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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0487 [new locus tag: SACOL_RS02465 ]
  • pan locus tag?: SAUPAN002146000
  • symbol: SACOL0487
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0487 [new locus tag: SACOL_RS02465 ]
  • symbol: SACOL0487
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 488401..489726
  • length: 1326
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    TTGAGTAGGAAGTATAAAATAGATTTAAAAGCAATTAATCAAAATACTGAGTCTACAAGT
    GCTATTTCGAAAGCGTCATACGAAGTTGAGAATGCTAATAATAATGGTCTATCAAAAAGA
    GACGTGATTAATCAATTTAATGATTTGAAAAAAATGAAAAAATTCCCTTCTAATCTTGAA
    TATGTTGATAGTTACACTGATTCTCTTACTGGAGTAACAACTTCTGCTTTTTTAAATAAA
    GATACAGGCAAAGTAACTCTCGGGATGACTGGGACTAATTTACAAGACGAAGCCTTTAAA
    AAGTTAAAAGAAGGTGAATTTTCAAGACAAAATGTTACCAATGCTTTGGAAACAGTTAAA
    GATGGATATGCAGATCTTAAAATATTATATTCTCCTGCATCTGATCAAAACTATAGATAT
    GCGAATACACAAGAATTTATAAATAAAATAAAAAGTAAGTATGACATTGATTTTATTACT
    GGACATTCACTAGGTGGAAGAGATGCGGTAGTTCTAGGAATGAGTAATGGTATTCCGAAC
    ATTGTGGTTTATAATCCAGCTCCTATTTCTATAACTAGTTTGAATCCTAATTCCCCAGAT
    GGAAAACGTTTATTAGAATTATATAAAAATTATAAAGGTAATATTACTAGGTTTGTTGCA
    GAAAATGATGCATTGACAGAAAATCTGAAGAAATATAAGCATTATGTTTTTTTCGGTAAT
    GATAAAGTCTTTAAAAATGGTAAAGGTCATGAAATGGAAGGCTTTCTGACCGAAGAAGAA
    CAAAAAGCTATAAAAAAAGAACTTAAAAAACTACAAGGTTATGCAGAAGAAAATAATAAG
    TCATTTGTAAAGAATTCAAATAATGCTATCTCTAAATTAGCTAGTATAGAATTACTTAGA
    GCAAATATGATGACTACAAATGGAGGAGGATTATCTTCTTCGCAGCAAAAAGTTTTAGAA
    AGTTTAACAGCTTTAACAATTGCGCAGTCATTCAGTCAACTGATAGACGATGAAATTAAT
    CAAATCAAAAAAATGTATAATGAAAAGAAAAAGAAATTTGGAAAAAATTGGGAAGACGCT
    CAAAAAGCTGGAAAAGCTGTAGGTGAAGATTTGAGTGTAAATGGAGTTCTCAATGCTTTA
    GATGAAGGTCAAGTGAATGAAAGTAGTATGGTAAGAGAACCTGAACAAATGATATCTGCA
    AAAGAAAGACAACTTTCAACGATAGGGTCTTCTGTATCAAATTATATTATGAGAGTTAGA
    CTCAGTATTAATGAAATCGTTGATAAAGATCAAGTGCTTGCATCACAAATAGGTGGATTA
    CTATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1326

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0487 [new locus tag: SACOL_RS02465 ]
  • symbol: SACOL0487
  • description: hypothetical protein
  • length: 441
  • theoretical pI: 9.34275
  • theoretical MW: 49148.2
  • GRAVY: -0.602494

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Degradation esterase, PHB depolymerase family (TIGR01840; EC 3.1.1.-; HMM-score: 11.4)
  • TheSEED  :
    • FIG01107856: hypothetical protein
  • PFAM:
    AB_hydrolase (CL0028) Lipase_3; Lipase (class 3) (PF01764; HMM-score: 15.1)
    RXXXE-like (CL0797) DUF5677; Family of unknown function (DUF5677) (PF18928; HMM-score: 12.3)
    and 2 more
    no clan defined Erf4; Golgin subfamily A member 7/ERF4 family (PF10256; HMM-score: 11.6)
    Apolipoprotein (CL0725) Apo-CIII; Apolipoprotein CIII (Apo-CIII) (PF05778; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0003
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.9992
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.020672
    • TAT(Tat/SPI): 0.003055
    • LIPO(Sec/SPII): 0.003521
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVINQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKKLKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFITGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITRFVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYAEENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQLIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMVREPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 71 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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