From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0489 [new locus tag: NWMN_RS02860 ]
  • pan locus tag?: SAUPAN002293000
  • symbol: NWMN_0489
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0489 [new locus tag: NWMN_RS02860 ]
  • symbol: NWMN_0489
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 563473..564546
  • length: 1074
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    GTGAATATCGTTAAACTAATGGTTATTATTATTTACTTAATTATTGGGAGCGCATTAGGA
    ATAATTATTATTCCTGAAATTGCAAATGATCTTGGATTACAAAACTCCAGCTTTTTAAAA
    AATCACTATGTAGATGGCATTATCGGTAGTATTTTTATGTTCTTAATTTTTGGTGTATTT
    ATTAGACGAGTTACTAACGCTATAAAAGGTTTAGAACATTTTATTATGCGTAGAAGTGCT
    GTTGAAATACTATTCGCAACAATAGGTTTAATAATCGGATTACTTATTTCTGTTATGGTG
    TCGTTTATATTAGAATCAATTGGCAACTCTATTTTTAATCATTTCATTCCTGTCATAATT
    ACGATATTACTATGTTATTTCGGTTTCCAATTTGGCCTTAAAAAACGAGATGAAATGTTA
    ATGTTTTTACCTGAGAATATAGCGCGTTCCATGTCACAACATACTAAAAGTGCTACGCCA
    AAAATTATCGACACAAGCGCAATTATTGATGGTCGTATTTTAGAAGTCATTCGTTGCGGT
    TTTATCGATGGCAATATTTTAATTCCACAAGGTGTTATTAATGAATTACAAATTGTTGCA
    GATTCAAATGACAGTGTTAAACGTGAAAAGGGTAAAAGAGGCTTAGATATTTTAAATGAA
    TTGTATGATTTAGACTATCCTACAAAGGTTATACATCCAACTAAAACACATAGTGATATT
    GATACGATGTTATTAAAATTAGCAAAACAATATCATGCAAGTATTATAACGACAGATTTC
    AACCTAAATAAAGTTTGTCATGTACATGGTATCAAAGCATTAAATGTTAATGATTTATCA
    GAAGCAATCAAACCTAATGTACATCAAGGTGATCAACTGCATATTTTACTGACAAAAATG
    GGTAAGGAGCCTGGTCAGGCAGTAGGATATCTAGATGATGGTACGATGGTAGTTGTTGAT
    AATGCTAAAAATCTTATTGGCAGTCATGTCAATTTAGAAGTAGTCAGCTTATTGCAAACA
    TCTTCAGGAAGAATTGTTTTTGCTAAAAAAATCGAAGATACAGTATCATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1074

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0489 [new locus tag: NWMN_RS02860 ]
  • symbol: NWMN_0489
  • description: hypothetical protein
  • length: 357
  • theoretical pI: 7.4154
  • theoretical MW: 39586.4
  • GRAVY: 0.413165

Function[edit | edit source]

  • TIGRFAM:
    RNA ligase partner, MJ_0950 family (TIGR03875; HMM-score: 15.4)
    and 1 more
    Metabolism Energy metabolism Other tetrahydromethanopterin S-methyltransferase, subunit C (TIGR01148; EC 2.1.1.86; HMM-score: 11.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    OB (CL0021) TRAM; TRAM domain (PF01938; HMM-score: 17.6)
    PIN (CL0280) PIN; PIN domain (PF01850; HMM-score: 16.5)
    PIN_5; PINc domain ribonuclease (PF08745; HMM-score: 14.8)
    and 5 more
    LysE (CL0292) DsbD_2; Cytochrome C biogenesis protein transmembrane region (PF13386; HMM-score: 13.4)
    no clan defined MtrC; Tetrahydromethanopterin S-methyltransferase, subunit C (PF04211; HMM-score: 12)
    LysE (CL0292) Colicin_V; Colicin V production protein (PF02674; HMM-score: 10)
    no clan defined Virul_fac_BrkB; Virulence factor BrkB (PF03631; HMM-score: 6.6)
    DUF4514; Domain of unknown function (DUF4514) (PF14986; HMM-score: 6.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.07
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.18
    • Extracellular Score: 0.21
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.073056
    • TAT(Tat/SPI): 0.000271
    • LIPO(Sec/SPII): 0.041682
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYVDGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSFILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATPKIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDILNELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNVNDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEVVSLLQTSSGRIVFAKKIEDTVSL

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]