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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2080 [new locus tag: NWMN_RS12050 ]
  • pan locus tag?: SAUPAN005570000
  • symbol: NWMN_2080
  • pan gene symbol?: sfnaB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2080 [new locus tag: NWMN_RS12050 ]
  • symbol: NWMN_2080
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2316697..2318454
  • length: 1758
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGTATATCTTGAATGGGCAAAGGCAGATAGAAATATTCAATATCGTGTAATTAACGCC
    ATTATTAAAGAACGTATTTACCCCGAGCAAACATTTATTTCGCAAAAAGGATCTTTAATT
    GAAATTCAGTATCATATGCATGTGTTGACTATTGAAGTTGTTAGAAAAAGTGCATTAGAA
    CGCTATGAGTTTACAGGTGATATTACTTATTTAAATAAAGGTGAAACGTCATTAATTATA
    ACTTTAGAAGGTTTATTAGATGTGTTGAATCATGACTTTGATATCCCTATTTCAGAGCGA
    CTACGCGAAGAGTTAATACACAGTCGAGATAGTTTAGTTGAAACATATAAGCAAATGTCT
    CACAGACAAACGTTAATAAGTCAAAGTTTTAAATTTTCAAGGTTACCACAAGATATTAAC
    TTTTTTTCATGGTTACAACATGTAAAAGATAGTGATAAGACAGATGATTTAACTTATTCT
    GAGAGTTTGGTACCAGAGGGGCATCCAACACACCCTTTAACCAAAACGAAATTGCCCTTA
    ACTATGGAAGAAGTACGAGCATATGCACCTGAGTTTGAAAAAGAAATCCCTTTGCAAATT
    ATGATGATTGAAAAAGACCATGTTGTGTGCACAGCTATGGATGGTAATGATCAATTTATT
    ATTGATGAAATAATTCCCGAATACTACAATCAGATTCGTGTGTTTTTAAAGAGTTTAGGT
    TTGAAAAGTGAAGACTATAGAGCGATTTTAGTACATCCTTGGCAATATGATCATACGATA
    GGGAAATATTTTGAAGCATGGATTGCTAAAAAAATATTAATTCCAACGCCGTTTACAATA
    CTTTCAAAAGCAACTTTATCATTTAGGACGATGTCTTTAATTGATAAACCATACCATGTT
    AAGTTGCCCGTCGATGCACAAGCAACAAGTGCCGTTAGAACAGTCTCAACTGTGACTACT
    GTAGATGGACCAAAGTTAAGTTATGCTTTACAAAACATGTTGAATCAATATCCAGGATTT
    AAAGTTGCTATGGAACCGTTCGGTGAATATGCAAATGTTGATAAAGATAGGGCACGTCAG
    CTTGCTTGTATTATAAGACAAAAGCCTGAAATTGATGGAAAAGGTGCAACAGTAGTTAGT
    GCAAGTCTAGTTAATAAAAATCCAATAGATCAAAAAGTTATCGTGGATAGTTACTTAGAG
    TGGTTAAATCAAGGAATTACTAAAGAAAGTATTACGACATTTATTGAACGATACGCTCAA
    GCATTAATCCCGCCTTTAATTGCTTTTATTCAAAATTATGGAATTGCTTTAGAAGCACAC
    ATGCAAAATACAGTAGTGAACTTGGGGCCACATTTTGACATTCAATTTTTAGTGAGAGAT
    TTAGGTGGTTCTAGAATTGATTTAGAAACATTACAACATCGTGTATCAGATATTAAAATT
    ACAAATGATAGTTTAATAGCTGATTCTATAGATGCAGTGATTGCAAAATTCCAACATGCT
    GTTATTCAAAATCAAATGGCAGAATTAATCCATCATTTTAATCAGTATGATTGTGTTGAA
    GAAACCGAATTATTTAACATAGTACAGCAAGTAGTAGCGCATGCCATTAACCCAACACTA
    CCACATGCAAATGAGTTAAAAGATATTTTGTTTGGACCAACAATTACTGTCAAAGCGTTG
    TTAAATATGAGAATGGAAAATAAAGTAAAGCAATATTTAAATATTGAGTTAGATAATCCG
    ATAAAAAAAGAGGTGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1758

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2080 [new locus tag: NWMN_RS12050 ]
  • symbol: NWMN_2080
  • description: hypothetical protein
  • length: 585
  • theoretical pI: 5.99704
  • theoretical MW: 67210.8
  • GRAVY: -0.184957

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    PKinase (CL0016) IucA_IucC; IucA / IucC family (PF04183; HMM-score: 174.9)
    and 1 more
    no clan defined FhuF; Ferric iron reductase FhuF-like transporter (PF06276; HMM-score: 96.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001013
    • TAT(Tat/SPI): 0.000096
    • LIPO(Sec/SPII): 0.000314
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQYHMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLREELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYSESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQFIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPTPFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNMLNQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQKVIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGPHFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAELIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRMENKVKQYLNIELDNPIKKEV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Fur* (repression) regulon, SigB (activation) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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