PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS03890 [old locus tag: SACOL0757 ]
• pan locus tag^?: SAUPAN002582000
• symbol: SACOL_RS03890
• pan gene symbol^?: fruR
• synonym:
• product: DeoR/GlpR transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS03890 [old locus tag: SACOL0757 ]
• symbol: SACOL_RS03890
• product: DeoR/GlpR transcriptional regulator
• replicon: chromosome
• strand: +
• coordinates: 778780..779544
• length: 765
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (778780..779544) NCBI
• BioCyc: SACOL_RS03890 BioCyc
• MicrobesOnline: see SACOL0757

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATGATAATTACTGAAAAAAGACACGAGTTAATATTAGAAGAACTTTTGCACAAAGAT
  TTTTTGACTTTACAAGAATTAATAGATCGAACTGGTTGCAGTGCTTCAACAATACGAAGA
  GATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTA
  AAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGAATCTTGATGAA
  AAGAAAATGATTGCTAAAATAGCAGCTAATCAAATCAACGATAATGAATGCTTATTTATC
  GATGCTGGTTCATCTACATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTG
  GTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACAATTATG
  CTAGGTGGTCAAGTTAAAGAAAATACACTTGCTACGATTGGTTCTAGTGCTATGGAGATA
  TTAAGACGATATTGTTTCGATAAAGCTTTTATCGGGATGAATGGATTAGATATTGAACTT
  GGATTAACTACTCCCGATGAGCAAGAGGCATTAGTTAAACAAACAGCAATGTCATTAGCC
  AATCAATCATTTGTACTTATAGATCATTCTAAGTTTAATAAAGTATATTTTGCTCGTGTA
  CCTTTGCTAGAAAGTACGACAATCATCACATCTGAAAAAGCATTAAATCAAGAATCGTTA
  AAAGAATACCAACAAAAGTATCACTTTATAGGAGGGACTTTATGA
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⊟Protein

⊟General

• locus tag: SACOL_RS03890 [old locus tag: SACOL0757 ]
• symbol: SACOL_RS03890
• description: DeoR/GlpR transcriptional regulator
• length: 254
• theoretical pI: 7.13005
• theoretical MW: 28499
• GRAVY: -0.104331

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 16.3)
  noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 15.9)
  and 2 more
  Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 12.6)
  Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 12.6)
• TheSEED: see SACOL0757
• PFAM:
  ISOCOT_Fold (CL0246) DeoRC; DeoR C terminal sensor domain (PF00455; HMM-score: 164.4)
  and 15 more
  HTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 58.9)
  HTH_11; HTH domain (PF08279; HMM-score: 34.3)
  GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 23.7)
  HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 20.8)
  HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 19.1)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 18.2)
  Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 16.1)
  Linker_histone; linker histone H1 and H5 family (PF00538; HMM-score: 15)
  MarR_2; MarR family (PF12802; HMM-score: 14.9)
  HTH_9; RNA polymerase III subunit RPC82 helix-turn-helix domain (PF08221; HMM-score: 14.6)
  AbiEi_4; Transcriptional regulator, AbiEi antitoxin (PF13338; HMM-score: 13.6)
  HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 13.2)
  HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 13.1)
  Put_DNA-bind_N; Putative DNA-binding protein N-terminus (PF06971; HMM-score: 12.8)
  Met_repress (CL0057) SeqA_N; SeqA protein N-terminal domain (PF17206; HMM-score: 11.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by FruR*, TF important in Fructose utilization: see SACOL0757

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00363
  • TAT(Tat/SPI): 0.003088
  • LIPO(Sec/SPII): 0.005666
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: see SACOL0757
• RefSeq: WP_010956548 NCBI
• UniProt: see SACOL0757

⊟Protein sequence

• MMIITEKRHELILEELLHKDFLTLQELIDRTGCSASTIRRDLSKLQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDAGSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEILRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFARVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: see SACOL0757
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA, FruR* see SACOL0757

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications