PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0429 [new locus tag: NWMN_RS02425 ]
• pan locus tag^?: SAUPAN002179000
• symbol: NWMN_0429
• pan gene symbol^?: sle1
• synonym:
• product: N-acetylmuramoyl-L-alanine amidase AAA precursor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0429 [new locus tag: NWMN_RS02425 ]
• symbol: NWMN_0429
• product: N-acetylmuramoyl-L-alanine amidase AAA precursor
• replicon: chromosome
• strand: +
• coordinates: 481274..482278
• length: 1005
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330231 NCBI
• RefSeq: YP_001331463 NCBI
• BioCyc:
• MicrobesOnline: 3705960 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  GTGCAAAAAAAAGTAATTGCAGCTATTATTGGGACAAGCGCGATTAGCGCTGTTGCGGCA
  ACTCAAGCAAATGCGGCTACAACTCACACAGTAAAACCGGGTGAATCAGTGTGGGCAATT
  TCAAATAAGTATGGGATTTCGATTGCTAAATTAAAGTCATTAAACAATTTAACATCTAAT
  CTAATTTTCCCAAACCAAGTACTAAAAGTATCTGGCTCAAGTAATTCTACGAGTAATAGT
  AGCCGTCCATCAACGAACTCAGGTGGCGGATCATACTACACAGTACAAGCAGGCGACTCA
  TTATCATTAATCGCATCAAAATATGGTACAACTTACCAAAACATTATGCGACTTAATGGT
  TTAAATAATTTCTTTATTTATCCAGGTCAAAAATTAAAAGTATCAGGTACTGCTAGCTCA
  AGTAACGCTGCGAGCAATAGTAGCCGTCCATCAACGAACTCAGGTGGCGGATCATACTAT
  ACAGTACAAGCAGGTGACTCATTGTCATTAATTGCATCAAAATATGGTACAACTTATCAA
  AAAATTATGAGCTTAAATGGCTTAAATAATTTCTTTATATATCCGGGTCAAAAATTGAAA
  GTAACTGGTAATGCATCTACGAACTCAGGATCTGCAACAACGACAAATAGAGGTTACAAT
  ACACCAGTATTCAGTCACCAAAACTTATATACATGGGGTCAATGTACATATCATGTATTT
  AATCGTCGTGCTGAAATTGGTAAAGGTATTAGTACTTATTGGTGGAATGCTAATAACTGG
  GATAACGCAGCGGCAGCAGATGGTTACACTATCGACAATAGACCTACTGTAGGTTCTATC
  GCTCAAACAGATGTAGGTTACTATGGTCATGTTATGTTTGTAGAACGTGTAAATAACGAT
  GGTAGTATTTTAGTTTCAGAAATGAACTATTCAGCTGCACCAGGTATTTTAACTTACAGA
  ACGGTACCAGCTTACCAAGTAAATAATTATAGATATATTCACTAA
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⊟Protein

⊟General

• locus tag: NWMN_0429 [new locus tag: NWMN_RS02425 ]
• symbol: NWMN_0429
• description: N-acetylmuramoyl-L-alanine amidase AAA precursor
• length: 334
• theoretical pI: 9.98873
• theoretical MW: 35835.5
• GRAVY: -0.326048

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination spore coat assembly protein SafA (TIGR02899; HMM-score: 73.8)
  and 1 more
  Cellular processes Cellular processes Sporulation and germination stage VI sporulation protein D (TIGR02907; HMM-score: 35.4)
• TheSEED  :
  • N-acetylmuramoyl-L-alanine amidase Sle1 (EC 3.5.1.28)
• PFAM:
  LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 175)
  and 13 more
  Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 71.5)
  LysM (CL0187) LysM3_LYK4_5; LYK4/5 LysM3 domain (PF23473; HMM-score: 56.5)
  LysM_RLK; LysM domain (PF23577; HMM-score: 26.7)
  LysM2_CERK1_LYK3_4_5; LYK3/4/5 LysM2 domain (PF23472; HMM-score: 25.8)
  LysM3_NFP; NFP LysM3 domain (PF23462; HMM-score: 23.9)
  OB (CL0021) DPOA2_OB; DNA polymerase alpha subunit B, OB domain (PF22062; HMM-score: 22.3)
  HTH (CL0123) HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 18.2)
  LysM (CL0187) NMB0315_dom_I; NMB0315-like, domain I (PF22310; HMM-score: 16.8)
  OB (CL0021) ID; Intracellular delivery domain (PF18543; HMM-score: 14.9)
  MBB (CL0193) Attacin_C; Attacin, C-terminal region (PF03769; HMM-score: 14)
  LysM (CL0187) LysM1_NFP_LYK; NFP/LYK LysM1 domain (PF23446; HMM-score: 13.6)
  Vault (CL0666) Vault_2; Major Vault Protein repeat domain (PF17794; HMM-score: 13.2)
  no clan defined SPOR; SPOR domain (PF05036; HMM-score: 12.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cell wall & surface Score: 0.0013
  • Extracellular Score: 0.9987
• LocateP: Secretory(released) (with CS)
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -2
  • Predicted Cleavage Site: TQANAATT
• SignalP: Signal peptide SP(Sec/SPI) length 25 aa
  • SP(Sec/SPI): 0.994915
  • TAT(Tat/SPI): 0.003012
  • LIPO(Sec/SPII): 0.001437
  • Cleavage Site: CS pos: 25-26. ANA-AT. Pr: 0.9700
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220641 NCBI
• RefSeq: YP_001331463 NCBI
• UniProt: A0A0H3KAZ4 UniProt

⊟Protein sequence

• MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKYGISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLSLIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYYTVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRGYNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPTVGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• data available for NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications