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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1302 [new locus tag: SACOL_RS06645 ]
  • pan locus tag?: SAUPAN003585000
  • symbol: pgsA
  • pan gene symbol?: pgsA
  • synonym:
  • product: CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1302 [new locus tag: SACOL_RS06645 ]
  • symbol: pgsA
  • product: CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1317876..1318454
  • length: 579
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAATATTCCGAACCAGATTACGGTTTTTAGAGTAGTGTTAATACCAGTTTTTATATTG
    TTTGCGTTAGTTGATTTTGGATTTGGCAATGTGTCATTTCTAGGAGGATATGAAATAAGA
    ATTGAGTTATTAATCAGTGGTTTTATTTTTATATTGGCTTCCCTTAGCGATTTTGTTGAT
    GGTTATTTAGCTAGAAAATGGAATTTAGTTACAAATATGGGGAAATTTTTGGATCCATTA
    GCGGATAAATTATTAGTTGCAAGTGCTTTAATTGTACTTGTGCAACTAGGACTAACAAAT
    TCTGTAGTAGCAATCATTATTATTGCCAGAGAATTTGCCGTAACTGGTTTACGTTTACTA
    CAAATTGAACAAGGATTCGTAAGTGCAGCTGGTCAATTAGGTAAAATTAAAACAGCAGTT
    ACTATGGTAGCAATTACTTGGTTGTTATTAGGTGATCCATTGGCAACATTGATTGGTTTG
    TCATTAGGACAAATTTTATTATACATTGGCGTTATTTTTACTATCTTATCTGGTATTGAA
    TACTTTTATAAAGGTAGAGATGTTTTTAAACAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    579

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1302 [new locus tag: SACOL_RS06645 ]
  • symbol: PgsA
  • description: CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
  • length: 192
  • theoretical pI: 9.39765
  • theoretical MW: 21014.1
  • GRAVY: 1.02448

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.8.5?  ExPASy
    CDP-diacylglycerol--glycerol-3-phosphate 1-phosphatidyltransferase CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate
  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (TIGR00560; EC 2.7.8.5; HMM-score: 191.2)
    and 1 more
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis CDP-diacylglycerol-serine O-phosphatidyltransferase (TIGR00473; EC 2.7.8.8; HMM-score: 33.4)
  • TheSEED  :
    • CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5)
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5)
  • PFAM:
    no clan defined CDP-OH_P_transf; CDP-alcohol phosphatidyltransferase (PF01066; HMM-score: 60.1)
    and 1 more
    Holin-II (CL0563) Phage_holin_2_3; Bacteriophage holin family HP1 (PF16080; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.019369
    • TAT(Tat/SPI): 0.000171
    • LIPO(Sec/SPII): 0.003491
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELLISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSVVAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGLSLGQILLYIGVIFTILSGIEYFYKGRDVFKQK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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