PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1027 [new locus tag: SA_RS05850 ]
• pan locus tag^?: SAUPAN003454000
• symbol: div1b
• pan gene symbol^?: divIB
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1027 [new locus tag: SA_RS05850 ]
• symbol: div1b
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1162912..1164231
• length: 1320
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123858 NCBI
• RefSeq: NP_374300 NCBI
• BioCyc: see SA_RS05850
• MicrobesOnline: 103326 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGGATGATAAAACGAAGAACGATCAACAAGAATCAAATGAAGATAAAGATGAATTAGAA
  TTATTTACGAGGAATACATCTAAGAAAAGACGGCAAAGAAAAAGGTCAAAGGCTACACAT
  TTTTCTAATCAAAATAAAGATGATACATCTCAACAAGCTGATTTTGATGAAGAAATTTAC
  TTGATAAATAAAGACTTCAAAAAAGAAGAAAGCAATGATGAAAATAATGGTTCTGCTTCT
  AGTCATGCGAATGATAATAATATCGATGATTCTACAGACTCTAATATTGAAAATGAGGAT
  TATAGATATAATCAAGAAATTGACGACCAAAATGAATCGAATGGAATTGCAGTCGGCAAC
  GAACAACCTCAATCAGCTCCTAAAGAACAAAATAGCGACTCGAATGATGAGGAAACAGTA
  ACGAAAAAAGAACGAAAAAGTAAAGTAACACAATTAAAGCCATTAACACTTGAAGAAAAG
  CGGAAGTTAAGACGTAAGCGACAAAAACGAATCCAATACAGTGTTATTACAATATTAGTA
  TTGTTGATTGCTGTTATATTAATTTACATGTTTTCACCACTTAGTAAAATTGCGCATGTA
  AATATAAATGGAAATAATCACGTTAGTACTTCAAAGATAAACAAAGTTTTAGGTGTTAAA
  AATGATTCGAGGATGTATACGTTTAGTAAAAAAAATGCTATTAATGATCTCGAAGAGGAT
  CCATTAATCAAAAGTGTTGAGATACACAAGCAATTACCAAACACATTAAACGTAGATATC
  ACAGAAAATGAAATTATTGCTTTAGTGAAATATAAAGGTAAATATTTACCTTTATTAGAA
  AATGGTAAATTGCTTAAAGGTTCAAATGATGTCAAAATTAATGATGCACCTGTCATGGAT
  GGTTTCAAAGGTACAAAAGAAGATGATATGATTAAGGCGTTATCTGAAATGACACCTGAA
  GTTAGACGATATATTGCCGAAGTGACATACGCCCCAAGTAAAAACAAACAAAGCAGAATT
  GAATTGTTTACGACAGATGGACTTCAAGTAATCGGTGATATTTCGACGATATCTAAGAAA
  ATGAAATATTATCCGCAGATGTCACAATCATTATCAAGGGATAGTTCGGGTAAACTAAAA
  ACAAGAGGCTATATTGATTTATCAGTCGGTGCTTCATTTATCCCATACCGTGGAAACACG
  TCTAGTCAATCAGAAAGCGATAAAAATGTGACTAAATCATCTCAAGAGGAAAATCAAGCA
  AAAGAAGAATTACAAAGCGTTTTAAACAAAATTAACAAACAATCAAGTAAGAATAATTAA
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⊟Protein

⊟General

• locus tag: SA1027 [new locus tag: SA_RS05850 ]
• symbol: Div1b
• description: hypothetical protein
• length: 439
• theoretical pI: 7.79288
• theoretical MW: 50040.3
• GRAVY: -1.04579

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides LPS export ABC transporter permease LptF (TIGR04407; HMM-score: 11.6)
  Metabolism Transport and binding proteins Other LPS export ABC transporter permease LptF (TIGR04407; HMM-score: 11.6)
  and 1 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds K+-dependent Na+/Ca+ exchanger (TIGR00927; HMM-score: 3.3)
• TheSEED  :
  • Cell division protein FtsQ
  Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Cell division protein FtsQ
• PFAM:
  POTRA (CL0191) POTRA_1; POTRA domain, FtsQ-type (PF08478; HMM-score: 67)
  and 7 more
  POTRA; Surface antigen variable number repeat (PF07244; HMM-score: 16.1)
  BPD_transp_1 (CL0404) YjgP_YjgQ; Predicted permease YjgP/YjgQ family (PF03739; HMM-score: 14.9)
  no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 13.1)
  Coiled-coil_56; Coiled-coil domain-containing protein 56 (PF09813; HMM-score: 12.7)
  VSA (CL0656) RIFIN; Rifin (PF02009; HMM-score: 11.7)
  no clan defined DUF5316; Family of unknown function (DUF5316) (PF17247; HMM-score: 11.3)
  T2SSF; Type II secretion system (T2SS), protein F (PF00482; HMM-score: 10.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 0.53
  • Cellwall Score: 8.75
  • Extracellular Score: 0.7
  • Internal Helix: 1
• LocateP: Intracellular /TMH start AFTER 60
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: Possibly Sec-
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01083
  • TAT(Tat/SPI): 0.007453
  • LIPO(Sec/SPII): 0.004758
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 15926767 NCBI
• RefSeq: NP_374300 NCBI
• UniProt: A0A0H3JKR6 UniProt

⊟Protein sequence

• MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQNKDDTSQQADFDEEIYLINKDFKKEESNDENNGSASSHANDNNIDDSTDSNIENEDYRYNQEIDDQNESNGIAVGNEQPQSAPKEQNSDSNDEETVTKKERKSKVTQLKPLTLEEKRKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLGVKNDSRMYTFSKKNAINDLEEDPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYLPLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSKNKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGASFIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: mraY > murD > div1b > ftsA > ftsZ

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications