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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01972
  • pan locus tag?: SAUPAN004771000
  • symbol: SAOUHSC_01972
  • pan gene symbol?: prsA
  • synonym:
  • product: protein export protein PrsA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01972
  • symbol: SAOUHSC_01972
  • product: protein export protein PrsA
  • replicon: chromosome
  • strand: +
  • coordinates: 1875249..1876211
  • length: 963
  • essential: no [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGATGATAAACAAATTAATCGTTCCGGTAACAGCTAGTGCTTTATTATTAGGCGCT
    TGTGGCGCTAGTGCCACAGACTCTAAAGAAAATACATTAATTTCTTCTAAAGCTGGAGAC
    GTAACAGTTGCAGATACAATGAAAAAAATCGGTAAAGATCAAATTGCAAATGCATCATTT
    ACTGAAATGTTAAATAAAATTTTAGCTGATAAATATAAAAATAAAGTTAATGATAAGAAG
    ATTGACGAACAAATTGAAAAAATGCAAAAGCAATACGGCGGTAAAGATAAATTTGAAAAG
    GCCCTTCAACAGCAAGGTTTAACAGCCGATAAATATAAAGAAAATTTACGTACTGCTGCT
    TATCATAAAGAATTACTATCAGATAAAATTAAAATCTCTGATTCTGAAATTAAAGAAGAC
    AGCAAGAAAGCTTCACACATTTTAATTAAAGTTAAATCTAAGAAAAGCGACAAAGAAGGC
    TTAGATGATAAAGAAGCGAAACAAAAAGCTGAAGAAATTCAAAAAGAAGTTTCAAAAGAT
    CCAAGTAAATTTGGTGAAATCGCTAAAAAAGAATCAATGGATACTGGTTCAGCTAAAAAA
    GATGGCGAATTAGGTTATGTTCTTAAAGGACAAACTGATAAAGATTTTGAAAAAGCACTA
    TTTAAGCTTAAAGATGGTGAAGTATCAGAGGTTGTTAAATCAAGCTTTGGATATCATATT
    ATTAAAGCTGATAAACCAACAGACTTTAACAGTGAAAAACAAAGCCTGAAAGAAAAATTA
    GTCGATCAGAAAGTACAAAAAAATCCAAAATTATTGACTGATGCATACAAAGATCTATTA
    AAAGAATACGATGTTGACTTTAAAGATCGTGATATTAAATCAGTTGTCGAAGATAAAATC
    TTAAACCCTGAAAAACTTAAACAAGGTGGCGCACAAGGCGGACAATCCGGCATGAGCCAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    963

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01972
  • symbol: SAOUHSC_01972
  • description: protein export protein PrsA
  • length: 320
  • theoretical pI: 9.6463
  • theoretical MW: 35637.5
  • GRAVY: -0.858125

⊟Function[edit | edit source]

  • reaction:
    EC 5.2.1.8?  ExPASy
    Peptidylprolyl isomerase Peptidylproline (omega=180) = peptidylproline (omega=0)
  • TIGRFAM:
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation protein NifM (TIGR02933; HMM-score: 49.5)
  • TheSEED  :
    • Foldase clustered with pyrimidine conversion
    • Foldase protein PrsA (EC 5.2.1.8)
    Membrane Transport Protein translocation across cytoplasmic membrane EcsAB transporter affecting expression and secretion of secretory preproteins  Foldase protein PrsA precursor (EC 5.2.1.8)
    and 1 more
    Protein Metabolism Protein folding Peptidyl-prolyl cis-trans isomerase  Foldase protein PrsA precursor (EC 5.2.1.8)
  • PFAM:
    FKBP (CL0487) Rotamase; PPIC-type PPIASE domain (PF00639; HMM-score: 99.2)
    Rotamase_3; PPIC-type PPIASE domain (PF13616; HMM-score: 94.2)
    and 4 more
    Rotamase_2; PPIC-type PPIASE domain (PF13145; HMM-score: 32.9)
    Trigger_C (CL0262) SurA_N_3; SurA N-terminal domain (PF13624; HMM-score: 24.5)
    SurA_N; SurA N-terminal domain (PF09312; HMM-score: 16.2)
    HTH (CL0123) Fe_dep_repr_C; Iron dependent repressor, metal binding and dimerisation domain (PF02742; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LLLGACG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.01518
    • TAT(Tat/SPI): 0.000182
    • LIPO(Sec/SPII): 0.984206
    • Cleavage Site: CS pos: 20-21. LGA-CG. Pr: 0.9847
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVADTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKALQQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEGLDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEKALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAYKDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Ambre Jousselin, Adriana Renzoni, Diego O Andrey, Antoinette Monod, Daniel P Lew, William L Kelley
The posttranslocational chaperone lipoprotein PrsA is involved in both glycopeptide and oxacillin resistance in Staphylococcus aureus.
Antimicrob Agents Chemother: 2012, 56(7);3629-40
[PubMed:22526301] [WorldCat.org] [DOI] (I p)

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