    CGTATTTTCGAAGGTATGATTCAAACGATGACAATTAATAAAGAACGACTCAATCAAACT
    GTTAAAGAAGATTTTTCAAATGCAACGGAACTAGCAGATTATTTAGTAACTAAAAATATT
    CCATTTAGAACTGCACATGAAATTGTAGGAAAAATCGTCTTAGAATGTATACAACAAGGT
    CATTATTTATTAGATGTTCCTTTAGCAACATATCAACAACATCATTCTAGTATTGATGCC
    GATATTTACGATTATTTGCAGCCTGAAAATTGTTTAAAACGACGTCAAAGTTACGGTTCA
    ACAGGTCAATCATCGGTCAAACAACAACTTGATGTTGCTAAACAATTACTATCACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0963 [new locus tag: SACOL_RS04940 ]
  • symbol: ArgH
  • description: argininosuccinate lyase
  • length: 459
  • theoretical pI: 5.51254
  • theoretical MW: 52016.5
  • GRAVY: -0.361438

⊟Function[edit | edit source]

  • reaction:
    EC 4.3.2.1?  ExPASy
    Argininosuccinate lyase 2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family argininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 659.5)
    and 4 more
    Metabolism Energy metabolism Other 3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 80.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis adenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 76)
    Metabolism Energy metabolism Amino acids and amines aspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 63.2)
    Metabolism Energy metabolism TCA cycle fumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 48)
  • TheSEED  :
    • Argininosuccinate lyase (EC 4.3.2.1)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Argininosuccinate lyase (EC 4.3.2.1)
  • PFAM:
    no clan defined Lyase_1; Lyase (PF00206; HMM-score: 252.1)
    and 2 more
    ASL_C2; Argininosuccinate lyase C-terminal (PF14698; HMM-score: 81.7)
    Hpt; Hpt domain (PF01627; HMM-score: 16.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00211
    • TAT(Tat/SPI): 0.00026
    • LIPO(Sec/SPII): 0.000243
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHATMLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGGKLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQRAQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLNFGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFSTGSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVHTIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKIVLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQLDVAKQLLSQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: ArgR/AhrC (repression) regulon, CodY (repression) regulon
    ArgR/AhrC(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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