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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1027 [new locus tag: SA_RS05850 ]
  • pan locus tag?: SAUPAN003454000
  • symbol: div1b
  • pan gene symbol?: divIB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1027 [new locus tag: SA_RS05850 ]
  • symbol: div1b
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1162912..1164231
  • length: 1320
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGATAAAACGAAGAACGATCAACAAGAATCAAATGAAGATAAAGATGAATTAGAA
    TTATTTACGAGGAATACATCTAAGAAAAGACGGCAAAGAAAAAGGTCAAAGGCTACACAT
    TTTTCTAATCAAAATAAAGATGATACATCTCAACAAGCTGATTTTGATGAAGAAATTTAC
    TTGATAAATAAAGACTTCAAAAAAGAAGAAAGCAATGATGAAAATAATGGTTCTGCTTCT
    AGTCATGCGAATGATAATAATATCGATGATTCTACAGACTCTAATATTGAAAATGAGGAT
    TATAGATATAATCAAGAAATTGACGACCAAAATGAATCGAATGGAATTGCAGTCGGCAAC
    GAACAACCTCAATCAGCTCCTAAAGAACAAAATAGCGACTCGAATGATGAGGAAACAGTA
    ACGAAAAAAGAACGAAAAAGTAAAGTAACACAATTAAAGCCATTAACACTTGAAGAAAAG
    CGGAAGTTAAGACGTAAGCGACAAAAACGAATCCAATACAGTGTTATTACAATATTAGTA
    TTGTTGATTGCTGTTATATTAATTTACATGTTTTCACCACTTAGTAAAATTGCGCATGTA
    AATATAAATGGAAATAATCACGTTAGTACTTCAAAGATAAACAAAGTTTTAGGTGTTAAA
    AATGATTCGAGGATGTATACGTTTAGTAAAAAAAATGCTATTAATGATCTCGAAGAGGAT
    CCATTAATCAAAAGTGTTGAGATACACAAGCAATTACCAAACACATTAAACGTAGATATC
    ACAGAAAATGAAATTATTGCTTTAGTGAAATATAAAGGTAAATATTTACCTTTATTAGAA
    AATGGTAAATTGCTTAAAGGTTCAAATGATGTCAAAATTAATGATGCACCTGTCATGGAT
    GGTTTCAAAGGTACAAAAGAAGATGATATGATTAAGGCGTTATCTGAAATGACACCTGAA
    GTTAGACGATATATTGCCGAAGTGACATACGCCCCAAGTAAAAACAAACAAAGCAGAATT
    GAATTGTTTACGACAGATGGACTTCAAGTAATCGGTGATATTTCGACGATATCTAAGAAA
    ATGAAATATTATCCGCAGATGTCACAATCATTATCAAGGGATAGTTCGGGTAAACTAAAA
    ACAAGAGGCTATATTGATTTATCAGTCGGTGCTTCATTTATCCCATACCGTGGAAACACG
    TCTAGTCAATCAGAAAGCGATAAAAATGTGACTAAATCATCTCAAGAGGAAAATCAAGCA
    AAAGAAGAATTACAAAGCGTTTTAAACAAAATTAACAAACAATCAAGTAAGAATAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1027 [new locus tag: SA_RS05850 ]
  • symbol: Div1b
  • description: hypothetical protein
  • length: 439
  • theoretical pI: 7.79288
  • theoretical MW: 50040.3
  • GRAVY: -1.04579

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides LPS export ABC transporter permease LptF (TIGR04407; HMM-score: 11.6)
    Metabolism Transport and binding proteins Other LPS export ABC transporter permease LptF (TIGR04407; HMM-score: 11.6)
    and 1 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds K+-dependent Na+/Ca+ exchanger (TIGR00927; HMM-score: 3.3)
  • TheSEED  :
    • Cell division protein FtsQ
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Cell division protein FtsQ
  • PFAM:
    POTRA (CL0191) POTRA_1; POTRA domain, FtsQ-type (PF08478; HMM-score: 68.8)
    and 6 more
    no clan defined FtsQ_DivIB_C; Cell division protein FtsQ/DivIB, C-terminal (PF03799; HMM-score: 49.4)
    POTRA (CL0191) POTRA; Surface antigen variable number repeat (PF07244; HMM-score: 16.5)
    BPD_transp_1 (CL0404) LptF_LptG; Lipopolysaccharide export system permease LptF/LptG (PF03739; HMM-score: 13.7)
    no clan defined T2SSF; Type II secretion system (T2SS), protein F (PF00482; HMM-score: 12.5)
    DUF6480; Family of unknown function (DUF6480) (PF20088; HMM-score: 11.4)
    VSA (CL0656) RIFIN; Rifin (PF02009; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.53
    • Cellwall Score: 8.75
    • Extracellular Score: 0.7
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9926
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0073
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01083
    • TAT(Tat/SPI): 0.007453
    • LIPO(Sec/SPII): 0.004758
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQNKDDTSQQADFDEEIYLINKDFKKEESNDENNGSASSHANDNNIDDSTDSNIENEDYRYNQEIDDQNESNGIAVGNEQPQSAPKEQNSDSNDEETVTKKERKSKVTQLKPLTLEEKRKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLGVKNDSRMYTFSKKNAINDLEEDPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYLPLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSKNKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGASFIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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