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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0128 [new locus tag: SA_RS00805 ]
  • pan locus tag?: SAUPAN000942000
  • symbol: sodM
  • pan gene symbol?: sodM
  • synonym:
  • product: superoxide dismutase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0128 [new locus tag: SA_RS00805 ]
  • symbol: sodM
  • product: superoxide dismutase
  • replicon: chromosome
  • strand: +
  • coordinates: 148053..148652
  • length: 600
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGCATTTAAATTACCAAATTTACCATATGCATATGATGCATTGGAACCATATATAGAT
    CAAAGAACAATGGAGTTTCATCACGACAAACATCACAATACGTACGTGACGAAATTAAAC
    GCAACAGTTGAAGGAACAGAGTTAGAGCATCAATCACTAGCGGATATGATTGCTAACTTA
    GACAAGGTACCGGAAGCGATGAGGATGTCAGTCCGTAATAATGGCGGTGGTCATTTTAAC
    CATTCATTATTCTGGGAAATACTATCACCTAATTCTGAAGAAAAAGGTGGCGTAATAGAT
    GACATCAAAGCGCAGTGGGGCACTTTAGATGAATTTAAAAATGAATTTGCAAATAAAGCA
    ACAACATTATTTGGATCAGGTTGGACTTGGTTAGTTGTTAATGATGGAAAATTAGAAATT
    GTGACAACGCCAAACCAAGATAATCCATTAACAGAAGGCAAAACACCAATCTTACTATTT
    GATGTTTGGGAGCATGCCTACTATCTGAAATATCAAAATAAACGTCCAGACTATATGACT
    GCATTTTGGAATATTGTTAACTGGAAAAAAGTTGATGAATTATACCAAGCAGCAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0128 [new locus tag: SA_RS00805 ]
  • symbol: SodM
  • description: superoxide dismutase
  • length: 199
  • theoretical pI: 5.28358
  • theoretical MW: 23040.8
  • GRAVY: -0.583417

⊟Function[edit | edit source]

  • reaction:
    EC 1.15.1.1?  ExPASy
    Superoxide dismutase 2 superoxide + 2 H+ = O2 + H2O2
  • TIGRFAM:
  • TheSEED  :
    • Superoxide dismutase [Fe] (EC 1.15.1.1)
    • Superoxide dismutase [Mn] (EC 1.15.1.1)
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Nitric oxide synthase  Manganese superoxide dismutase (EC 1.15.1.1)
    and 5 more
    Stress Response Oxidative stress Oxidative stress  Manganese superoxide dismutase (EC 1.15.1.1)
    Stress Response Oxidative stress Oxidative stress  Superoxide dismutase [Fe] (EC 1.15.1.1)
    Stress Response Oxidative stress Oxidative stress  Superoxide dismutase [Mn] (EC 1.15.1.1)
    Stress Response Oxidative stress Protection from Reactive Oxygen Species  Manganese superoxide dismutase (EC 1.15.1.1)
    Stress Response Oxidative stress Protection from Reactive Oxygen Species  Superoxide dismutase [Fe] (EC 1.15.1.1)
  • PFAM:
    no clan defined Sod_Fe_C; Iron/manganese superoxide dismutases, C-terminal domain (PF02777; HMM-score: 135.5)
    Sod_Fe_N; Iron/manganese superoxide dismutases, alpha-hairpin domain (PF00081; HMM-score: 113.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Fe2+, Mn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002595
    • TAT(Tat/SPI): 0.000228
    • LIPO(Sec/SPII): 0.00037
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVEGTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDIKAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLFDVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Michelle Wright Valderas, Joshua W Gatson, Natalie Wreyford, Mark E Hart
The superoxide dismutase gene sodM is unique to Staphylococcus aureus: absence of sodM in coagulase-negative staphylococci.
J Bacteriol: 2002, 184(9);2465-72
[PubMed:11948161] [WorldCat.org] [DOI] (P p)Michail H Karavolos, Malcolm J Horsburgh, Eileen Ingham, Simon J Foster
Role and regulation of the superoxide dismutases of Staphylococcus aureus.
Microbiology (Reading): 2003, 149(Pt 10);2749-2758
[PubMed:14523108] [WorldCat.org] [DOI] (P p)

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