PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2186 [new locus tag: SA_RS12540 ]
• pan locus tag^?: SAUPAN005930000
• symbol: nasF
• pan gene symbol^?: nasF
• synonym:
• product: uroporphyrin-III C-methyl transferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2186 [new locus tag: SA_RS12540 ]
• symbol: nasF
• product: uroporphyrin-III C-methyl transferase
• replicon: chromosome
• strand: -
• coordinates: 2456711..2457688
• length: 978
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125114 NCBI
• RefSeq: NP_375509 NCBI
• BioCyc: see SA_RS12540
• MicrobesOnline: 104535 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTCTGTAGATAAATATGGCAAGGTTTACTTGATAGGTGCGGGACCAGGTAATCCGAAT
  TATTTGACTAAAAAGGCTGAGCGTTTAATACGTGAAGCGGATGTTATACTATATGATCGA
  TTAGTGAATCCGTTAATTTTACAATATGCGAATTTGACTACTGAAATTATCGATGTTGGT
  AAAAAGCCGTATGCAAAACACATTCAGCAAGAAAAAATAAATGAATGTATCGTGGAAGCA
  GCACGTCGATATAACAAGGTTGTGAGGCTTAAAGGTGGCGACCCAGCAATATTTGGACGA
  GTTCAAGAAGAAGTTGACACACTAAACAACTTTAATATTGCGTTTGAAATTGTACCTGGT
  GTGACATCTGCGAGTGCTGCAGTTGCTACGATGCAAACAGGATTAACGATGCGTGCTGTT
  GCTAAGAGTGTGACATTTTCTACAGGTCACTTTAAAGATTCAGAAGAAAATGAAGTAGAT
  GTCAACGCTTTAGTAAATGGTGGTACGTTGGCGATATATATGGGAGTTAAACGTTTAGGG
  AAAATTATCGCCCAGATACAACAGTACACAGATATTGATTATCCGATAGCGATTGTCTTT
  CAAGCATCGTGTTTTAACGAGTTTGTTGTTAAGGGGCGTTTAAGTAATATTGTTGGGAAA
  CTGGAGCATTACGCAATTGAGGCGAAACCAGGTATATGTATTATAGGCGAAGTAGTGGGT
  TATACTGAAAATGTCTCAACAACTTCTAATCCGACTCAGCAATTTTATGTAGTAAGTGGT
  TCTAGACATGACGCCCTTATGCTCTGTGAACATTTATATGACGAAGGTTATGGCTGTTTG
  CTAAACCCAAATGATACATCGAATGGCACATATCATTCGTCGCAATATGATTACTATGAT
  GCATTTATTAAGCAGCAAGAAAATGTAACATATATTTCAACCGATCGTGCAGATGCTAAT
  ACAGTGTTATGTCACTAA
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⊟Protein

⊟General

• locus tag: SA2186 [new locus tag: SA_RS12540 ]
• symbol: NasF
• description: uroporphyrin-III C-methyl transferase
• length: 325
• theoretical pI: 5.64381
• theoretical MW: 36008.6
• GRAVY: -0.159385

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin uroporphyrinogen-III C-methyltransferase (TIGR01469; EC 2.1.1.107; HMM-score: 292.4)
  and 5 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-4 C11-methyltransferase (TIGR01465; EC 2.1.1.133; HMM-score: 154.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-3B C17-methyltransferase (TIGR01466; EC 2.1.1.131; HMM-score: 46.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-2 C(20)-methyltransferase (TIGR01467; EC 2.1.1.130; HMM-score: 44.9)
  precorrin-6A synthase (deacetylating) (TIGR02434; EC 2.1.1.152; HMM-score: 40.6)
  precorrin-6y C5,15-methyltransferase (decarboxylating), CbiE subunit (TIGR02467; EC 2.1.1.132; HMM-score: 38.6)
• TheSEED  :
  • Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Tetrapyrroles CPO analysis  Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)
• PFAM:
  no clan defined TP_methylase; Tetrapyrrole (Corrin/Porphyrin) Methylases (PF00590; HMM-score: 154.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003085
  • TAT(Tat/SPI): 0.000116
  • LIPO(Sec/SPII): 0.000573
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927976 NCBI
• RefSeq: NP_375509 NCBI
• UniProt: A0A0H3JRK3 UniProt

⊟Protein sequence

• MSVDKYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNPLILQYANLTTEIIDVGKKPYAKHIQQEKINECIVEAARRYNKVVRLKGGDPAIFGRVQEEVDTLNNFNIAFEIVPGVTSASAAVATMQTGLTMRAVAKSVTFSTGHFKDSEENEVDVNALVNGGTLAIYMGVKRLGKIIAQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIVGKLEHYAIEAKPGICIIGEVVGYTENVSTTSNPTQQFYVVSGSRHDALMLCEHLYDEGYGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: nasF < nasE < nasD < SA2189

⊟Regulation

• regulators: Rex* (repression) regulon, NreC* (activation) regulon

  Rex*	(TF)	important in Energy metabolism; RegPrecise
  NreC*	(TF)	important in Nitrate and nitrite respiration; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications