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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS02860 [old locus tag: NWMN_0489 ]
  • pan locus tag?: SAUPAN002293000
  • symbol: NWMN_RS02860
  • pan gene symbol?: —
  • synonym:
  • product: PIN/TRAM domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS02860 [old locus tag: NWMN_0489 ]
  • symbol: NWMN_RS02860
  • product: PIN/TRAM domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 563491..564546
  • length: 1056
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (563491..564546) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0489

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGTTATTATTATTTACTTAATTATTGGGAGCGCATTAGGAATAATTATTATTCCTGAA
    ATTGCAAATGATCTTGGATTACAAAACTCCAGCTTTTTAAAAAATCACTATGTAGATGGC
    ATTATCGGTAGTATTTTTATGTTCTTAATTTTTGGTGTATTTATTAGACGAGTTACTAAC
    GCTATAAAAGGTTTAGAACATTTTATTATGCGTAGAAGTGCTGTTGAAATACTATTCGCA
    ACAATAGGTTTAATAATCGGATTACTTATTTCTGTTATGGTGTCGTTTATATTAGAATCA
    ATTGGCAACTCTATTTTTAATCATTTCATTCCTGTCATAATTACGATATTACTATGTTAT
    TTCGGTTTCCAATTTGGCCTTAAAAAACGAGATGAAATGTTAATGTTTTTACCTGAGAAT
    ATAGCGCGTTCCATGTCACAACATACTAAAAGTGCTACGCCAAAAATTATCGACACAAGC
    GCAATTATTGATGGTCGTATTTTAGAAGTCATTCGTTGCGGTTTTATCGATGGCAATATT
    TTAATTCCACAAGGTGTTATTAATGAATTACAAATTGTTGCAGATTCAAATGACAGTGTT
    AAACGTGAAAAGGGTAAAAGAGGCTTAGATATTTTAAATGAATTGTATGATTTAGACTAT
    CCTACAAAGGTTATACATCCAACTAAAACACATAGTGATATTGATACGATGTTATTAAAA
    TTAGCAAAACAATATCATGCAAGTATTATAACGACAGATTTCAACCTAAATAAAGTTTGT
    CATGTACATGGTATCAAAGCATTAAATGTTAATGATTTATCAGAAGCAATCAAACCTAAT
    GTACATCAAGGTGATCAACTGCATATTTTACTGACAAAAATGGGTAAGGAGCCTGGTCAG
    GCAGTAGGATATCTAGATGATGGTACGATGGTAGTTGTTGATAATGCTAAAAATCTTATT
    GGCAGTCATGTCAATTTAGAAGTAGTCAGCTTATTGCAAACATCTTCAGGAAGAATTGTT
    TTTGCTAAAAAAATCGAAGATACAGTATCATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1056

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS02860 [old locus tag: NWMN_0489 ]
  • symbol: NWMN_RS02860
  • description: PIN/TRAM domain-containing protein
  • length: 351
  • theoretical pI: 7.15961
  • theoretical MW: 38887.5
  • GRAVY: 0.400285

⊟Function[edit | edit source]

  • TIGRFAM:
    RNA ligase partner, MJ_0950 family (TIGR03875; HMM-score: 15.5)
    Metabolism Energy metabolism Other tetrahydromethanopterin S-methyltransferase, subunit C (TIGR01148; EC 2.1.1.86; HMM-score: 12.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    OB (CL0021) TRAM; TRAM domain (PF01938; HMM-score: 17.6)
    PIN (CL0280) PIN; PIN domain (PF01850; HMM-score: 16.5)
    PIN_5; PINc domain ribonuclease (PF08745; HMM-score: 14.8)
    and 5 more
    no clan defined MtrC; Tetrahydromethanopterin S-methyltransferase, subunit C (PF04211; HMM-score: 14)
    LysE (CL0292) DsbD_2; Cytochrome C biogenesis protein transmembrane region (PF13386; HMM-score: 13.4)
    Colicin_V; Colicin V production protein (PF02674; HMM-score: 10.3)
    no clan defined Virul_fac_BrkB; Virulence factor BrkB (PF03631; HMM-score: 7.2)
    DUF4514; Domain of unknown function (DUF4514) (PF14986; HMM-score: 6.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.07
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.18
    • Extracellular Score: 0.21
    • Internal Helices: 4
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.020825
    • TAT(Tat/SPI): 0.000169
    • LIPO(Sec/SPII): 0.008156
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYVDGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSFILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATPKIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDILNELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNVNDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEVVSLLQTSSGRIVFAKKIEDTVSL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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