PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1541 [new locus tag: NWMN_RS08645 ]
• pan locus tag^?: SAUPAN004245000
• symbol: tgt
• pan gene symbol^?: tgt
• synonym:
• product: queuine tRNA-ribosyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1541 [new locus tag: NWMN_RS08645 ]
• symbol: tgt
• product: queuine tRNA-ribosyltransferase
• replicon: chromosome
• strand: -
• coordinates: 1709414..1710553
• length: 1140
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330921 NCBI
• RefSeq: YP_001332575 NCBI
• BioCyc:
• MicrobesOnline: 3707093 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGCCTGCAGTAACATACGAACACATTAAAACTTGTAAACAATCAGGTGCGCGTTTAGGT
  ATCGTGCACACACCACACGGTTCATTTGAAACACCTATGTTTATGCCAGTTGGTACTAAA
  GCAACCGTTAAAACAATGAGTCCAGAAGAGTTAAGACAAATTGAAGCAAAAATCATTTTG
  GGCAACACATATCATTTGTGGTTACAACCCGGAAATGATATTATCAAACACGCTGGGGGA
  TTACATAAATTCATGAATTGGGATGGTCCGATTCTTACAGATTCAGGCGGTTTCCAAGTG
  TTTAGTTTAAGTAATTTACGTAAAATTACAGAAGAAGGCGTGGAATTTAGACATCATACT
  AATGGGTCTAAATTATTTTTGAGTCCTGAGAAATCAATGCAAATTCAAAATGATTTAGGA
  TCTGATATTATGATGGCATTTGATGAATGTCCACCGATGCCTGCTGAATATGATTATGTA
  AAAAAATCTATTGAACGTACAACACGTTGGGCGAAAAGATGTCTAGATGCACACCAAAGA
  CCTGAAGATCAAGCATTGTTCGGCATTATACAAGGTGGCGAATATGAAGATTTAAGAGAA
  CAAAGTGCAAAGGATTTAGTAGAATTAGATTTTCCTGGTTATGCAATCGGCGGTTTGTCA
  GTTGGTGAACCTAAACCGGTTATGTATAAAATGGTTGAACATACAGAGCAGTTTATGCCT
  AAAGATAAACCAAGATATTTAATGGGTGTAGGATCTCCAGATGCGTTAATCGAATGTAGT
  ATTCGCGGCATGGATATGTTTGATTGTGTCTTACCGACACGTATTGCCAGAAATGGTACT
  TGTATGACATCGCAAGGTCGTTTAGTTATTAAAAATGCAAAATTTGCAGATGATTTAAGA
  CCGTTAGATGAGAATTGTGACTGTTATACATGTCAAAACTATTCAAGAGCGTATATACGT
  CATTTAATCAAGGCAGAGGAAACTTTTGGTATTCGTCTTACTACTATTCATAATTTACAT
  TTTCTGCTAAAATTAATGGAAGATATAAGACAAGCCATTCGAGAAGATCGTCTTTTAGAT
  TTCAAAGAAGAATTCTTCGAGCAATATGGATTAAATGTTGAGAACCCAAAAAACTTTTAA
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⊟Protein

⊟General

• locus tag: NWMN_1541 [new locus tag: NWMN_RS08645 ]
• symbol: Tgt
• description: queuine tRNA-ribosyltransferase
• length: 379
• theoretical pI: 6.3237
• theoretical MW: 43309.5
• GRAVY: -0.435356

⊟Function

• reaction:
  EC 2.4.2.29?  ExPASy
  tRNA-guanine³⁴ transglycosylase Guanine³⁴ in tRNA + queuine = queuosine³⁴ in tRNA + guanine Guanine³⁴ in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine³⁴ in tRNA + guanine
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA-guanine transglycosylase (TIGR00430; EC 2.4.2.29; HMM-score: 607.8)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA-guanine family transglycosylase (TIGR00449; EC 2.4.2.-; HMM-score: 575.3)
  and 1 more
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA-guanine(15) transglycosylase (TIGR00432; EC 2.4.2.48; HMM-score: 121.7)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined TGT; Queuine tRNA-ribosyltransferase (PF01702; HMM-score: 319.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008848
  • TAT(Tat/SPI): 0.001214
  • LIPO(Sec/SPII): 0.001649
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221753 NCBI
• RefSeq: YP_001332575 NCBI
• UniProt: A6QHI1 UniProt

⊟Protein sequence

• MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYVKKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1540 < tgt < queA < ruvB < ruvA < NWMN_1545 < obgE

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications