From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2189 [new locus tag: SA_RS12555 ]
  • pan locus tag?: SAUPAN005933000
  • symbol: SA2189
  • pan gene symbol?: nirR
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2189 [new locus tag: SA_RS12555 ]
  • symbol: SA2189
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2460468..2461199
  • length: 732
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    GTGAATGGGAATATCATTGTTGCACATGGCATGAGGCACGGACGACAGAATCAAGCGTTA
    GAAGCATTTATTTCTGAATTAGTAAAAGATGATATACATCACTATGATATTGCGTTTTTA
    GAAAGTGAGCATCAGGATTTAGAGACGGTAATGACGACGTTGATTCAAAGTGGGGTTGAC
    CATTTTAAAATTGTACCATTGCTAATCTTTAGTGCAATGCATTATCTCAAGGATATACCG
    AATATCGTTCATGAAATGAAACGACGGTATCCAGACATAAAGGTAGAAGTAAGCGAGCCA
    CTTGGCACACATCCTTTAATGAGACGATTAATTGAACAACGTATTTGTGACGCATTAGCA
    GGTGACGAACAACAAGTTGGAGTTATGGTTGTTGCACACGGCAATATTAATGGAAATTTT
    ACTAAGGCACACGAAGAGTTACAAATATGCGCTCAACATTTGCAGATTAATAAACCTACT
    TATGCGAGAACGCTATATGGAGAGATTAGTTTTACACATGATTTAGAAAGCATCTCGAAA
    CGGTATCAAAAATTGATTGTCGTACCATTATTTTTATATGACGGAAGACTTGTTAACAAA
    GTGAAGCAGCAAATGAATGACATGGTTATTAATACAGATATACACTTCACACCTTCAATT
    AACTTCGACCCAATTTTAAAGCAAATCATTAACGATAGATTAGAAAGCTTAATGATTCCG
    ATGAAAATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    732

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2189 [new locus tag: SA_RS12555 ]
  • symbol: SA2189
  • description: hypothetical protein
  • length: 243
  • theoretical pI: 6.62908
  • theoretical MW: 27984.3
  • GRAVY: -0.155556

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Sirohydrochlorin ferrochelatase (EC 4.99.1.4)
    Experimental tye  Sirohydrochlorin ferrochelatase (EC 4.99.1.4)
  • PFAM:
    Chelatase (CL0043) CbiX; CbiX (PF01903; HMM-score: 116)
    and 2 more
    CbiK; Cobalt chelatase (CbiK) (PF06180; HMM-score: 22.6)
    no clan defined DUF3928; Protein of unknown function (DUF3928) (PF13065; HMM-score: 14.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002001
    • TAT(Tat/SPI): 0.000103
    • LIPO(Sec/SPII): 0.000434
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEHQDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLGTHPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGNFTKAHEELQICAQHLQINKPTYARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTPSINFDPILKQIINDRLESLMIPMKI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, NreC* (activation) regulon
    Rex*(TF)important in Energy metabolism; RegPrecise 
    NreC*(TF)important in Nitrate and nitrite respiration; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA2189&oldid=96291"