PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS01135 [old locus tag: NWMN_0202 ]
• pan locus tag^?: SAUPAN001154000
• symbol: NWMN_RS01135
• pan gene symbol^?: rbsK
• synonym:
• product: ribokinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS01135 [old locus tag: NWMN_0202 ]
• symbol: NWMN_RS01135
• product: ribokinase
• replicon: chromosome
• strand: -
• coordinates: 259885..260799
• length: 915
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (259885..260799) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0202

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
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  361
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  481
  541
  601
  661
  721
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  901
  
  ATGACCAACAAAGTTGTTATTTTAGGTTCAACGAATGTCGATCAATTTTTAACAGTTGAA
  AGATATGCACAACCAGGCGAAACATTACATGTTGAAGAAGCACAAAAAGCATTCGGCGGA
  GGTAAAGGTGCCAACCAGGCTATTGCCACTGCACGCATGCAAGCAGACACAACATTTATT
  ACTAAAATTGGCACTGATGGCGTTGCTGATTTCATCTTAGAAGATTTTAAAGTAGCTCAT
  ATTGATACATCATATATTATCAAAACAGCTGAAGCAAAAACGGGCCAAGCCTTTATCACT
  GTGAATGCAGAAGGACAAAACACCATCTATGTTTATGGTGGTGCGAATATGACGATGACA
  CCTGAAGATGTTATTAACGCAAAAGACGCTATAATCAATGCAGACTTTGTCGTTGCACAA
  TTAGAAGTACCCATCCCGGCTATTATATCTGCATTTGAAATTGCCAAGGCACATGGTGTG
  ACGACAGTATTAAATCCTGCACCAGCGAAAGCATTACCTAATGAATTATTATCATTAATC
  GATATTATTGTGCCAAACGAAACAGAAGCCGAATTGTTATCTGGGATTAAAGTAACTAAT
  GAACAATCTATGAAAGACAATGCCAATTACTTTTTATCTATAGGCATTAAGACTGTTTTG
  ATTACGCTAGGTAAGCAAGGTACATATTTTGCTACTAAAAATCAAAGCCAACACATCGAA
  GCTTATAAAGTAAATGCGATTGATACAACTGCTGCAGGCGACACATTTATTGGTGCATTT
  GTCAGTCGCTTAAACAAGTCGCAAGATAACTTAGCAGATGCTATTGATTTTGGTAATAAA
  GCGAGCTCACTCACTGTACAAAAACACGGCGCGCAAGCATCTATTCCTCTACTAGAAGAA
  GTAAATCAAGTTTAA
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⊟Protein

• 3RY7
  PDB

Protein Data Bank: 3RY7

⊟General

• locus tag: NWMN_RS01135 [old locus tag: NWMN_0202 ]
• symbol: NWMN_RS01135
• description: ribokinase
• length: 304
• theoretical pI: 4.6011
• theoretical MW: 32448.5
• GRAVY: 0.0286184

⊟Function

• reaction:
  EC 2.7.1.15?  ExPASy
  Ribokinase ATP + D-ribose = ADP + D-ribose 5-phosphate
• TIGRFAM:
  Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 379.2)
  and 7 more
  Metabolism Energy metabolism Sugars 5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 114.7)
  hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 91.2)
  1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 83.3)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 80.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 50.7)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides tagatose-6-phosphate kinase (TIGR01231; EC 2.7.1.144; HMM-score: 25.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 25.1)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 227.8)
  and 3 more
  Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 49.9)
  NADP_Rossmann (CL0063) Glyco_hydro_4; Family 4 glycosyl hydrolase (PF02056; HMM-score: 14.2)
  Ig (CL0011) Adeno_E3_CR1; Adenovirus E3 region protein CR1 (PF02440; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.014499
  • TAT(Tat/SPI): 0.001126
  • LIPO(Sec/SPII): 0.001169
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446104896 NCBI
• RefSeq: WP_000182751 NCBI
• UniProt: see NWMN_0202

⊟Protein sequence

• MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGANQAIATARMQADTTFITKIGTDGVADFILEDFKVAHIDTSYIIKTAEAKTGQAFITVNAEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGVTTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSIGIKTVLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAIDFGNKASSLTVQKHGAQASIPLLEEVNQV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see NWMN_0202

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications