From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0708 [new locus tag: SA_RS04025 ]
  • pan locus tag?: SAUPAN002662000
  • symbol: secA
  • pan gene symbol?: secA
  • synonym:
  • product: preprotein translocase subunit SecA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0708 [new locus tag: SA_RS04025 ]
  • symbol: secA
  • product: preprotein translocase subunit SecA
  • replicon: chromosome
  • strand: +
  • coordinates: 806411..808942
  • length: 2532
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGATTTTTATCAAAAATTCTTGATGGCAATAATAAAGAAATTAAACAGTTAGGTAAA
    CTTGCTGATAAAGTAATCGCTTTAGAAGAAAAAACGGCAATTTTAACTGATGAAGAAATT
    CGTAATAAAACGAAACAATTCCAAACAGAATTAGCTGACATTGATAATGTCAAAAAGCAA
    AATGATTATTTAGATAAAATTTTACCAGAAGCATATGCACTTGTTAGAGAAGGCTCTAAA
    CGTGTATTCAATATGACACCATATAAAGTTCAAATTATGGGTGGTATTGCAATTCATAAA
    GGTGATATCGCTGAGATGAGAACAGGTGAAGGTAAAACATTAACAGCGACAATGCCAACA
    TACTTAAATGCATTAGCTGGTAGAGGTGTTCACGTTATTACAGTCAATGAATACTTATCA
    AGTGTTCAAAGTGAAGAAATGGCTGAGTTATATAACTTCTTAGGTTTGACTGTCGGATTA
    AACTTAAACAGTAAGACGACAGAAGAAAAACGTGAAGCATACGCACAAGACATTACTTAC
    AGTACTAATAATGAGCTAGGTTTTGATTACTTACGAGATAACATGGTGAATTATTCTGAA
    GATAGAGTAATGCGTCCATTACATTTTGCAATCATTGATGAGGTTGACTCAATTTTAATC
    GACGAGGCACGTACGCCATTAATTATTTCTGGTGAAGCTGAAAAGTCAACGTCACTTTAT
    ACACAAGCAAATGTTTTTGCGAAAATGTTAAAACAGGACGAAGATTATAAATACGATGAA
    AAAACGAAAGCCGTACATTTAACAGAACAAGGTGCGGATAAAGCTGAACGTATGTTCAAA
    GTTGAAAACTTATATGATGTACAAAATGTTGATGTTATTAGTCATATCAACACAGCTTTA
    CGTGCGCACGTTACATTACAACGTGACGTAGACTATATGGTTGTTGATGGCGAAGTACTA
    ATTGTCGATCAATTTACAGGACGTACAATGCCAGGCCGTCGTTTCTCAGAAGGTTTACAC
    CAAGCTATTGAAGCGAAGGAAGGCGTTCAAATTCAAAATGAATCTAAAACTATGGCGTCT
    ATTACATTCCAAAACTATTTCAGAATGTACAATAAACTTGCGGGTATGACAGGTACAGCT
    AAAACTGAAGAAGAAGAATTTAGAAATATTTATAACATGACAGTAACTCAAATTCCGACA
    AATAAACCTGTGCAACGTAACGATAAGTCTGATTTAATTTACATTAGCCAAAAAGGTAAA
    TTTGATGCAGTAGTAGAAGATGTTGTTGAAAAACACAAGGCAGGGCAACCAGTGCTATTA
    GGTACTGTTGCAGTTGAGACTTCTGAATATATTTCAAATTTACTTAAAAAACGTGGTATC
    CGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCT
    GGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAA
    TTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAACGACATGAA
    TCTCGTCGTATTGATGACCAGTTACGTGGTCGTTCTGGACGTCAAGGTGATAAAGGGGAT
    AGTCGCTTCTATTTATCATTACAAGATGAATTAATGATTCGTTTTGGTTCTGAACGTTTA
    CAGAAAATGATGAGCCGACTAGGTTTAGATGACTCTACACCAATTGAATCAAAAATGGTA
    TCAAGAGCTGTAGAATCAGCACAAAAACGTGTAGAAGGTAATAACTTCGACGCGCGTAAA
    CGTATCTTAGAATACGATGAAGTATTACGTAAACAACGTGAAATTATCTATAACGAAAGA
    AATAGTATTATTGATGAAGAAGACAGCTCTCAAGTTGTAGATGCAATGCTACGTTCAACG
    TTACAACGTAGTATCAATTACTATATTAATACAGCAGATGACGAGCCTGAATATCAACCA
    TTCATCGACTACATTAATGACATTTTCTTACAAGAAGGTGACATTACAGAGGATGATATC
    AAAGGTAAAGATGCTGAAGATATTTTCGAAGTCGTTTGGGCTAAGATTGAAGCAGCATAT
    CAAAGTCAAAAAGATATCTTAGAAGAACAAATGAATGAGTTTGAGCGTATGATTTTACTT
    CGTTCTATTGATAGCCATTGGACTGATCATATCGACACAATGGATCAATTACGTCAAGGT
    ATTCACTTACGTTCTTATGCACAGCAAAATCCATTACGTGACTATCAAAATGAAGGTCAT
    GAATTATTTGATATCATGATGCAAAATATCGAAGAAGATACTTGTAAATTCATTTTAAAA
    TCTGTAGTACAAGTTGAAGATAATATTGAACGTGAAAAAACAACAGAGTTTGGTGAAGCG
    AAGCACGTTTCAGCTGAAGATGGTAAAGAAAAAGTGAAACCGAAACCAATCGTTAAAGGC
    GATCAAGTTGGTCGTAACGATGATTGTCCATGTGGTAGTGGTAAAAAATTCAAAAATTGC
    CATGGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2532

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0708 [new locus tag: SA_RS04025 ]
  • symbol: SecA
  • description: preprotein translocase subunit SecA
  • length: 843
  • theoretical pI: 4.86612
  • theoretical MW: 95959.5
  • GRAVY: -0.62242

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking preprotein translocase, SecA subunit (TIGR00963; HMM-score: 1129)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system translocase SecA2 (TIGR04397; HMM-score: 933.2)
    and 3 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system translocase SecA2 (TIGR03714; HMM-score: 852)
    accessory Sec system translocase SecA2 (TIGR04221; HMM-score: 615.2)
    SWIM/SEC-C metal-binding motif protein, PBPRA1643 family (TIGR04102; HMM-score: 25)
  • TheSEED  :
    • Protein translocase subunit SecA
    CBSS-393121.3.peg.2760  Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)
    and 1 more
    tRNA synthetase relationships  Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)
  • PFAM:
    P-loop_NTPase (CL0023) SecA_DEAD; SecA DEAD-like domain (PF07517; HMM-score: 397.2)
    and 5 more
    no clan defined SecA_SW; SecA Wing and Scaffold domain (PF07516; HMM-score: 193.4)
    SecA_PP_bind; SecA preprotein cross-linking domain (PF01043; HMM-score: 140.4)
    SEC-C; SEC-C motif (PF02810; HMM-score: 35.2)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 20.3)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 15.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001789
    • TAT(Tat/SPI): 0.000231
    • LIPO(Sec/SPII): 0.000414
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKTKQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGDIAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGLNLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSILIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAERMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRRFSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYNMTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEYISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEELGGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSRLGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSIIDEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKGKDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQGIHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFGEAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA1029(ftsZ)cell division protein FtsZ  [2] (data from MRSA252)
    SA0505(fus)elongation factor G  [2] (data from MRSA252)
    SA1305(hu)DNA-binding protein II  [2] (data from MRSA252)
    SA0448(metS)methionyl-tRNA synthetase  [2] (data from MRSA252)
    SA0943-1(pdhA)pyruvate dehydrogenase E1 component subunit alpha  [2] (data from MRSA252)
    SA0945(pdhC)branched-chain alpha-keto acid dehydrogenase E2 subunit  [2] (data from MRSA252)
    SA0946(pdhD)dihydrolipoamide dehydrogenase  [2] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [2] (data from MRSA252)
    SA2046(rplD)50S ribosomal protein L4  [2] (data from MRSA252)
    SA1084(rplS)50S ribosomal protein L19  [2] (data from MRSA252)
    SA1099(rpsB)30S ribosomal protein S2  [2] (data from MRSA252)
    SA2038(rpsQ)30S ribosomal protein S17  [2] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [2] (data from MRSA252)
    SA0802hypothetical protein  [2] (data from MRSA252)
    SA0829hypothetical protein  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA0708&oldid=90161"