PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000667
• pan locus tag^?: SAUPAN002583000
• symbol: pfkB
• pan gene symbol^?: fruB
• synonym:
• product: 1-phosphofructokinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000667
• symbol: pfkB
• product: 1-phosphofructokinase
• replicon: chromosome
• strand: +
• coordinates: 699623..700543
• length: 921
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGATTTATACAGTGACTTTCAATCCTTCAATTGACTATGTCATTTTTACTAATGATTTT
  AAAATTGATGGTTTGAACAGAGCAACAGCAACATATAAATTCGCTGGGGGAAAAGGTATT
  AATGTCTCACGTGTTTTAAAGACATTGGATGTTGAATCAACTGCTTTGGGATTTGCAGGT
  GGATTTCCTGGGAAATTCATTATAGATACATTAAATAACAGTGCAATTCAATCGAATTTT
  ATTGAAGTTGATGAAGATACACGTATTAATGTGAAATTAAAAACAGGACAAGAAACAGAA
  ATCAATGCACCGGGTCCTCATATAACGTCAACACAATTTGAACAACTGTTACAACAAATT
  AAAAATACAACAAGCGAAGATATAGTTATTGTTGCTGGAAGTGTACCAAGTAGTATTCCA
  AGCGATGCGTATGCGCAAATTGCACAAATTACAGCACAGACAGGTGCTAAATTAGTAGTC
  GACGCTGAAAAAGAATTGGCTGAAAGCGTTTTACCATATCATCCACTATTTATTAAACCT
  AATAAAGATGAATTAGAAGTGATGTTTAATACAACAGTGAACTCAGACGAAGATGTTATT
  AAATATGGTCGTTTATTAGTTGATAAAGGTGCGCAATCTGTTATTGTCTCACTTGGCGGT
  GATGGTGCTATTTATATTGATAAAGAAATCAGTATTAAAGCAGTTAATCCACAAGGGAAA
  GTGGTTAATACAGTTGGCTCTGGTGATAGTACAGTTGCAGGCATGGTGGCTGGAATTGCT
  TCAGGTTTAACGATTGAAAAAGCATTCCAACAAGCAGTTGCATGCGGTACTGCCACGGCA
  TTTGATGAGGACTTAGCAACACGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATT
  AGCGTACTTGATGGGGAGTGA
  60
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  921
  

⊟Protein

⊟General

• locus tag: JSNZ_000667
• symbol: PfkB
• description: 1-phosphofructokinase
• length: 306
• theoretical pI: 4.35598
• theoretical MW: 32600.6
• GRAVY: 0.0156863

⊟Function

• reaction:
  EC 2.7.1.56?  ExPASy
  1-phosphofructokinase ATP + D-fructose 1-phosphate = ADP + D-fructose 1,6-bisphosphate
• TIGRFAM:
  1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 356.7)
  hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 355.8)
  and 6 more
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides tagatose-6-phosphate kinase (TIGR01231; EC 2.7.1.144; HMM-score: 177.5)
  Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 67.1)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 53.9)
  Metabolism Energy metabolism Sugars 5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 43.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 37.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 23.5)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 187.3)
  and 6 more
  Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 37.1)
  Carb_kinase; NAD(P)H-hydrate dehydratase (PF01256; HMM-score: 25.3)
  NADP_Rossmann (CL0063) GRDA; Glycine reductase complex selenoprotein A (PF04723; HMM-score: 14.3)
  Beta_propeller (CL0186) Mala_s_1-like; Mal s 1 allergenic protein-like (PF22701; HMM-score: 14.2)
  HTH (CL0123) PuR_N; Bacterial purine repressor, N-terminal (PF09182; HMM-score: 13.6)
  no clan defined Cas_Csa5; CRISPR-associated protein (Cas_Csa5) (PF09702; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9823
  • Cytoplasmic Membrane Score: 0.0119
  • Cell wall & surface Score: 0.0014
  • Extracellular Score: 0.0044
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010086
  • TAT(Tat/SPI): 0.00061
  • LIPO(Sec/SPII): 0.000526
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSRVLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEINAPGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVVDAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDEDVIKYGRLLVDKGAQSVIVSLGGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACGTATAFDEDLATRDAIEKIKSQVTISVLDGE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications