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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA0394 [new locus tag: SA_RS02260 ]
- pan locus tag?: SAUPAN002130000
- symbol: SA0394
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Additional information (user-provided)[edit | edit source]
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA0394 [new locus tag: SA_RS02260 ]
- symbol: SA0394
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 454848..456335
- length: 1488
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1123178 NCBI
- RefSeq: NP_373645 NCBI
- BioCyc: see SA_RS02260
- MicrobesOnline: 102671 MicrobesOnline
⊟Phenotype[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟DNA sequence[edit | edit source]
- 1
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1441ATGAGAGAAAATTTTAAATTACGTAAAATGAAAGTCGGTTTAGTATCAGTTGCAATTACT
ATGTTATATATCATGACAAACGGACAAGCTGAAGCATCTGAAACTAATCAGAAGGTAAGT
ACAAATCAAGAATCAAAAGCTGTTTCGCAGACTGAACAAAATTCCAAAGAAACAAAAGCG
GCAGAGTCTTCTAAAAACTTTGTAAATTTAGATCCTATCAAACCAGGAGCTCAAAAAGTA
ACAGGTACTACATTACCGAATCATATTATTTTACTTAATATAGATGGTAAAAGTGCTGAT
TCAGTTGAAGGAGGATATGGTGATTTTATAACTGCAAATGACAAAGGAGAGTTTGAGTAT
CCTTTAAATAATCGTAAAATTGTCCATAACCAAGAAATTGAGGTATCATCATCAAGCCCT
GATTTAGGTGACGATGAAGAGGATGAAGAAGTAGAAGAATCTTCAACTGAAAAAGCTGGT
GCTGAGGAAGAAAGTACAGATGCTAAAGCTACATATACAACACCACGATATGAAAAAGCG
TATGAAATACCGAAAGAACAACTAAAAGAAAAAGATGGACACCACCAAGTTTTCATCGAA
CCAATTACTGAAGGTTCAGGTATTATTAAAGGTCATACGTCTGTAAAAGGTAAAGTTGCT
CTATCTATTAATAATAAATTTATTAATTTTGAAGAAAGAGCTAAAGGTGGAATTAGTAAA
GAAGATACTAAAGCTAGTTCCGATGGTGTTTGGATGCCTATTAATGAAAAAGGATATTTT
GATTTTGATTTCAAAAAAAATCCTTTTGATAACTTAGAGTTAAAGAAAAATGATGAAATC
TCATTAACATTTGCACCTGATGATGAAGATGAAGCATTGAAGTCATTAATTTTCAAAACT
AAAGTAACGAGCTTAGAAGATATCGATAAAGCAGAAACAAAATATGACCATACTAAAGTG
GAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATATACATGTAGATGAAATTTACGGA
AGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGATAAACAAGGAACTAAAGAGATT
ACAGGGAAGACTAAATTCGCGAATGCAGTAGTGAAAGTATATTCTGACTTAGGTGATGCG
CAATTGTTCCCTGATATTCAAGTAGATGAAAATGGTAAATTTAGCTTTGATGCTGAAAAA
GCTGGTTTCAGATTGCAAAATGGAGAAACATTGAATTTTGCAGTAGTTAAACCTATTACT
GGTGACCTATTACATCAAGGATTTGTCTCTAAATATATTGATGTATATGAATCTCCGGAA
GAAAAGAAAGAACGTGAATTTGAAGAAAAACTTGAAAACACGCCTGCATATCATAAATTA
CATGGTGATAAAATTGTAGGATATGATGTTCAAGGTAATCCATCAACTTGGTTCTATCCA
TTAGGTGAAAAGAAAGTTGAACGTACAACACCTAAATTAGAAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA0394 [new locus tag: SA_RS02260 ]
- symbol: SA0394
- description: hypothetical protein
- length: 495
- theoretical pI: 4.81306
- theoretical MW: 55498.4
- GRAVY: -0.795152
⊟Function[edit | edit source]
- TIGRFAM: gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 18.1)
- TheSEED :
- Hypothetical protein within Pathogenicity island SaPIn2
- PFAM: Glutaminase_I (CL0014) PylD_N; Pyrrolysine biosynthesis protein PylD, N-terminal domain (PF21455; HMM-score: 11.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 3.33
- Cellwall Score: 3.33
- Extracellular Score: 3.33
- Internal Helices: 0
- DeepLocPro: Cell wall & surface
- Cytoplasmic Score: 0.0039
- Cytoplasmic Membrane Score: 0.0137
- Cell wall & surface Score: 0.5844
- Extracellular Score: 0.398
- LocateP: N-terminally anchored (with CS)
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: -0.17
- Signal peptide possibility: 1
- N-terminally Anchored Score: 3
- Predicted Cleavage Site: GQAEASET
- SignalP: Signal peptide SP(Sec/SPI) length 32 aa
- SP(Sec/SPI): 0.990948
- TAT(Tat/SPI): 0.003166
- LIPO(Sec/SPII): 0.002546
- Cleavage Site: CS pos: 32-33. AEA-SE. Pr: 0.9448
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟Protein sequence[edit | edit source]
- MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASETNQKVSTNQESKAVSQTEQNSKETKAAESSKNFVNLDPIKPGAQKVTGTTLPNHIILLNIDGKSADSVEGGYGDFITANDKGEFEYPLNNRKIVHNQEIEVSSSSPDLGDDEEDEEVEESSTEKAGAEEESTDAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHTSVKGKVALSINNKFINFEERAKGGISKEDTKASSDGVWMPINEKGYFDFDFKKNPFDNLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLKDVKEDIHVDEIYGSLYHTEKGKGILDKQGTKEITGKTKFANAVVKVYSDLGDAQLFPDIQVDENGKFSFDAEKAGFRLQNGETLNFAVVKPITGDLLHQGFVSKYIDVYESPEEKKEREFEEKLENTPAYHKLHGDKIVGYDVQGNPSTWFYPLGEKKVERTTPKLEK
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: SaeR (activation) regulon
SaeR (TF) important in Virulence; RegPrecise
⊟Additional information (user-provided)[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟Other information (user-provided)[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]