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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1897 [new locus tag: SACOL_RS09770 ]
  • pan locus tag?: SAUPAN004771000
  • symbol: SACOL1897
  • pan gene symbol?: prsA
  • synonym:
  • product: protein export protein PrsA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1897 [new locus tag: SACOL_RS09770 ]
  • symbol: SACOL1897
  • product: protein export protein PrsA
  • replicon: chromosome
  • strand: +
  • coordinates: 1950911..1951873
  • length: 963
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGATGATAAACAAATTAATCGTTCCGGTAACAGCTAGTGCTTTATTATTAGGCGCT
    TGTGGCGCTAGTGCCACAGACTCTAAAGAAAATACATTAATTTCTTCTAAAGCTGGAGAC
    GTAACAGTTGCAGATACAATGAAAAAAATCGGTAAAGATCAAATTGCAAATGCATCATTT
    ACTGAAATGTTAAATAAAATTTTAGCTGATAAATATAAAAATAAAGTTAATGATAAGAAG
    ATTGACGAACAAATTGAAAAAATGCAAAAGCAATACGGCGGTAAAGATAAATTTGAAAAG
    GCCCTTCAACAGCAAGGTTTAACAGCCGATAAATATAAAGAAAATTTACGTACTGCTGCT
    TATCATAAAGAATTACTATCAGATAAAATTAAAATCTCTGATTCTGAAATTAAAGAAGAC
    AGCAAGAAAGCTTCACACATTTTAATTAAAGTTAAATCTAAGAAAAGCGACAAAGAAGGC
    TTAGATGATAAAGAAGCGAAACAAAAAGCTGAAGAAATTCAAAAAGAAGTTTCAAAAGAT
    CCAAGTAAATTTGGTGAAATCGCTAAAAAAGAATCAATGGATACTGGTTCAGCTAAAAAA
    GATGGCGAATTAGGTTATGTTCTTAAAGGACAAACTGATAAAGATTTTGAAAAAGCACTA
    TTTAAGCTTAAAGATGGTGAAGTATCAGAGGTTGTTAAATCAAGCTTTGGATATCATATT
    ATTAAAGCTGATAAACCAACAGACTTTAACAGTGAAAAACAAAGCCTGAAAGAAAAATTA
    GTCGATCAGAAAGTACAAAAAAATCCAAAATTATTGACTGATGCATACAAAGATCTATTA
    AAAGAATACGATGTTGACTTTAAAGATCGTGATATTAAATCAGTTGTCGAAGATAAAATC
    TTAAACCCTGAAAAACTTAAACAAGGTGGCGCACAAGGCGGACAATCCGGCATGAGCCAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    963

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1897 [new locus tag: SACOL_RS09770 ]
  • symbol: SACOL1897
  • description: protein export protein PrsA
  • length: 320
  • theoretical pI: 9.6463
  • theoretical MW: 35637.5
  • GRAVY: -0.858125

⊟Function[edit | edit source]

  • reaction:
    EC 5.2.1.8?  ExPASy
    Peptidylprolyl isomerase Peptidylproline (omega=180) = peptidylproline (omega=0)
  • TIGRFAM:
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation protein NifM (TIGR02933; HMM-score: 49.5)
  • TheSEED  :
    • Foldase clustered with pyrimidine conversion
    • Foldase protein PrsA (EC 5.2.1.8)
    Membrane Transport Protein translocation across cytoplasmic membrane EcsAB transporter affecting expression and secretion of secretory preproteins  Foldase protein PrsA precursor (EC 5.2.1.8)
    and 1 more
    Protein Metabolism Protein folding Peptidyl-prolyl cis-trans isomerase  Foldase protein PrsA precursor (EC 5.2.1.8)
  • PFAM:
    FKBP (CL0487) Rotamase; PPIC-type PPIASE domain (PF00639; HMM-score: 99.2)
    Rotamase_3; PPIC-type PPIASE domain (PF13616; HMM-score: 94.2)
    and 4 more
    Rotamase_2; PPIC-type PPIASE domain (PF13145; HMM-score: 32.9)
    Trigger_C (CL0262) SurA_N_3; SurA N-terminal domain (PF13624; HMM-score: 24.5)
    SurA_N; SurA N-terminal domain (PF09312; HMM-score: 16.2)
    HTH (CL0123) Fe_dep_repr_C; Iron dependent repressor, metal binding and dimerisation domain (PF02742; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LLLGACG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.01518
    • TAT(Tat/SPI): 0.000182
    • LIPO(Sec/SPII): 0.984206
    • Cleavage Site: CS pos: 20-21. LGA-CG. Pr: 0.9847
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVADTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKALQQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEGLDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEKALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAYKDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Lipoprotein [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 14.42 h [5]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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