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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00427
  • pan locus tag?: SAUPAN002179000
  • symbol: SAOUHSC_00427
  • pan gene symbol?: sle1
  • synonym:
  • product: autolysin

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00427
  • symbol: SAOUHSC_00427
  • product: autolysin
  • replicon: chromosome
  • strand: +
  • coordinates: 427609..428613
  • length: 1005
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGCAAAAAAAAGTAATTGCAGCTATTATTGGGACAAGCGCGATTAGCGCTGTTGCGGCA
    ACTCAAGCAAATGCGGCTACAACTCACACAGTAAAACCGGGTGAATCAGTGTGGGCAATT
    TCAAATAAGTATGGGATTTCGATTGCTAAATTAAAGTCATTAAACAATTTAACATCTAAT
    CTAATTTTCCCAAACCAAGTACTAAAAGTATCTGGCTCAAGTAATTCTACGAGTAATAGT
    AGCCGTCCATCAACGAACTCAGGTGGCGGATCATACTACACAGTACAAGCAGGCGACTCA
    TTATCATTAATCGCATCAAAATATGGTACAACTTACCAAAACATTATGCGACTTAATGGT
    TTAAATAATTTCTTTATTTATCCAGGTCAAAAATTAAAAGTATCAGGTACTGCTAGCTCA
    AGTAACGCTGCGAGCAATAGTAGCCGTCCATCAACGAACTCAGGTGGCGGATCATACTAT
    ACAGTACAAGCAGGTGACTCATTGTCATTAATTGCATCAAAATATGGTACAACTTATCAA
    AAAATTATGAGCTTAAATGGCTTAAATAATTTCTTTATATATCCGGGTCAAAAATTGAAA
    GTAACTGGTAATGCATCTACGAACTCAGGATCTGCAACAACGACAAATAGAGGTTACAAT
    ACACCAGTATTCAGTCACCAAAACTTATATACATGGGGTCAATGTACATATCATGTATTT
    AATCGTCGTGCTGAAATTGGTAAAGGTATTAGTACTTATTGGTGGAATGCTAATAACTGG
    GATAACGCAGCGGCAGCAGATGGTTACACTATCGACAATAGACCTACTGTAGGTTCTATC
    GCTCAAACAGATGTAGGTTACTATGGTCATGTTATGTTTGTAGAACGTGTAAATAACGAT
    GGTAGTATTTTAGTTTCAGAAATGAACTATTCAGCTGCACCAGGTATTTTAACTTACAGA
    ACGGTACCAGCTTACCAAGTAAATAATTATAGATATATTCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1005

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00427
  • symbol: SAOUHSC_00427
  • description: autolysin
  • length: 334
  • theoretical pI: 9.98873
  • theoretical MW: 35835.5
  • GRAVY: -0.326048

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.28?  ExPASy
    N-acetylmuramoyl-L-alanine amidase Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
    EC 3.2.1.-?  ExPASy
  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination spore coat assembly protein SafA (TIGR02899; HMM-score: 73.8)
    and 1 more
    Cellular processes Cellular processes Sporulation and germination stage VI sporulation protein D (TIGR02907; HMM-score: 35.4)
  • TheSEED  :
    • N-acetylmuramoyl-L-alanine amidase Sle1 (EC 3.5.1.28)
  • PFAM:
    LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 175)
    and 13 more
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 71.5)
    LysM (CL0187) LysM3_LYK4_5; LYK4/5 LysM3 domain (PF23473; HMM-score: 56.5)
    LysM_RLK; LysM domain (PF23577; HMM-score: 26.7)
    LysM2_CERK1_LYK3_4_5; LYK3/4/5 LysM2 domain (PF23472; HMM-score: 25.8)
    LysM3_NFP; NFP LysM3 domain (PF23462; HMM-score: 23.9)
    OB (CL0021) DPOA2_OB; DNA polymerase alpha subunit B, OB domain (PF22062; HMM-score: 22.3)
    HTH (CL0123) HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 18.2)
    LysM (CL0187) NMB0315_dom_I; NMB0315-like, domain I (PF22310; HMM-score: 16.8)
    OB (CL0021) ID; Intracellular delivery domain (PF18543; HMM-score: 14.9)
    MBB (CL0193) Attacin_C; Attacin, C-terminal region (PF03769; HMM-score: 14)
    LysM (CL0187) LysM1_NFP_LYK; NFP/LYK LysM1 domain (PF23446; HMM-score: 13.6)
    Vault (CL0666) Vault_2; Major Vault Protein repeat domain (PF17794; HMM-score: 13.2)
    no clan defined SPOR; SPOR domain (PF05036; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0.0013
    • Extracellular Score: 0.9987
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: TQANAATT
  • SignalP: Signal peptide SP(Sec/SPI) length 25 aa
    • SP(Sec/SPI): 0.994915
    • TAT(Tat/SPI): 0.003012
    • LIPO(Sec/SPII): 0.001437
    • Cleavage Site: CS pos: 25-26. ANA-AT. Pr: 0.9700
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKYGISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLSLIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYYTVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRGYNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPTVGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: WalR* (activation) regulon
    WalR*(response regulator)important in cell wall homeostasis;  [4]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  4. ↑ Aurélia Delauné, Sarah Dubrac, Charlène Blanchet, Olivier Poupel, Ulrike Mäder, Aurélia Hiron, Aurélie Leduc, Catherine Fitting, Pierre Nicolas, Jean-Marc Cavaillon, Minou Adib-Conquy, Tarek Msadek
    The WalKR system controls major staphylococcal virulence genes and is involved in triggering the host inflammatory response.
    Infect Immun: 2012, 80(10);3438-53
    [PubMed:22825451] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Junko Kajimura, Tamaki Fujiwara, Sakuo Yamada, Yoshika Suzawa, Tetsuya Nishida, Yoshihiro Oyamada, Ikue Hayashi, Jun-ichi Yamagishi, Hitoshi Komatsuzawa, Motoyuki Sugai
Identification and molecular characterization of an N-acetylmuramyl-L-alanine amidase Sle1 involved in cell separation of Staphylococcus aureus.
Mol Microbiol: 2005, 58(4);1087-101
[PubMed:16262792] [WorldCat.org] [DOI] (P p)Mark J J B Sibbald, Theresa Winter, Magdalena M van der Kooi-Pol, G Buist, E Tsompanidou, Tjibbe Bosma, Tina Schäfer, Knut Ohlsen, Michael Hecker, Haike Antelmann, Susanne Engelmann, Jan Maarten van Dijl
Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus.
J Bacteriol: 2010, 192(14);3788-800
[PubMed:20472795] [WorldCat.org] [DOI] (I p)

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