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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002366
  • pan locus tag?: SAUPAN005930000
  • symbol: cobA
  • pan gene symbol?: nasF
  • synonym:
  • product: uroporphyrinogen-III C-methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002366
  • symbol: cobA
  • product: uroporphyrinogen-III C-methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2366962..2367939
  • length: 978
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCTGTAGAGGAATATGGCAAGGTTTACTTGATAGGTGCGGGCCCAGGTAATCCGAAT
    TATTTGACGAAAAAGGCTGAGCGTTTAATACGTGAAGCGGATGTCATTCTATATGATCGA
    TTAGTGAATCCGTTAATTTTACAATATGCGAATTTGACTACTGAAATTATCGATGTTGGT
    AAAAAGCCGTATGCAAAACATATTCAGCAAGAAAAAATAAATGACTGTATCGTGGAAGCA
    GCGCGTCGATATAACAAGGTTGTTAGACTTAAAGGTGGCGATCCAGCGATATTTGGTCGT
    GTGCAAGAAGAAGTCGACACACTAAATGAACATCATATTGCGTTTGAAATTGTACCAGGG
    GTGACATCAGCGAGCGCAGCAGTTGCTACTATGCAGACAGGTTTGACAATGCGTACAGTT
    GCTAAAAGTGTGACATTTTCTACAGGTCACTTTAAAGATTCAGAAGAAAATGAAGTGGAT
    GTCAATTCCTTAGTGAATGGTGGCACGTTAGCAATTTATATGGGGGTAAAACGTTTAGGG
    AAAATTATCACACAGATACAACAATACACAGATATTGATTACCCGATAGCGATTGTCTTT
    CAAGCATCGTGTTTTAACGAGTTTGTTGTTAAGGGGCGTTTAAGTAATATTATTTCAAAA
    TTGCAACACTATTCAATTGAGGCGAAACCAGGTATATGTATTATTGGGGAAGTTGTTGGT
    TATACTGAAAACACTCCTAAATCATATGATCCTATGAAGCAATTTTATGTAGTAAGTGGT
    GCTAAACATGACGCCCTTATGCTCTGTGAACATTTATATGACGAAGGTTATGGCTGTTTG
    CTAAACCCAAATGATACATCGAATGGCACATATCATTCGTCGCAATATGATTACTATGAT
    GCATTTATAAAGCAGCAAGAAAATGTAACATATATTTCAACCGATCGTGCAGATGCTAAT
    ACAGTGTTATGTCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    978

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002366
  • symbol: CobA
  • description: uroporphyrinogen-III C-methyltransferase
  • length: 325
  • theoretical pI: 5.82943
  • theoretical MW: 36260.9
  • GRAVY: -0.22

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.107?  ExPASy
    Uroporphyrinogen-III C-methyltransferase 2 S-adenosyl-L-methionine + uroporphyrinogen III = 2 S-adenosyl-L-homocysteine + precorrin-2
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin uroporphyrinogen-III C-methyltransferase (TIGR01469; EC 2.1.1.107; HMM-score: 296.8)
    and 6 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-4 C11-methyltransferase (TIGR01465; EC 2.1.1.133; HMM-score: 155.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-3B C17-methyltransferase (TIGR01466; EC 2.1.1.131; HMM-score: 47.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-2 C(20)-methyltransferase (TIGR01467; EC 2.1.1.130; HMM-score: 45)
    precorrin-6A synthase (deacetylating) (TIGR02434; EC 2.1.1.152; HMM-score: 40.7)
    precorrin-6y C5,15-methyltransferase (decarboxylating), CbiE subunit (TIGR02467; EC 2.1.1.132; HMM-score: 39.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE (TIGR03365; HMM-score: 12.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined TP_methylase; Tetrapyrrole (Corrin/Porphyrin) Methylases (PF00590; HMM-score: 176.6)
    and 2 more
    IKBKB_SDD; IQBAL scaffold dimerization domain (PF18397; HMM-score: 16.3)
    DUF6200; Family of unknown function (DUF6200) (PF19702; HMM-score: 15.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9844
    • Cytoplasmic Membrane Score: 0.007
    • Cell wall & surface Score: 0.0008
    • Extracellular Score: 0.0078
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002926
    • TAT(Tat/SPI): 0.000133
    • LIPO(Sec/SPII): 0.001011
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNPLILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQEEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVDVNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIISKLQHYSIEAKPGICIIGEVVGYTENTPKSYDPMKQFYVVSGAKHDALMLCEHLYDEGYGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, ArcR* (activation) regulon, NreC (activation) regulon
    Rex*(TF)important in Energy metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    ArcR*(TF)important in Arginine degradation;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    NreC(TF)important in Nitrate and nitrite respiration;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 2.2 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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