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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07710 [old locus tag: SACOL1514 ]
  • pan locus tag?: SAUPAN003935000
  • symbol: SACOL_RS07710
  • pan gene symbol?: gpsA
  • synonym:
  • product: glycerol-3-phosphate dehydrogenase (NAD(P)(+))

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS07710 [old locus tag: SACOL1514 ]
  • symbol: SACOL_RS07710
  • product: glycerol-3-phosphate dehydrogenase (NAD(P)(+))
  • replicon: chromosome
  • strand: -
  • coordinates: 1552712..1553710
  • length: 999
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1552712..1553710) NCBI
  • BioCyc: SACOL_RS07710 BioCyc
  • MicrobesOnline: see SACOL1514

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACTAAAATTACCGTTTTTGGTATGGGAAGTTTTGGGACAGCCCTTGCCAATGTTCTT
    GCAGAAAATGGACATGATGTTTTGATGTGGGGTAAAAATCAAGATGCTGTTGATGAATTA
    AATACATGTCATACAAATAAAAAGTATTTAAAATACGCGAAATTAGATGTTAACATCATC
    GCTACTTCAGATATGACCAAAGCAATTCAATTTGCAGATATTTACTTAATGGCTTTACCT
    ACTAAAGCAATGCGAGAAGTTGCTTCTCAAATTAATGATAAGCTGACCTCTAAAAAGACT
    TTTATACATGTTGCTAAAGGTATTGAAAATGGGACGTTTAAACGTGTGTCAGAAATGATT
    GAAGATTCTATTTCACCTGAATATAATGCAGGTATTGGCGTGTTGTCAGGGCCAAGTCAT
    GCGGAAGAAGTTGTAGTCAAGCAACCAACTACAGTTGCTGCTTCATCAAAAGATAAAAGT
    GTAAGTAAATTAACGCAAGATTTATTTATGAATGATTATTTGCGTGTGTACACGAATGAT
    GACTTGATTGGTGTTGAACTTGGTGGTGCATTGAAAAATATCATCGCAGTAGCAAGTGGT
    ATCGTAGCTGGAATTGGCTACGGTGATAATGCAAAAGCTGCATTAATGACTCGTGGCTTA
    GCGGAAATTAGTAGATTAGGTGAAAAGTTAGGTGCCGATCCTATGACATTTCTAGGTTTA
    GGTGGTATCGGTGACTTAATCGTTACTTGCACATCAACACATTCTCGGAATTTCACATTA
    GGATATAAACTTGGACAAGGTGAATCAATGGATCAAGCATTATCTGAAATGAATATGGTT
    GTTGAAGGTATTTATACAACTAAATCAGTTTATCATTTAGCTAAAGAAAAAAATGTGGAT
    ATGCCAATTACAAATGCATTATATAGAGTATTATTTGAAAATATCTCAGTAAAAGAATGC
    GTAAAAGATTTAATGGAGCGCGATAAAAAATCTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    999

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS07710 [old locus tag: SACOL1514 ]
  • symbol: SACOL_RS07710
  • description: glycerol-3-phosphate dehydrogenase (NAD(P)(+))
  • length: 332
  • theoretical pI: 6.26285
  • theoretical MW: 36071.3
  • GRAVY: -0.0858434

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.8?  ExPASy
    Glycerol-3-phosphate dehydrogenase (NAD+) sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH
    EC 1.1.1.94?  ExPASy
    Glycerol-3-phosphate dehydrogenase (NAD(P)+) sn-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H
  • TIGRFAM:
    glycerol-3-phosphate dehydrogenase (NAD(+)) (TIGR03376; EC 1.1.1.8; HMM-score: 236.2)
    and 8 more
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 40)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 31.2)
    Metabolism Energy metabolism Electron transport NADPH-dependent F420 reductase (TIGR01915; HMM-score: 24.5)
    Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 22.4)
    Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 22)
    3-hydroxyacyl-CoA dehydrogenase PaaC (TIGR02279; EC 1.1.1.-; HMM-score: 15.5)
    Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00873; EC 1.1.1.44; HMM-score: 13.6)
    Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 13.3)
  • TheSEED: see SACOL1514
  • PFAM:
    6PGD_C (CL0106) NAD_Gly3P_dh_C; NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus (PF07479; HMM-score: 207.2)
    NADP_Rossmann (CL0063) NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 181.1)
    and 14 more
    6PGD_C (CL0106) GPD_NAD_C_bact; Bacterial GPD, NAD-dependent C-terminal (PF20618; HMM-score: 82.7)
    NADP_Rossmann (CL0063) F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 40.2)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 35)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 29.2)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 27.9)
    KARI_N; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 22.4)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 22.3)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 19.1)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 16.2)
    Nucleot_cyclase (CL0276) GCH_III; GTP cyclohydrolase III (PF05165; HMM-score: 14.6)
    NADP_Rossmann (CL0063) Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 13.9)
    PDH_N; Prephenate dehydrogenase, nucleotide-binding domain (PF02153; HMM-score: 13.9)
    6PGD_C (CL0106) ApbA_C; Ketopantoate reductase PanE/ApbA C terminal (PF08546; HMM-score: 13.4)
    NADP_Rossmann (CL0063) adh_short; short chain dehydrogenase (PF00106; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9525
    • Cytoplasmic Membrane Score: 0.0035
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0439
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 21 aa
    • SP(Sec/SPI): 0.614414
    • TAT(Tat/SPI): 0.005277
    • LIPO(Sec/SPII): 0.014003
    • Cleavage Site: CS pos: 21-22. VLA-EN. Pr: 0.5386
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCHTNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFIHVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKSVSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTRGLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSEMNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1514
  • protein localization: see SACOL1514
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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