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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00093
  • pan locus tag?: SAUPAN000942000
  • symbol: SAOUHSC_00093
  • pan gene symbol?: sodM
  • synonym:
  • product: superoxide dismutase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00093
  • symbol: SAOUHSC_00093
  • product: superoxide dismutase
  • replicon: chromosome
  • strand: +
  • coordinates: 99488..100087
  • length: 600
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGCATTTAAATTACCAAATTTACCATATGCATATGATGCATTGGAACCATATATAGAT
    CAAAGAACAATGGAGTTTCATCACGACAAACATCACAATACGTACGTGACGAAATTAAAC
    GCAACAGTTGAAGGAACAGAGTTAGAGCATCAATCACTAGCGGATATGATTGCTAACTTA
    GACAAGGTACCGGAAGCGATGAGGATGTCAGTCCGTAATAATGGCGGTGGTCATTTTAAC
    CATTCATTATTCTGGGAAATACTATCACCTAATTCTGAAGAAAAAGGTGGCGTAATAGAT
    GACATCAAAGCGCAGTGGGGCACTTTAGATGAATTTAAAAATGAATTTGCAAATAAAGCA
    ACAACATTATTTGGATCAGGTTGGACTTGGTTAGTTGTTAATGATGGCAAATTAGAAATT
    GTGACAACGCCAAACCAAGATAATCCATTAACAGAAGGCAAAACACCAATCTTACTATTT
    GATGTTTGGGAGCATGCCTACTATCTGAAATATCAAAATAAACGTCCAGACTATATGACT
    GCATTTTGGAACATTGTTAACTGGAAAAAAGTTGATGAATTATACCAAGCAGCAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00093
  • symbol: SAOUHSC_00093
  • description: superoxide dismutase
  • length: 199
  • theoretical pI: 5.28358
  • theoretical MW: 23040.8
  • GRAVY: -0.583417

⊟Function[edit | edit source]

  • reaction:
    EC 1.15.1.1?  ExPASy
    Superoxide dismutase 2 superoxide + 2 H+ = O2 + H2O2
  • TIGRFAM:
  • TheSEED  :
    • Superoxide dismutase [Fe] (EC 1.15.1.1)
    • Superoxide dismutase [Mn] (EC 1.15.1.1)
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Nitric oxide synthase  Manganese superoxide dismutase (EC 1.15.1.1)
    and 5 more
    Stress Response Oxidative stress Oxidative stress  Manganese superoxide dismutase (EC 1.15.1.1)
    Stress Response Oxidative stress Oxidative stress  Superoxide dismutase [Fe] (EC 1.15.1.1)
    Stress Response Oxidative stress Oxidative stress  Superoxide dismutase [Mn] (EC 1.15.1.1)
    Stress Response Oxidative stress Protection from Reactive Oxygen Species  Manganese superoxide dismutase (EC 1.15.1.1)
    Stress Response Oxidative stress Protection from Reactive Oxygen Species  Superoxide dismutase [Fe] (EC 1.15.1.1)
  • PFAM:
    no clan defined Sod_Fe_C; Iron/manganese superoxide dismutases, C-terminal domain (PF02777; HMM-score: 135.5)
    Sod_Fe_N; Iron/manganese superoxide dismutases, alpha-hairpin domain (PF00081; HMM-score: 113.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Fe2+, Mn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002595
    • TAT(Tat/SPI): 0.000228
    • LIPO(Sec/SPII): 0.00037
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVEGTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDIKAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLFDVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

M W Valderas, M E Hart
Identification and characterization of a second superoxide dismutase gene (sodM) from Staphylococcus aureus.
J Bacteriol: 2001, 183(11);3399-407
[PubMed:11344148] [WorldCat.org] [DOI] (P p)Michelle Wright Valderas, Joshua W Gatson, Natalie Wreyford, Mark E Hart
The superoxide dismutase gene sodM is unique to Staphylococcus aureus: absence of sodM in coagulase-negative staphylococci.
J Bacteriol: 2002, 184(9);2465-72
[PubMed:11948161] [WorldCat.org] [DOI] (P p)Michail H Karavolos, Malcolm J Horsburgh, Eileen Ingham, Simon J Foster
Role and regulation of the superoxide dismutases of Staphylococcus aureus.
Microbiology (Reading): 2003, 149(Pt 10);2749-2758
[PubMed:14523108] [WorldCat.org] [DOI] (P p)C Poyart, P Berche, P Trieu-Cuot
Characterization of superoxide dismutase genes from gram-positive bacteria by polymerase chain reaction using degenerate primers.
FEMS Microbiol Lett: 1995, 131(1);41-5
[PubMed:7557308] [WorldCat.org] [DOI] (P p)

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