PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0394 [new locus tag: SA_RS02260 ]
• pan locus tag^?: SAUPAN002130000
• symbol: SA0394
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0394 [new locus tag: SA_RS02260 ]
• symbol: SA0394
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 454848..456335
• length: 1488
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123178 NCBI
• RefSeq: NP_373645 NCBI
• BioCyc: see SA_RS02260
• MicrobesOnline: 102671 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAGAGAAAATTTTAAATTACGTAAAATGAAAGTCGGTTTAGTATCAGTTGCAATTACT
  ATGTTATATATCATGACAAACGGACAAGCTGAAGCATCTGAAACTAATCAGAAGGTAAGT
  ACAAATCAAGAATCAAAAGCTGTTTCGCAGACTGAACAAAATTCCAAAGAAACAAAAGCG
  GCAGAGTCTTCTAAAAACTTTGTAAATTTAGATCCTATCAAACCAGGAGCTCAAAAAGTA
  ACAGGTACTACATTACCGAATCATATTATTTTACTTAATATAGATGGTAAAAGTGCTGAT
  TCAGTTGAAGGAGGATATGGTGATTTTATAACTGCAAATGACAAAGGAGAGTTTGAGTAT
  CCTTTAAATAATCGTAAAATTGTCCATAACCAAGAAATTGAGGTATCATCATCAAGCCCT
  GATTTAGGTGACGATGAAGAGGATGAAGAAGTAGAAGAATCTTCAACTGAAAAAGCTGGT
  GCTGAGGAAGAAAGTACAGATGCTAAAGCTACATATACAACACCACGATATGAAAAAGCG
  TATGAAATACCGAAAGAACAACTAAAAGAAAAAGATGGACACCACCAAGTTTTCATCGAA
  CCAATTACTGAAGGTTCAGGTATTATTAAAGGTCATACGTCTGTAAAAGGTAAAGTTGCT
  CTATCTATTAATAATAAATTTATTAATTTTGAAGAAAGAGCTAAAGGTGGAATTAGTAAA
  GAAGATACTAAAGCTAGTTCCGATGGTGTTTGGATGCCTATTAATGAAAAAGGATATTTT
  GATTTTGATTTCAAAAAAAATCCTTTTGATAACTTAGAGTTAAAGAAAAATGATGAAATC
  TCATTAACATTTGCACCTGATGATGAAGATGAAGCATTGAAGTCATTAATTTTCAAAACT
  AAAGTAACGAGCTTAGAAGATATCGATAAAGCAGAAACAAAATATGACCATACTAAAGTG
  GAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATATACATGTAGATGAAATTTACGGA
  AGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAACAAGGAACTAAAGAGATT
  ACAGGGAAGACTAAATTCGCGAATGCAGTAGTGAAAGTATATTCTGACTTAGGTGATGCG
  CAATTGTTCCCTGATATTCAAGTAGATGAAAATGGTAAATTTAGCTTTGATGCTGAAAAA
  GCTGGTTTCAGATTGCAAAATGGAGAAACATTGAATTTTGCAGTAGTTAAACCTATTACT
  GGTGACCTATTACATCAAGGATTTGTCTCTAAATATATTGATGTATATGAATCTCCGGAA
  GAAAAGAAAGAACGTGAATTTGAAGAAAAACTTGAAAACACGCCTGCATATCATAAATTA
  CATGGTGATAAAATTGTAGGATATGATGTTCAAGGTAATCCATCAACTTGGTTCTATCCA
  TTAGGTGAAAAGAAAGTTGAACGTACAACACCTAAATTAGAAAAATAA
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⊟Protein

⊟General

• locus tag: SA0394 [new locus tag: SA_RS02260 ]
• symbol: SA0394
• description: hypothetical protein
• length: 495
• theoretical pI: 4.81306
• theoretical MW: 55498.4
• GRAVY: -0.795152

⊟Function

• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 18.1)
• TheSEED  :
  • Hypothetical protein within Pathogenicity island SaPIn2
• PFAM:
  no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 15.5)
  FimbA (CL0450) Mfa_like_2; Fimbrillin-like (PF15415; HMM-score: 15.4)
  and 1 more
  no clan defined FAM176; FAM176 family (PF14851; HMM-score: 5.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• LocateP: N-terminally anchored (with CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 3
  • Predicted Cleavage Site: GQAEASET
• SignalP: Signal peptide SP(Sec/SPI) length 32 aa
  • SP(Sec/SPI): 0.990948
  • TAT(Tat/SPI): 0.003166
  • LIPO(Sec/SPII): 0.002546
  • Cleavage Site: CS pos: 32-33. AEA-SE. Pr: 0.9448
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926112 NCBI
• RefSeq: NP_373645 NCBI
• UniProt: A0A0H3JNR9 UniProt

⊟Protein sequence

• MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASETNQKVSTNQESKAVSQTEQNSKETKAAESSKNFVNLDPIKPGAQKVTGTTLPNHIILLNIDGKSADSVEGGYGDFITANDKGEFEYPLNNRKIVHNQEIEVSSSSPDLGDDEEDEEVEESSTEKAGAEEESTDAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHTSVKGKVALSINNKFINFEERAKGGISKEDTKASSDGVWMPINEKGYFDFDFKKNPFDNLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLKDVKEDIHVDEIYGSLYHTEKGKGILDKQGTKEITGKTKFANAVVKVYSDLGDAQLFPDIQVDENGKFSFDAEKAGFRLQNGETLNFAVVKPITGDLLHQGFVSKYIDVYESPEEKKEREFEEKLENTPAYHKLHGDKIVGYDVQGNPSTWFYPLGEKKVERTTPKLEK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: set15 > SA0394

⊟Regulation

• regulator: SaeR (activation) regulon

  SaeR

  (TF)

  important in Virulence; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications