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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

organism: Staphylococcus aureus COL
locus tag: SACOL2712 [new locus tag: SACOL_RS14185 ]
pan locus tag^?: SAUPAN006445000
symbol: drp35
pan gene symbol^?: drp35
synonym:
product: drP35 protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SACOL2712 [new locus tag: SACOL_RS14185 ]
symbol: drp35
product: drP35 protein
replicon: chromosome
strand: -
coordinates: 2784346..2785317
length: 972
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID: 3238077 NCBI
RefSeq: YP_187498 NCBI
BioCyc: see SACOL_RS14185
MicrobesOnline: 914178 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
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481
541
601
661
721
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841
901
961
ATGTCACAACAAGATTTACCTACATTATTTTATAGCGGGAAGTCCAATAGTGCTGTTCCA
ATTATATCTGAAAGTGAATTACAAACAATTACAGCTGAACCATGGCTTGAAATTTCCAAA
AAAGGATTGCAACTAGAAGGATTGAACTTTGATCGGCAGGGACAACTCTTTTTATTGGAT
GTATTCGAAGGCAATATTTTCAAAATCAATCCTGAAACGAAGGAAATCAAACGACCTTTT
GTAAGTCACAAAGCGAATCCTGCAGCAATCAAAATACATAAAGATGGCCGATTATTCGTT
TGTTATTTAGGAGATTTTAAATCTACAGGAGGCATTTTTGCAGCTACAGAAAATGGTGAC
AACTTACAAGATATTATTGAAGATCTTTCAACAGCATATTGTATTGATGACATGGTATTT
GATTCTAAAGGTGGATTTTATTTTACAGATTTTAGAGGATACTCTACCAATCCACTAGGA
GGCGTTTATTATGTTTCGCCGGACTTTAGAACAGTGACGCCTATCATTCAAAATATTAGC
GTAGCAAATGGTATTGCTTTAAGTACAGATGAAAAAGTACTATGGGTAACAGAAACAACA
GCCAATCGATTACATCGCATTGCACTTGAAGATGATGGTGTGACGATACAACCATTTGGA
GCTACCATACCGTACTATTTTACAGGTCATGAAGGACCAGACTCATGTTGTATTGATAGT
GACGATAATTTATACGTAGCAATGTATGGTCAAGGTCGAGTGTTAGTTTTTAATAAAAGG
GGTTATCCAATAGGACAAATATTGATACCAGGCCGAGATGAAGGGCATATGTTACGTTCT
ACTCATCCGCAATTTATACCTGGAACAAATCAACTCATCATTTGTTCCAATGATATAGAA
ATGGGCGGAGGGTCTATGCTTTATACAGTTAATGGCTTTGCAAAAGGTCATCAAAGTTTT
CAGTTTCAATAA
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972

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SACOL2712 [new locus tag: SACOL_RS14185 ]
symbol: Drp35
description: drP35 protein
length: 323
theoretical pI: 4.71521
theoretical MW: 35832.2
GRAVY: -0.19257

⊟Function[edit | edit source]

reaction:
EC 3.1.1.-? ExPASy
TIGRFAM:
TheSEED :
- Lactonase Drp35
PFAM:
Beta_propeller (CL0186) SGL; SMP-30/Gluconolaconase/LRE-like region (PF08450; HMM-score: 102.2)
and 3 more
Arylesterase; Arylesterase (PF01731; HMM-score: 28.9)
Str_synth; Strictosidine synthase (PF03088; HMM-score: 27.7)
NHL; NHL repeat (PF01436; HMM-score: 23)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors: Ca²⁺
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 10
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 0
LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003495
- TAT(Tat/SPI): 0.000154
- LIPO(Sec/SPII): 0.000415
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 57651080 NCBI
RefSeq: YP_187498 NCBI
UniProt: Q5HCK9 UniProt

⊟Protein sequence[edit | edit source]

MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQLEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCYLGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLGGVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQPFGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGHMLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ

⊟Experimental data[edit | edit source]

experimentally validated: PeptideAtlas
protein localization: Cytoplasmic ^[1] ^[2] ^[3]
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

MicrobesOnline: no polycistronic organisation predicted

⊟Regulation[edit | edit source]

regulator: CcpA regulon
CcpA (TF) important in Carbon catabolism; RegPrecise

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e)
↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p)
↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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