From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1125 [new locus tag: SA_RS06360 ]
  • pan locus tag?: SAUPAN003584000
  • symbol: SA1125
  • pan gene symbol?: rodZ
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1125 [new locus tag: SA_RS06360 ]
  • symbol: SA1125
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1278026..1278418
  • length: 393
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    TTGAAAACGGTCGGTGAAGCGCTAAAAGGTAGACGTGAAAGGTTAGGAATGACTTTAACA
    GAATTAGAGCAACGTACTGGAATTAAACGTGAAATGCTAGTGCATATTGAAAATAATGAA
    TTCGATCAACTACCGAATAAAAATTACAGCGAAGGATTTATTAGAAAATATGCAAGCGTA
    GTAAATATTGAACCTAACCAATTAATTCAAGCTCATCAAGATGAAATTCCATCGAACCAA
    GCCGAATGGGACGAAGTAATTACAGTTTTCAATAATAATAAAGACTTAGATTATAAGAGT
    AAATCAAAAGAGCCAATACAATTATTAGTAATCATGGGTATTACAGTTTTAATAACTTTA
    TTGTTATGGATCATGTTAGTTTTAATATTTTAA
    60
    120
    180
    240
    300
    360
    393

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1125 [new locus tag: SA_RS06360 ]
  • symbol: SA1125
  • description: hypothetical protein
  • length: 130
  • theoretical pI: 5.24544
  • theoretical MW: 15113.4
  • GRAVY: -0.226154

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function General mobile mystery protein A (TIGR02612; HMM-score: 16.1)
  • TheSEED  :
    • Cytoskeleton protein RodZ
  • PFAM:
    HTH (CL0123) HTH_25; Helix-turn-helix domain (PF13413; HMM-score: 60.3)
    and 7 more
    HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 37.1)
    HTH_3; Helix-turn-helix (PF01381; HMM-score: 25.1)
    HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 24.1)
    Rubredoxin (CL0045) COX5B; Cytochrome c oxidase subunit Vb (PF01215; HMM-score: 14.9)
    no clan defined Tmemb_9; TMEM9 (PF05434; HMM-score: 13.6)
    HTH (CL0123) HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 13.6)
    Sulfolobus_pRN; Sulfolobus plasmid regulatory protein (PF05584; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00614
    • TAT(Tat/SPI): 0.000912
    • LIPO(Sec/SPII): 0.002503
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQLPNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKSKEPIQLLVIMGITVLITLLLWIMLVLIF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Ling Xu, Svetlana E Sedelnikova, Patrick J Baker, David W Rice
Cloning, purification and preliminary crystallographic analysis of a putative DNA-binding membrane protein, YmfM, from Staphylococcus aureus.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2008, 64(Pt 7);656-8
[PubMed:18607101] [WorldCat.org] [DOI] (I p)

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA1125&oldid=95281"