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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS03750 [old locus tag: SACOL0730 ]
- pan locus tag?: SAUPAN002553000
- symbol: SACOL_RS03750
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS03750 [old locus tag: SACOL0730 ]
- symbol: SACOL_RS03750
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 754818..755462
- length: 645
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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541
601GTGAAAGTAAAGTATATAGATAAACGTCACTGGCGTCGCCTAATTGATAGGGAATACACA
GAGGTAAAAGTTAATAATAATAGGTTTAAGGGTATTATAGGCTTAGTCACGATGAAAAAG
GTTCGTGATCCTTTAGAGGTGACGGTAGTTGGACAAAATATCATTGTCGCAGATGACAAT
TATAAATGGTTGCAAATACTACCTGAAAAGAAACGTTATAGTATAACTGTAATGTTTGAT
AATAAAGGCAATCCATTAGAATATTATTTTGATATAAATATCAAAAATATAACGCAAAAA
GGTAATGCGCGTACAGTAGATTTATGTTTAGATGTTTTAGCATTACCAAGTGGTGAATAT
GAGTTGGTAGATGAAGATGATTTAATGTTTGCATTAGAAAGTGAGCAAATTACAAAAAAG
CAATTTCATGAAGCATATATGATTGCACATCAAATTATGGCAGAGTTAGAAAATGATTTT
AAAGGATTCCAAAAGAAAATCATGTACTGCTTTAATAAAATTAATGCAAAGGCTCAAAAA
AATCATCAAAAGCCACAAAATAAAACTAATATTGAAAAAAGCAAACAAATAAAGCCTAAG
CAATATAATCAAACTAAAAATCACCAACAACAAAAGAAAAACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS03750 [old locus tag: SACOL0730 ]
- symbol: SACOL_RS03750
- description: hypothetical protein
- length: 214
- theoretical pI: 9.96721
- theoretical MW: 25416.2
- GRAVY: -0.827103
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: see SACOL0730
- PFAM: no clan defined DUF402; Protein of unknown function (DUF402) (PF04167; HMM-score: 62.4)and 2 moreSAS-6_N; Centriolar protein SAS N-terminal (PF16531; HMM-score: 14.4)ABC_transp_aux; ABC-type uncharacterized transport system (PF09822; HMM-score: 13.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003042
- TAT(Tat/SPI): 0.000171
- LIPO(Sec/SPII): 0.000447
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDPLEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGNARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDFKGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization: see SACOL0730
- quantitative data / protein copy number per cell: see SACOL0730
- interaction partners:
SACOL_RS01530 5'-nucleotidase, lipoprotein e(P4) family [1] (data from MRSA252) SACOL_RS06420 50S ribosomal protein L19 [1] (data from MRSA252) SACOL_RS07705 DNA-binding protein HU [1] (data from MRSA252) SACOL_RS08830 translation initiation factor IF-3 [1] (data from MRSA252) SACOL_RS08970 universal stress protein [1] (data from MRSA252) SACOL_RS09045 30S ribosomal protein S4 [1] (data from MRSA252) SACOL_RS11615 30S ribosomal protein S9 [1] (data from MRSA252) SACOL_RS11705 50S ribosomal protein L6 [1] (data from MRSA252) SACOL_RS11735 30S ribosomal protein S17 [1] (data from MRSA252) SACOL_RS11745 50S ribosomal protein L16 [1] (data from MRSA252) SACOL_RS11755 50S ribosomal protein L22 [1] (data from MRSA252) SACOL_RS11770 50S ribosomal protein L23 [1] (data from MRSA252) SACOL_RS11775 50S ribosomal protein L4 [1] (data from MRSA252) SACOL_RS11780 50S ribosomal protein L3 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CodY see SACOL0730
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)