PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0485 [new locus tag: SA_RS02855 ]
• pan locus tag^?: SAUPAN002293000
• symbol: SA0485
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0485 [new locus tag: SA_RS02855 ]
• symbol: SA0485
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 567016..568089
• length: 1074
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123289 NCBI
• RefSeq: NP_373737 NCBI
• BioCyc: see SA_RS02855
• MicrobesOnline: 102763 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
  
  GTGAATATCGTTAAACTAATGGTTATTATTATTTACTTAATTATTGGGAGCGCATTAGGA
  ATAATTATTATTCCTGAAATTGCAAATGATCTTGGATTACAAAACTCCAGCTTTTTAAAA
  AATCACTATGTAGATGGCATTATCGGTAGTATTTTTATGTTCTTAATTTTTGGTGTATTT
  ATTAGACGAGTTACTAACGCTATAAAAGGTTTAGAACATTTTATTATGCGTAGAAGTGCT
  GTTGAAATACTATTCGCAACAATAGGTTTAATAATCGGATTACTTATTTCTGTTATGGTG
  TCGTTTATATTAGAATCAATTGGTAACTCTATTTTTAATCATTTCATTCCTGTCATAATT
  ACGATATTACTATGTTATTTCGGTTTCCAATTTGGCCTTAAAAAACGAGATGAAATGTTA
  ATGTTTTTACCTGAGAATATAGCGCGTTCCATGTCACAACATACTAAAAGTGCTACGCCA
  AAAATTATCGACACAAGCGCAATTATTGATGGTCGTATTTTAGAAGTCATTCGTTGCGGT
  TTTATCGATGGCAATATTTTAATTCCGCAAGGTGTTATTAATGAATTACAAATTGTTGCA
  GATTCAAATGACAGTGTTAAACGTGAAAAGGGTAAAAGAGGCTTAGATATTTTAAATGAA
  TTGTATGATTTAGATTATCCTACAAAGGTTATACATCCAACTAAAACACATAGTGATATT
  GATACAATGCTATTAAAGCTAGCTAAACAATATCATGCAAGTATTATTACGACAGATTTC
  AACCTAAATAAAGTTTGTCATGTACATGGTATCAAAGCATTAAATGTTAATGATTTATCA
  GAAGCAATCAAACCTAATGTACATCAAGGTGATCAACTGCATATTTTACTGACAAAAATG
  GGTAAAGAGCCTGGTCAGGCAGTAGGATATCTAGATGATGGTACGATGGTAGTTGTTGAT
  AATGCTAAAAATCTTATTGGCAGTCATGTCAATTTAGAAGTAGTCAGCTTATTGCAAACA
  TCTTCAGGAAGAATTGTTTTTGCTAAAAAAATCGAAGATACAGTATCATTATAA
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  1074
  

⊟Protein

⊟General

• locus tag: SA0485 [new locus tag: SA_RS02855 ]
• symbol: SA0485
• description: hypothetical protein
• length: 357
• theoretical pI: 7.4154
• theoretical MW: 39586.4
• GRAVY: 0.413165

⊟Function

• TIGRFAM:
  RNA ligase partner, MJ_0950 family (TIGR03875; HMM-score: 15.4)
  and 1 more
  Metabolism Energy metabolism Other tetrahydromethanopterin S-methyltransferase, subunit C (TIGR01148; EC 2.1.1.86; HMM-score: 11.2)
• TheSEED  :
  • Membrane-associated protein containing RNA-binding TRAM domain and ribonuclease PIN-domain, YacL B.subtilis ortholog
  Stress Response Heat shock Heat shock dnaK gene cluster extended  Membrane-associated protein containing RNA-binding TRAM domain and ribonuclease PIN-domain, YacL B.subtilis ortholog
• PFAM:
  OB (CL0021) TRAM; TRAM domain (PF01938; HMM-score: 17.6)
  PIN (CL0280) PIN; PIN domain (PF01850; HMM-score: 16.5)
  PIN_5; PINc domain ribonuclease (PF08745; HMM-score: 14.8)
  and 5 more
  LysE (CL0292) DsbD_2; Cytochrome C biogenesis protein transmembrane region (PF13386; HMM-score: 13.4)
  no clan defined MtrC; Tetrahydromethanopterin S-methyltransferase, subunit C (PF04211; HMM-score: 12)
  LysE (CL0292) Colicin_V; Colicin V production protein (PF02674; HMM-score: 10)
  no clan defined Virul_fac_BrkB; Virulence factor BrkB (PF03631; HMM-score: 6.6)
  DUF4514; Domain of unknown function (DUF4514) (PF14986; HMM-score: 6.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.07
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.18
  • Extracellular Score: 0.21
  • Internal Helices: 4
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.073056
  • TAT(Tat/SPI): 0.000271
  • LIPO(Sec/SPII): 0.041682
• predicted transmembrane helices (TMHMM): 4

⊟Accession numbers

• GI: 15926204 NCBI
• RefSeq: NP_373737 NCBI
• UniProt: A0A0H3JKY4 UniProt

⊟Protein sequence

• MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYVDGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSFILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATPKIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDILNELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNVNDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEVVSLLQTSSGRIVFAKKIEDTVSL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: radA > SA0485

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications