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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1295 [new locus tag: SACOL_RS06610 ]
  • pan locus tag?: SAUPAN003577000
  • symbol: SACOL1295
  • pan gene symbol?: spoIIIE
  • synonym:
  • product: FtsK/SpoIIIE family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1295 [new locus tag: SACOL_RS06610 ]
  • symbol: SACOL1295
  • product: FtsK/SpoIIIE family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1310117..1312495
  • length: 2379
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    GTGCGTCAATTGGCACAAGCAAAAAAGAAATCGACAGCTAAGAAAAAAACAACATCAAAA
    AAAAGAACAAATTCGAGGAAAAAGAAGAATGATAATCCGATACGTTATGTCATAGCTATT
    TTAGTAGTTGTATTAATGGTGTTGGGTGTTTTCCAATTAGGAATAATAGGTCGTCTAATT
    GACAGCTTCTTTAATTATTTATTTGGGTACAGTAGATATTTAACATATATTTTAGTACTC
    TTAGCAACTGGTTTTATTACATACTCTAAACGTATTCCTAAAACTAGACGAACGGCTGGT
    TCGATTGTATTGCAAATTGCATTGCTATTTGTATCACAGTTAGTTTTTCATTTTAATAGT
    GGTATCAAAGCTGAAAGAGAACCTGTACTTTCTTATGTGTATCAGTCATACCAACACAGT
    CATTTCCCAAATTTTGGTGGCGGTGTATTAGGCTTTTATTTATTAGAGTTAAGCGTACCT
    TTAATTTCATTATTTGGTGTATGTATTATTACTATTTTATTATTATGCTCAAGTGTTATT
    TTATTAACAAACCATCAACATCGTGAAGTTGCAAAAGTTGCACTGGAAAATATAAAAGCT
    TGGTTTGGTTCATTTAATGAAAAAATGTCGGAAAGAAACCAAGAAAAACAATTGAAGCGT
    GAAGAAAAAGCAAGACTTAAAGAAGAACAAAAGGCACGTCAAAATGAACAGCCACAAATA
    AAAGATGTGAGTGATTTTACGGAAGTGCCTCAAGAAAGAGATATTCCAATTTATGGGCAT
    ACTGAAAATGAAAGTAAAAGCCAGAGTCAACCAAGTCGAAAAAAACGAGTGTTTGATGCA
    GAGAATAGTTCGAATAACATCGTAAATCATCATCAAGCAGATCAGCAAGAACAATTAACA
    GAACAAACTCATAACAGTGTTGAAAGTGAAAACACTATTGAAGAAGCTGGTGAAGTTACG
    AATGTATCGTATGTTGTTCCACCGTTAACTTTACTTAATCAACCTGCAAAACAAAAAGCA
    ACATCTAAAGCTGAAGTGCAACGTAAAGGACAAGTACTAGAGAATACATTAAAAGATTTT
    GGGGTAAATGCAAAAGTGACACAAATTAAAATTGGTCCTGCAGTAACTCAATATGAAATT
    CAACCAGCTCAAGGGGTTAAAGTGAGTAAAATTGTAAACTTGCATAATGATATTGCATTA
    GCTTTAGCAGCAAAAGATGTTAGAATCGAAGCGCCAATACCTGGTCGTTCTGCAGTAGGT
    ATTGAAGTGCCAAATGAGAAAATTTCATTAGTTTCACTAAAAGAAGTTTTAGATGAAAAA
    TTCCCGTCTAATAATAAACTAGAAGTTGGATTAGGAAGAGATATATCAGGTGATCCAATT
    ACTGTTCCACTAAATGAAATGCCACACTTATTGGTGGCAGGATCGACGGGTAGTGGTAAA
    TCTGTTTGTATAAATGGTATTATTACAAGTATTTTATTAAATGCTAAGCCGCATGAAGTT
    AAACTTATGTTAATCGATCCGAAAATGGTTGAACTAAATGTTTATAACGGAATTCCACAC
    TTATTAATTCCGGTTGTTACAAATCCTCATAAAGCTGCTCAAGCTTTAGAAAAAATTGTA
    GCTGAGATGGAAAGACGTTATGATTTATTCCAACATTCATCAACTAGAAACATTAAAGGT
    TATAACGAATTAATCCGTAAGCAAAATCAAGAATTAGATGAGAAGCAACCAGAATTACCT
    TATATCGTTGTTATTGTAGATGAGCTTGCAGATTTAATGATGGTAGCTGGTAAAGAAGTT
    GAAAATGCGATTCAACGTATTACACAAATGGCACGTGCAGCAGGTATACATTTAATTGTA
    GCGACACAAAGACCTTCTGTGGATGTAATTACAGGTATCATTAAAAATAATATTCCATCT
    AGAATAGCTTTTGCTGTGAGTTCTCAAACAGATTCAAGAACTATTATTGGTACTGGCGGC
    GCAGAAAAGTTACTTGGTAAAGGTGACATGTTATACGTTGGAAATGGTGACTCATCACAA
    ACACGTATTCAAGGGGCGTTTTTAAGTGACCAAGAGGTGCAAGATGTTGTAAATTATGTA
    GTAGAACAACAACAGGCAAATTATGTAAAAGAAATGGAACCAGATGCACCAGTGGATAAA
    TCGGAAATGAAAAGTGAAGATGCTTTATATGATGAAGCGTATTTGTTTGTTGTTGAACAA
    CAAAAGGCAAGTACATCATTGTTACAACGCCAATTTAGAATTGGTTATAATAGAGCATCT
    AGGTTGATGGATGATTTAGAACGCAATCAGGTAATCGGTCCACAAAAAGGAAGCAAGCCT
    AGACAAGTTTTAATAGATCTTAATAATGACGAGGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2379

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1295 [new locus tag: SACOL_RS06610 ]
  • symbol: SACOL1295
  • description: FtsK/SpoIIIE family protein
  • length: 792
  • theoretical pI: 9.16869
  • theoretical MW: 88570
  • GRAVY: -0.298864

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EssC (TIGR03928; HMM-score: 123.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EccCa (TIGR03924; HMM-score: 103)
    and 4 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EccCb (TIGR03925; HMM-score: 64.9)
    Genetic information processing Mobile and extrachromosomal element functions Other conjugative coupling factor TraD, SXT/TOL subfamily (TIGR03743; HMM-score: 14.7)
    type IV conjugative transfer system coupling protein TraD (TIGR02759; HMM-score: 12.3)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation transporter family protein (TIGR00860; HMM-score: 11.3)
  • TheSEED  :
    • Cell division protein FtsK
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Cell division protein FtsK
  • PFAM:
    P-loop_NTPase (CL0023) FtsK_SpoIIIE; FtsK/SpoIIIE family (PF01580; HMM-score: 205.4)
    and 15 more
    HTH (CL0123) Ftsk_gamma; Ftsk gamma domain (PF09397; HMM-score: 100.6)
    P-loop_NTPase (CL0023) AAA_10; AAA-like domain (PF12846; HMM-score: 26.4)
    no clan defined FtsK_4TM; 4TM region of DNA translocase FtsK/SpoIIIE (PF13491; HMM-score: 21.1)
    P-loop_NTPase (CL0023) AAA_19; AAA domain (PF13245; HMM-score: 20.7)
    DUF87; Domain of unknown function DUF87 (PF01935; HMM-score: 20.3)
    AAA_22; AAA domain (PF13401; HMM-score: 16.9)
    T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 15.4)
    TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 14.8)
    Marvel-like (CL0396) MARVEL; Membrane-associating domain (PF01284; HMM-score: 14.7)
    P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 14)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13.4)
    no clan defined CrgA; Cell division protein CrgA (PF06781; HMM-score: 13.2)
    Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region (PF02932; HMM-score: 12.3)
    ClpP_crotonase (CL0127) Peptidase_S49_N; Peptidase family S49 N-terminal (PF08496; HMM-score: 11.1)
    no clan defined Codanin-1_C; Codanin-1 C-terminus (PF15296; HMM-score: 10.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008695
    • TAT(Tat/SPI): 0.00019
    • LIPO(Sec/SPII): 0.001373
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRQLAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMVLGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQIALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLISLFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKREEKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFDAENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAKQKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLHNDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGRDISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVELNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQNQELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSVDVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQGAFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKASTSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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