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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1197 [new locus tag: SACOL_RS06130 ]
  • pan locus tag?: SAUPAN003454000
  • symbol: divIB
  • pan gene symbol?: divIB
  • synonym:
  • product: cell division protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1197 [new locus tag: SACOL_RS06130 ]
  • symbol: divIB
  • product: cell division protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1202498..1203817
  • length: 1320
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGATAAAACGAAGAACGATCAACAAGAATCAAATGAAGATAAAGATGAATTAGAA
    TTATTTACGAGGAATACATCTAAGAAAAGACGGCAAAGAAAAAGATCAAAGGCTACACAT
    TTTTCTAATCAAAATAAAGATGATACATCTCAACAAGCTGATTTTGATGAAGAAATTTAC
    TTGATAAATAAAGACTTCAAAAAAGAAGAAAGCAATGATAAAAATAATGATTCTGCTTCT
    AGTCATGCGAATGATAATAATATCGATGATTCTACAGACTCTAATATTGAAAATGAGGAT
    TATAGATATAATCAAGAAATTGACGACCAAAATGAATCGAATGTAATTTCAGTCGACAAC
    GAACAACCTCAATCAGCTCCTAAAGAACAAAATAGCGACTCGATTGATGAGGAAACAGTA
    ACGAAAAAAGAACGAAAAAGTAAAGTAACACAATTAAAGCCATTAACACTTGAAGAAAAG
    CGGAAGTTAAGACGTAAGCGACAAAAGCGAATCCAATACAGTGTTATTACAATATTGGTA
    TTGTTGATTGCTGTTATATTAATTTACATGTTTTCACCACTTAGTAAAATTGCGCATGTA
    AATATAAATGGAAATAATCACGTTAGTACTTCAAAGATAAACAAAGTTTTAGGTGTTAAA
    AATGATTCAAGGATGTATACGTTTAGTAAAAAAAATGCTATTAATGATCTCGAAGAGAAT
    CCATTAATCAAAAGTGTTGAGATACACAAGCAATTACCAAACACATTAAACGTAGATATC
    ACAGAAAATGAAATTATTGCTTTAGTGAAATATAAAGGTAAATATTTACCTTTATTAGAA
    AATGGTAAATTGCTTAAAGGTTCAAATGATGTCAAAATTAATGATGCACCTGTCATGGAT
    GGTTTCAAAGGTACAAAAGAAGATGATATGATTAAGGCGTTATCTGAAATGACACCTGAA
    GTTAGACGATATATTGCCGAAGTGACATACGCCCCAAGTAAAAACAAACAAAGCAGAATT
    GAATTGTTTACGACAGATGGACTTCAAGTAATCGGTGATATTTCGACGATATCTAAGAAA
    ATGAAATATTATCCGCAGATGTCACAATCATTATCAAGGGATAGTTCGGGTAAACTAAAA
    ACACGAGGCTATATTGATTTATCAGTCGGTGCTTCATTTATCCCATACCGTGGAAACACG
    TCTAGTCAATCAGAAAGCGATAAAAATGTGACTAAATCATCTCAAGAGGAAAATCAAGCA
    AAAGAAGAATTACAAAGCGTTTTAAACAAAATTAACAAACAATCAAGTAAGAATAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1197 [new locus tag: SACOL_RS06130 ]
  • symbol: DivIB
  • description: cell division protein
  • length: 439
  • theoretical pI: 8.50233
  • theoretical MW: 50211.6
  • GRAVY: -1.03804

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides LPS export ABC transporter permease LptF (TIGR04407; HMM-score: 12.9)
    Metabolism Transport and binding proteins Other LPS export ABC transporter permease LptF (TIGR04407; HMM-score: 12.9)
  • TheSEED  :
    • Cell division protein FtsQ
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Cell division protein FtsQ
  • PFAM:
    POTRA (CL0191) POTRA_1; POTRA domain, FtsQ-type (PF08478; HMM-score: 66.9)
    and 5 more
    POTRA; Surface antigen variable number repeat (PF07244; HMM-score: 17.5)
    no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 13.2)
    BPD_transp_1 (CL0404) YjgP_YjgQ; Predicted permease YjgP/YjgQ family (PF03739; HMM-score: 11.9)
    no clan defined DUF5316; Family of unknown function (DUF5316) (PF17247; HMM-score: 11.2)
    T2SSF; Type II secretion system (T2SS), protein F (PF00482; HMM-score: 11)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.53
    • Cellwall Score: 8.75
    • Extracellular Score: 0.7
    • Internal Helix: 1
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01083
    • TAT(Tat/SPI): 0.007453
    • LIPO(Sec/SPII): 0.004758
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQNKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYRYNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEKRKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLGVKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYLPLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSKNKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGASFIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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