PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000369
• pan locus tag^?: SAUPAN002130000
• symbol: JSNZ_000369
• pan gene symbol^?: —
• synonym:
• product: FKLRK protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000369
• symbol: JSNZ_000369
• product: FKLRK protein
• replicon: chromosome
• strand: +
• coordinates: 402326..403828
• length: 1503
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  
  ATGAGGGAAAATTTTAAATTACGTAAAGTGAAAGTCGGTTTAGTATCTGTTGCAATTACT
  ATGTTATATATCATGTCAAACGGACAAGCAGAAGCATCTGAAACTAAAGTAACAAATACA
  AATCAAGGTATAAATACTGTTTCAGAAACTGTACATAATTCAAAAGAATCAAAAAATGAA
  GAAGCTAATAAGAACTTTGTAAAATTAGATACAATTAATCCGGGAGATCAAAAGGTGACA
  GGTACTACTTTACCGCATCACTTAATATTATTGAATGTCGATAAACGTAGTGTAGATTCA
  GTAGAAGATAGTGGACTCGGAATTGTTACGGCTGGAGAAGATGGTAGATTTGAATATAAC
  TTAAAAGATCATAAAATTGTTCATAATCAAGAGATAGAAGTAACATCGTCTAGTCCTGAT
  TTAGGAGAAGATGATGAAGAAGTTGAAGAGAAAGCTTCTAATCAAAAAGAGGAAACAGCA
  AATAAAAAAGATGAAGACGAATCTAATGATGTCAAAACTACTGTCACAACACCAAGGTAT
  GAAAAAGCATATAAAGTTCCAAATCAACAATTAAAACAAGAAAATGGACATCATCAAGTA
  TTAGTTGAACCAATTACCGCAGGATCTGGTATCATTAAAGGTCATACTTCGGTAAAAGGA
  AAAGTTGCTCTTTCAATTAATAATAAATTTATTAATCTTGGTGATAGTACTGATGTTGAA
  AATCGTTTAACTGAAGACCAAGGAAAAACTAGGTATCAAGGTATTTGGAAACATATAGAT
  GATAAAGGTTATTTTGAATTTGATTTCAAACGTAAACCATATGATAACTTAAAATTGAAT
  CCAGATGATAATGTGTCATTAACATTCTTGCCAAACGATGAAGAAGAGGCTTTAAAGCCA
  ATTATATTTAAAACAAAAGTAACAAGTTTTGATAATATTGAATCTGCTATAACTAGCTAT
  AATCCTAAAAATATCAATAAAGTAACAACTCTTACTAATGTAAAAGAAGACTTAGTAGTT
  GAAGATATTTATGGCTTTGAATATAAAAGTGAACAAGGAAGAGGAATTCCTCAAAATAAT
  AGCCATAGTACTAAAGAAATTAAAGGTAAAACAAAATTTGCGAATGCAGTTGTAAAAGTT
  GATTCTGAATTAGGTGAAGGTCAAGAATTCCCAGATTTACAAGTTAATGAAAAAGGTGAA
  TTCACATTCAATTCATATGATGCTGGTTATAGATTATACAATGGAGAAACTTTAAACTTT
  GTTATTGTAGATCCTATTACTGGTGTTCAGTTGAGTCAAGGATTTGTGAGCAAATATATA
  GATGTTTATGAATCGCCTGAGCAAAAGAAAGAGCGCGAATTTGATGAAAAACTTGAAAGT
  ACTCCAGCTTATTACCAATTACATGGGGATAAAATAATAGGTTTTGATACTGAGGGGGGT
  GTATCAACTTGGTTTGATGCACTTGATAAGAAAGAACTGGAAAAGGTTCTTCGTCAATAT
  TAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1503
  

⊟Protein

⊟General

• locus tag: JSNZ_000369
• symbol: JSNZ_000369
• description: FKLRK protein
• length: 500
• theoretical pI: 4.77472
• theoretical MW: 56407.1
• GRAVY: -0.7912

⊟Function

• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 19.7)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  E-set (CL0159) Big_6; Bacterial Ig domain (PF17936; HMM-score: 22.1)
  and 2 more
  no clan defined Fve; Fungal immunomodulatory protein Fve (PF09259; HMM-score: 16)
  Glutaminase_I (CL0014) PylD_N; Pyrrolysine biosynthesis protein PylD, N-terminal domain (PF21455; HMM-score: 15.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0.0017
  • Cytoplasmic Membrane Score: 0.005
  • Cell wall & surface Score: 0.2734
  • Extracellular Score: 0.7199
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 32 aa
  • SP(Sec/SPI): 0.993841
  • TAT(Tat/SPI): 0.002161
  • LIPO(Sec/SPII): 0.001771
  • Cleavage Site: CS pos: 32-33. AEA-SE. Pr: 0.9572
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MRENFKLRKVKVGLVSVAITMLYIMSNGQAEASETKVTNTNQGINTVSETVHNSKESKNEEANKNFVKLDTINPGDQKVTGTTLPHHLILLNVDKRSVDSVEDSGLGIVTAGEDGRFEYNLKDHKIVHNQEIEVTSSSPDLGEDDEEVEEKASNQKEETANKKDEDESNDVKTTVTTPRYEKAYKVPNQQLKQENGHHQVLVEPITAGSGIIKGHTSVKGKVALSINNKFINLGDSTDVENRLTEDQGKTRYQGIWKHIDDKGYFEFDFKRKPYDNLKLNPDDNVSLTFLPNDEEEALKPIIFKTKVTSFDNIESAITSYNPKNINKVTTLTNVKEDLVVEDIYGFEYKSEQGRGIPQNNSHSTKEIKGKTKFANAVVKVDSELGEGQEFPDLQVNEKGEFTFNSYDAGYRLYNGETLNFVIVDPITGVQLSQGFVSKYIDVYESPEQKKEREFDEKLESTPAYYQLHGDKIIGFDTEGGVSTWFDALDKKELEKVLRQY

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR (activation) regulon

  SaeR

  (TF)

  important in Virulence; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications