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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000369
  • pan locus tag?: SAUPAN002130000
  • symbol: JSNZ_000369
  • pan gene symbol?: —
  • synonym:
  • product: FKLRK protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000369
  • symbol: JSNZ_000369
  • product: FKLRK protein
  • replicon: chromosome
  • strand: +
  • coordinates: 402326..403828
  • length: 1503
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGGGAAAATTTTAAATTACGTAAAGTGAAAGTCGGTTTAGTATCTGTTGCAATTACT
    ATGTTATATATCATGTCAAACGGACAAGCAGAAGCATCTGAAACTAAAGTAACAAATACA
    AATCAAGGTATAAATACTGTTTCAGAAACTGTACATAATTCAAAAGAATCAAAAAATGAA
    GAAGCTAATAAGAACTTTGTAAAATTAGATACAATTAATCCGGGAGATCAAAAGGTGACA
    GGTACTACTTTACCGCATCACTTAATATTATTGAATGTCGATAAACGTAGTGTAGATTCA
    GTAGAAGATAGTGGACTCGGAATTGTTACGGCTGGAGAAGATGGTAGATTTGAATATAAC
    TTAAAAGATCATAAAATTGTTCATAATCAAGAGATAGAAGTAACATCGTCTAGTCCTGAT
    TTAGGAGAAGATGATGAAGAAGTTGAAGAGAAAGCTTCTAATCAAAAAGAGGAAACAGCA
    AATAAAAAAGATGAAGACGAATCTAATGATGTCAAAACTACTGTCACAACACCAAGGTAT
    GAAAAAGCATATAAAGTTCCAAATCAACAATTAAAACAAGAAAATGGACATCATCAAGTA
    TTAGTTGAACCAATTACCGCAGGATCTGGTATCATTAAAGGTCATACTTCGGTAAAAGGA
    AAAGTTGCTCTTTCAATTAATAATAAATTTATTAATCTTGGTGATAGTACTGATGTTGAA
    AATCGTTTAACTGAAGACCAAGGAAAAACTAGGTATCAAGGTATTTGGAAACATATAGAT
    GATAAAGGTTATTTTGAATTTGATTTCAAACGTAAACCATATGATAACTTAAAATTGAAT
    CCAGATGATAATGTGTCATTAACATTCTTGCCAAACGATGAAGAAGAGGCTTTAAAGCCA
    ATTATATTTAAAACAAAAGTAACAAGTTTTGATAATATTGAATCTGCTATAACTAGCTAT
    AATCCTAAAAATATCAATAAAGTAACAACTCTTACTAATGTAAAAGAAGACTTAGTAGTT
    GAAGATATTTATGGCTTTGAATATAAAAGTGAACAAGGAAGAGGAATTCCTCAAAATAAT
    AGCCATAGTACTAAAGAAATTAAAGGTAAAACAAAATTTGCGAATGCAGTTGTAAAAGTT
    GATTCTGAATTAGGTGAAGGTCAAGAATTCCCAGATTTACAAGTTAATGAAAAAGGTGAA
    TTCACATTCAATTCATATGATGCTGGTTATAGATTATACAATGGAGAAACTTTAAACTTT
    GTTATTGTAGATCCTATTACTGGTGTTCAGTTGAGTCAAGGATTTGTGAGCAAATATATA
    GATGTTTATGAATCGCCTGAGCAAAAGAAAGAGCGCGAATTTGATGAAAAACTTGAAAGT
    ACTCCAGCTTATTACCAATTACATGGGGATAAAATAATAGGTTTTGATACTGAGGGGGGT
    GTATCAACTTGGTTTGATGCACTTGATAAGAAAGAACTGGAAAAGGTTCTTCGTCAATAT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1503

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000369
  • symbol: JSNZ_000369
  • description: FKLRK protein
  • length: 500
  • theoretical pI: 4.77472
  • theoretical MW: 56407.1
  • GRAVY: -0.7912

⊟Function[edit | edit source]

  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 19.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    E-set (CL0159) Big_6; Bacterial Ig domain (PF17936; HMM-score: 22.1)
    and 2 more
    no clan defined Fve; Fungal immunomodulatory protein Fve (PF09259; HMM-score: 16)
    Glutaminase_I (CL0014) PylD_N; Pyrrolysine biosynthesis protein PylD, N-terminal domain (PF21455; HMM-score: 15.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0017
    • Cytoplasmic Membrane Score: 0.005
    • Cell wall & surface Score: 0.2734
    • Extracellular Score: 0.7199
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 32 aa
    • SP(Sec/SPI): 0.993841
    • TAT(Tat/SPI): 0.002161
    • LIPO(Sec/SPII): 0.001771
    • Cleavage Site: CS pos: 32-33. AEA-SE. Pr: 0.9572
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MRENFKLRKVKVGLVSVAITMLYIMSNGQAEASETKVTNTNQGINTVSETVHNSKESKNEEANKNFVKLDTINPGDQKVTGTTLPHHLILLNVDKRSVDSVEDSGLGIVTAGEDGRFEYNLKDHKIVHNQEIEVTSSSPDLGEDDEEVEEKASNQKEETANKKDEDESNDVKTTVTTPRYEKAYKVPNQQLKQENGHHQVLVEPITAGSGIIKGHTSVKGKVALSINNKFINLGDSTDVENRLTEDQGKTRYQGIWKHIDDKGYFEFDFKRKPYDNLKLNPDDNVSLTFLPNDEEEALKPIIFKTKVTSFDNIESAITSYNPKNINKVTTLTNVKEDLVVEDIYGFEYKSEQGRGIPQNNSHSTKEIKGKTKFANAVVKVDSELGEGQEFPDLQVNEKGEFTFNSYDAGYRLYNGETLNFVIVDPITGVQLSQGFVSKYIDVYESPEQKKEREFDEKLESTPAYYQLHGDKIIGFDTEGGVSTWFDALDKKELEKVLRQY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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