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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0507 [new locus tag: SACOL_RS02560 ]
  • pan locus tag?: SAUPAN002179000
  • symbol: SACOL0507
  • pan gene symbol?: sle1
  • synonym:
  • product: LysM domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0507 [new locus tag: SACOL_RS02560 ]
  • symbol: SACOL0507
  • product: LysM domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 507935..508939
  • length: 1005
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGCAAAAAAAAGTAATTGCAGCTATTATTGGGACAAGCGCGATTAGCGCTGTTGCGGCA
    ACTCAAGCAAATGCGGCTACAACTCACACAGTAAAACCGGGTGAATCAGTGTGGGCAATT
    TCAAATAAGTATGGGATTTCGATTGCTAAATTAAAGTCATTAAACAATTTAACATCTAAT
    CTAATTTTCCCAAACCAAGTACTAAAAGTATCTGGCTCAAGTAATTCTACGAGTAATAGT
    AGCCGTCCATCAACGAACTCAGGTGGCGGATCATACTACACAGTACAAGCAGGCGACTCA
    TTATCATTAATCGCATCAAAATATGGTACAACTTACCAAAACATTATGCGACTTAATGGT
    TTAAATAATTTCTTTATTTATCCAGGTCAAAAATTAAAAGTATCAGGTACTGCTAGCTCA
    AGTAACGCTGCGAGCAATAGTAGCCGTCCATCAACGAACTCAGGTGGCGGATCATACTAT
    ACAGTACAAGCAGGTGACTCATTGTCATTAATTGCATCAAAATATGGTACAACTTATCAA
    AAAATTATGAGCTTAAATGGCTTAAATAATTTCTTTATATATCCGGGTCAAAAATTGAAA
    GTAACTGGTAATGCATCTACGAACTCAGGATCTGCAACAACGACAAATAGAGGTTACAAT
    ACACCAGTATTCAGTCACCAAAACTTATATACATGGGGTCAATGTACATATCATGTATTT
    AATCGTCGTGCTGAAATTGGTAAAGGTATTAGTACTTATTGGTGGAATGCTAATAACTGG
    GATAACGCAGCGGCAGCAGATGGTTACACTATCGACAATAGACCTACTGTAGGTTCTATC
    GCTCAAACAGATGTAGGTTACTATGGTCATGTTATGTTTGTAGAACGTGTAAATAACGAT
    GGTAGTATTTTAGTTTCAGAAATGAACTATTCAGCTGCACCAGGTATTTTAACTTACAGA
    ACGGTACCAGCTTACCAAGTAAATAATTATAGATATATTCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1005

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0507 [new locus tag: SACOL_RS02560 ]
  • symbol: SACOL0507
  • description: LysM domain-containing protein
  • length: 334
  • theoretical pI: 9.98873
  • theoretical MW: 35835.5
  • GRAVY: -0.326048

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.28?  ExPASy
    N-acetylmuramoyl-L-alanine amidase Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination spore coat assembly protein SafA (TIGR02899; HMM-score: 73.8)
    and 1 more
    Cellular processes Cellular processes Sporulation and germination stage VI sporulation protein D (TIGR02907; HMM-score: 35.4)
  • TheSEED  :
    • N-acetylmuramoyl-L-alanine amidase Sle1 (EC 3.5.1.28)
  • PFAM:
    LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 166.3)
    and 3 more
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 49.4)
    no clan defined SPOR; Sporulation related domain (PF05036; HMM-score: 13.3)
    Attacin_C; Attacin, C-terminal region (PF03769; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: TQANAATT
  • SignalP: Signal peptide SP(Sec/SPI) length 25 aa
    • SP(Sec/SPI): 0.994915
    • TAT(Tat/SPI): 0.003012
    • LIPO(Sec/SPII): 0.001437
    • Cleavage Site: CS pos: 25-26. ANA-AT. Pr: 0.9700
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKYGISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLSLIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYYTVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRGYNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPTVGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 19.08 h [4]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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