PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1790 [new locus tag: SAUSA300_RS09800 ]
• pan locus tag^?: SAUPAN004771000
• symbol: prsA
• pan gene symbol^?: prsA
• synonym:
• product: foldase protein PrsA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1790 [new locus tag: SAUSA300_RS09800 ]
• symbol: prsA
• product: foldase protein PrsA
• replicon: chromosome
• strand: +
• coordinates: 1971557..1972519
• length: 963
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914205 NCBI
• RefSeq: YP_494473 NCBI
• BioCyc: see SAUSA300_RS09800
• MicrobesOnline: 1293305 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
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  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  ATGAAGATGATAAACAAATTAATCGTTCCGGTAACAGCTAGTGCTTTATTATTAGGCGCT
  TGTGGCGCTAGTGCCACAGACTCTAAAGAAAATACATTAATTTCTTCTAAAGCTGGAGAC
  GTAACAGTTGCAGATACAATGAAAAAAATCGGTAAAGATCAAATTGCAAATGCATCATTT
  ACTGAAATGTTAAATAAAATTTTAGCTGATAAATATAAAAATAAAGTTAATGATAAGAAG
  ATTGACGAACAAATTGAAAAAATGCAAAAGCAATACGGCGGTAAAGATAAATTTGAAAAG
  GCCCTTCAACAGCAAGGTTTAACAGCCGATAAATATAAAGAAAATTTACGTACTGCTGCT
  TATCATAAAGAATTACTATCAGATAAAATTAAAATCTCTGATTCTGAAATTAAAGAAGAC
  AGCAAGAAAGCTTCACACATTTTAATTAAAGTTAAATCTAAGAAAAGCGACAAAGAAGGC
  TTAGATGATAAAGAAGCGAAACAAAAAGCTGAAGAAATTCAAAAAGAAGTTTCAAAAGAT
  CCAAGTAAATTTGGTGAAATCGCTAAAAAAGAATCAATGGATACTGGTTCAGCTAAAAAA
  GATGGCGAATTAGGTTATGTTCTTAAAGGACAAACTGATAAAGATTTTGAAAAAGCACTA
  TTTAAGCTTAAAGATGGTGAAGTATCAGAGGTTGTTAAATCAAGCTTTGGATATCATATT
  ATTAAAGCTGATAAACCAACAGACTTTAACAGTGAAAAACAAAGCCTGAAAGAAAAATTA
  GTCGATCAGAAAGTACAAAAAAATCCAAAATTATTGACTGATGCATACAAAGATCTATTA
  AAAGAATACGATGTTGACTTTAAAGATCGTGATATTAAATCAGTTGTCGAAGATAAAATC
  TTAAACCCTGAAAAACTTAAACAAGGTGGCGCACAAGGCGGACAATCCGGCATGAGCCAA
  TAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1790 [new locus tag: SAUSA300_RS09800 ]
• symbol: PrsA
• description: foldase protein PrsA
• length: 320
• theoretical pI: 9.6463
• theoretical MW: 35637.5
• GRAVY: -0.858125

⊟Function

• reaction:
  EC 5.2.1.8?  ExPASy
  Peptidylprolyl isomerase Peptidylproline (omega=180) = peptidylproline (omega=0)
• TIGRFAM:
  Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation protein NifM (TIGR02933; HMM-score: 49.5)
• TheSEED  :
  • Foldase clustered with pyrimidine conversion
  • Foldase protein PrsA (EC 5.2.1.8)
  Membrane Transport Protein translocation across cytoplasmic membrane EcsAB transporter affecting expression and secretion of secretory preproteins  Foldase protein PrsA precursor (EC 5.2.1.8)
  and 1 more
  Protein Metabolism Protein folding Peptidyl-prolyl cis-trans isomerase  Foldase protein PrsA precursor (EC 5.2.1.8)
• PFAM:
  FKBP (CL0487) Rotamase; PPIC-type PPIASE domain (PF00639; HMM-score: 99.2)
  Rotamase_3; PPIC-type PPIASE domain (PF13616; HMM-score: 94.2)
  and 4 more
  Rotamase_2; PPIC-type PPIASE domain (PF13145; HMM-score: 32.9)
  Trigger_C (CL0262) SurA_N_3; SurA N-terminal domain (PF13624; HMM-score: 24.5)
  SurA_N; SurA N-terminal domain (PF09312; HMM-score: 16.2)
  HTH (CL0123) Fe_dep_repr_C; Iron dependent repressor, metal binding and dimerisation domain (PF02742; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.68
  • Cellwall Score: 0.17
  • Extracellular Score: 0.16
  • Internal Helices: 0
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: -0.33
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: LLLGACG
• SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
  • SP(Sec/SPI): 0.01518
  • TAT(Tat/SPI): 0.000182
  • LIPO(Sec/SPII): 0.984206
  • Cleavage Site: CS pos: 20-21. LGA-CG. Pr: 0.9847
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162356 NCBI
• RefSeq: YP_494473 NCBI
• UniProt: Q2FFQ5 UniProt

⊟Protein sequence

• MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVADTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKALQQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEGLDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEKALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAYKDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications