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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000666
  • pan locus tag?: SAUPAN002582000
  • symbol: JSNZ_000666
  • pan gene symbol?: fruR
  • synonym:
  • product: DeoR/GlpR family DNA-binding transcription regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000666
  • symbol: JSNZ_000666
  • product: DeoR/GlpR family DNA-binding transcription regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 698862..699626
  • length: 765
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGATGATAATTACAGAAAAAAGACACGAGTTAATATTAGAAGAACTTTCGCACAAAGAT
    TTTTTGACTTTACAAGAATTAATAGATCGAACTGGTTGCAGTGCTTCAACAATACGAAGA
    GATTTATCTAAACTACAACAATTAGGGAAATTGCAACGTGTGCATGGTGGTGCAATGTTA
    AAAGAAAATCGTATGGTTGAGGCGAATTTAACTGAAAAATTAGCAACGAATCTTGATGAA
    AAGAAAATGATTGCTAAAATAGCAGCTAATCAAATCAACGATAATGAATGCTTATTTATC
    GATGCTGGTTCATCTACATTGGAGCTAATTAAATATATTCAAGCGAAAGATATCATTGTG
    GTAACCAATGGTTTAACACATGTAGAAGCTTTACTTAAAAAAGGTATTAAAACAATTATG
    CTAGGTGGTCAAGTTAAAGAAAATACACTTGCTACGATTGGTTCTAGTGCCATGGAGATA
    TTAAGACGATATTGTTTCGATAAAGCTTTTATCGGGATGAATGGATTAGATATTGAACTT
    GGATTAACTACTCCCGATGAGCAAGAGGCATTAGTTAAACAAACAGCAATGTCATTAGCC
    AATCAATCATTTGTACTTATAGATCATTCTAAGTTTAATAAAGTATATTTTGCTCGTGTA
    CCTTTGCTAGAAAGCACGACAATCATCACATCTGAAAAAGTATTAAATCAAGAATCGTTA
    AAAGAATACCAACAAAAGTATCACTTTATAGGAGGGACTTTATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    765

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000666
  • symbol: JSNZ_000666
  • description: DeoR/GlpR family DNA-binding transcription regulator
  • length: 254
  • theoretical pI: 7.13005
  • theoretical MW: 28501
  • GRAVY: -0.112992

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 16.5)
    noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 15.2)
    and 3 more
    Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 12.7)
    Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 12.7)
    RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 12.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) DeoRC; DeoR C terminal sensor domain (PF00455; HMM-score: 168.2)
    and 18 more
    HTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 58.2)
    HTH_11; HTH domain (PF08279; HMM-score: 35.6)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 24.9)
    HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 21.3)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 19.6)
    HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 18.5)
    HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 18.3)
    Linker_histone; linker histone H1 and H5 family (PF00538; HMM-score: 18.2)
    AbiEi_4; Transcriptional regulator, AbiEi antitoxin (PF13338; HMM-score: 16.8)
    DprA_WH; DprA winged helix domain (PF17782; HMM-score: 15.1)
    HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 15)
    Put_DNA-bind_N; Putative DNA-binding protein N-terminus (PF06971; HMM-score: 14.4)
    Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 14.2)
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 13.1)
    DnaD_N; DnaD N-terminal domain (PF21984; HMM-score: 12.7)
    MarR_2; MarR family (PF12802; HMM-score: 12.5)
    Met_repress (CL0057) SeqA_N; SeqA protein N-terminal domain (PF17206; HMM-score: 12.2)
    HTH (CL0123) HTH_9; RNA polymerase III subunit RPC82 helix-turn-helix domain (PF08221; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: Fructose-6-phosphate
  • genes regulated by FruR*, TF important in Fructose utilizationsee RegPrecise for N315
    repression
    fruR* > pfkB > fruA*

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9956
    • Cytoplasmic Membrane Score: 0.0031
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0011
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003593
    • TAT(Tat/SPI): 0.002721
    • LIPO(Sec/SPII): 0.005079
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSKLQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDAGSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEILRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFARVPLLESTTIITSEKVLNQESLKEYQQKYHFIGGTL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: FruR* (repression) regulon, CcpA regulon
    FruR*(TF)important in Fructose utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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