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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1694 [new locus tag: SACOL_RS08640 ]
  • pan locus tag?: SAUPAN004245000
  • symbol: tgt
  • pan gene symbol?: tgt
  • synonym:
  • product: queuine tRNA-ribosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1694 [new locus tag: SACOL_RS08640 ]
  • symbol: tgt
  • product: queuine tRNA-ribosyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1726575..1727714
  • length: 1140
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCCTGCAGTAACATACGAACACATTAAAACTTGTAAACAATCAGGTGCGCGTTTAGGT
    ATCGTGCACACACCACACGGTTCATTTGAAACACCTATGTTTATGCCAGTTGGTACTAAA
    GCAACCGTTAAAACAATGAGTCCAGAAGAGTTAAGACAAATTGAAGCAAAAATCATTTTG
    GGCAACACATATCATTTGTGGTTACAACCCGGAAATGATATTATCAAACACGCTGGGGGA
    TTACATAAATTCATGAATTGGGATGGTCCGATTCTTACAGATTCAGGCGGTTTCCAAGTG
    TTTAGTTTAAGTAATTTACGTAAAATTACAGAAGAAGGCGTGGAATTTAGACATCATACT
    AATGGGTCTAAATTATTTTTGAGTCCTGAGAAATCAATGCAAATTCAAAATGATTTAGGA
    TCTGATATTATGATGGCATTTGATGAATGTCCACCGATGCCTGCTGAATATGATTATGTA
    AAAAAATCTATTGAACGTACAACACGTTGGGCGAAAAGATGTCTAGATGCACACCAAAGA
    CCTGAAGATCAAGCATTGTTCGGCATTATACAAGGTGGCGAATATGAAGATTTAAGAGAA
    CAAAGTGCAAAGGATTTAGTAGAATTAGATTTTCCTGGTTATGCAATCGGCGGTTTGTCA
    GTTGGTGAACCTAAACCGGTTATGTATAAAATGGTTGAACATACAGAGCAGTTTATGCCT
    AAAGATAAACCAAGATATTTAATGGGTGTAGGATCTCCAGATGCGTTAATCGAATGTAGT
    ATTCGCGGCATGGATATGTTTGATTGTGTCTTACCGACACGTATTGCCAGAAATGGTACT
    TGTATGACATCGCAAGGTCGTTTAGTTATTAAAAATGCAAAATTTGCAGATGATTTAAGA
    CCGTTAGATGAGAATTGTGACTGTTATACATGTCAAAACTATTCAAGAGCGTATATACGT
    CATTTAATCAAGGCAGAGGAAACTTTTGGTATTCGTCTTACTACTATTCATAATTTACAT
    TTTCTGCTAAAATTAATGGAAGATATAAGACAAGCCATTCGAGAAGATCGTCTTTTAGAT
    TTCAAAGAAGAATTCTTCGAGCAATATGGATTAAATGTTGAGAACCCAAAAAACTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1694 [new locus tag: SACOL_RS08640 ]
  • symbol: Tgt
  • description: queuine tRNA-ribosyltransferase
  • length: 379
  • theoretical pI: 6.3237
  • theoretical MW: 43309.5
  • GRAVY: -0.435356

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.29?  ExPASy
    tRNA-guanine34 transglycosylase Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA-guanine transglycosylase (TIGR00430; EC 2.4.2.29; HMM-score: 607.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA-guanine family transglycosylase (TIGR00449; EC 2.4.2.-; HMM-score: 575.3)
    and 1 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA-guanine(15) transglycosylase (TIGR00432; EC 2.4.2.48; HMM-score: 121.7)
  • TheSEED  :
    • tRNA-guanosine(34) preQ1 transglycosylase (EC 2.4.2.29)
    RNA Metabolism RNA processing and modification Queuosine-Archaeosine Biosynthesis  tRNA guanosine(34) transglycosylase (EC 2.4.2.29)
    and 1 more
    RNA Metabolism RNA processing and modification Queuosine-Archaeosine Biosynthesis  tRNA-guanine transglycosylase (EC 2.4.2.29)
  • PFAM:
    no clan defined TGT; Queuine tRNA-ribosyltransferase (PF01702; HMM-score: 319.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008848
    • TAT(Tat/SPI): 0.001214
    • LIPO(Sec/SPII): 0.001649
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYVKKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 551 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 16.85 h [5]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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