PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS02895 [old locus tag: NWMN_0496 ]
• pan locus tag^?: SAUPAN002302000
• symbol: NWMN_RS02895
• pan gene symbol^?: sigH
• synonym:
• product: RNA polymerase sigma factor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS02895 [old locus tag: NWMN_0496 ]
• symbol: NWMN_RS02895
• product: RNA polymerase sigma factor
• replicon: chromosome
• strand: +
• coordinates: 570767..571336
• length: 570
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (570767..571336) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0496

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGAAATACGATTTGACAACTCAAGACAGTACAATCAAACGTAACAATGCAATAAATGAT
  AAAGACTTCGAAAAGTTAGTAATGGATCTACAACCATTAATTATTCGACGCATCAAAACA
  TTTGGATTTAATCATTATGATTTAGAAGACTTATATCAAGAAATACTTATACGGATGTAT
  AGGTCGGTCCAAACATTTGATTTTAGTGGAGAGCAGCCTTTCACAAATTATGTTCAATGT
  TTAATTACGTCTGTAAAGTATGATTATTTGAGAAAATATTTAGCTACAAATAAAAGAATG
  GATAATTTGATTAATGAATATAGAGTTACGTATCCATGTGCAATAAAGCGTTTTGATGTT
  GAAAACAATTATTTGAATAAATTAGCAATTAAAGAGTTGATTTGTCAGTTTAAGTATTTG
  AGTGCATTTGAAAAAGATGTCATGTATTTAATGTGTGAACAATATAAGCCGAGAGAAATT
  GCTCAATTGATGCATGTAAAAGAGAAAGTGATTTATAATGCCATACAACGATGTAAAAAT
  AAAATAAAACGTTATTTCAAAATGATTTGA
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⊟Protein

⊟General

• locus tag: NWMN_RS02895 [old locus tag: NWMN_0496 ]
• symbol: NWMN_RS02895
• description: RNA polymerase sigma factor
• length: 189
• theoretical pI: 9.43118
• theoretical MW: 23028.8
• GRAVY: -0.478836

⊟Function

• TIGRFAM:
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 53.2)
  and 17 more
  RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 29)
  Cellular processes Cellular processes Sporulation and germination RNA polymerase sigma-H factor (TIGR02859; HMM-score: 27.8)
  Genetic information processing Transcription Transcription factors RNA polymerase sigma-H factor (TIGR02859; HMM-score: 27.8)
  RNA polymerase sigma-70 factor, sigma-B/F/G subfamily (TIGR02980; HMM-score: 25.2)
  RNA polymerase sigma-70 factor, TIGR02952 family (TIGR02952; HMM-score: 25.1)
  RNA polymerase sigma-70 factor, Rhodopirellula/Verrucomicrobium family (TIGR02989; HMM-score: 25.1)
  Cellular processes Cellular processes Sporulation and germination RNA polymerase sigma-F factor (TIGR02885; HMM-score: 23.8)
  Genetic information processing Transcription Transcription factors RNA polymerase sigma-F factor (TIGR02885; HMM-score: 23.8)
  RNA polymerase sigma factor RpoE (TIGR02939; HMM-score: 21)
  RNA polymerase sigma factor, FliA/WhiG family (TIGR02479; HMM-score: 19.9)
  RNA polymerase sigma factor, SigM family (TIGR02950; HMM-score: 19.8)
  RNA polymerase sigma-70 factor, Planctomycetaceae-specific subfamily 1 (TIGR02984; HMM-score: 19.4)
  RNA polymerase sigma-W factor (TIGR02948; HMM-score: 16.4)
  Cellular processes Cellular processes Sporulation and germination RNA polymerase sigma-G factor (TIGR02850; HMM-score: 15.4)
  Genetic information processing Transcription Transcription factors RNA polymerase sigma-G factor (TIGR02850; HMM-score: 15.4)
  RNA polymerase sigma-70 factor, sigma-E family (TIGR02983; HMM-score: 15.4)
  RNA polymerase sigma-70 factor, TIGR02954 family (TIGR02954; HMM-score: 12.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  HTH (CL0123) Sigma70_r2; Sigma-70 region 2 (PF04542; HMM-score: 42.7)
  and 7 more
  Terminase_5; Putative ATPase subunit of terminase (gpP-like) (PF06056; HMM-score: 16.1)
  Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 14.6)
  UPF0122; Putative helix-turn-helix protein, YlxM / p13 like (PF04297; HMM-score: 13.7)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 13.4)
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 12.9)
  GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 11.8)
  HTH_29; Winged helix-turn helix (PF13551; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by SigH*, sigma factor controlling competence and phage integrase genes: in N315, NCTC8325

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001521
  • TAT(Tat/SPI): 0.000208
  • LIPO(Sec/SPII): 0.000353
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446795617 NCBI
• RefSeq: WP_000872873 NCBI
• UniProt: see NWMN_0496

⊟Protein sequence

• MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFNHYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDNLINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREIAQLMHVKEKVIYNAIQRCKNKIKRYFKMI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications