PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2138 [new locus tag: SAUSA300_RS11775 ]
• pan locus tag^?: SAUPAN005570000
• symbol: SAUSA300_2138
• pan gene symbol^?: sfnaB
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2138 [new locus tag: SAUSA300_RS11775 ]
• symbol: SAUSA300_2138
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2312182..2313939
• length: 1758
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912827 NCBI
• RefSeq: YP_494775 NCBI
• BioCyc: see SAUSA300_RS11775
• MicrobesOnline: 1293653 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGTATATCTTGAATGGGCAAAGGCAGATAGAAATATTCAATATCGTGTAATTAACGCC
  ATTATTAAAGAACGTATTTACCCCGAGCAAACATTTATTTCGCAAAAAGGATCTTTAATT
  GAAATTCAGTATCATATGCATGTGTTGACTATTGAAGTTGTTAGAAAAAGTGCATTAGAA
  CGCTATGAGTTTACAGGTGATATTACTTATTTAAATAAAGGTGAAACGTCATTAATTATA
  ACTTTAGAAGGTTTATTAGATGTGTTGAATCATGACTTTGATATCCCTATTTCAGAGCGA
  CTACGCGAAGAGTTAATACACAGTCGAGATAGTTTAGTTGAAACATATAAGCAAATGTCT
  CACAGACAAACGTTAATAAGTCAAAGTTTTAAATTTTCAAGGTTACCACAAGATATTAAC
  TTTTTTTCATGGTTACAACATGTAAAAGATAGTGATAAGACAGATGATTTAACTTATTCT
  GAGAGTTTGGTACCAGAGGGGCATCCAACACACCCTTTAACCAAAACGAAATTGCCCTTA
  ACTATGGAAGAAGTACGAGCATATGCACCTGAGTTTGAAAAAGAAATCCCTTTGCAAATT
  ATGATGATTGAAAAAGACCATGTTGTGTGCACAGCTATGGATGGTAATGATCAATTTATT
  ATTGATGAAATAATTCCCGAATACTACAATCAGATTCGTGTGTTTTTAAAGAGTTTAGGT
  TTGAAAAGTGAAGACTATAGAGCGATTTTAGTACATCCTTGGCAATATGATCATACGATA
  GGGAAATATTTTGAAGCATGGATTGCTAAAAAAATATTAATTCCAACGCCGTTTACAATA
  CTTTCAAAAGCAACTTTATCATTTAGGACGATGTCTTTAATTGATAAACCATACCATGTT
  AAGTTGCCCGTCGATGCACAAGCAACAAGTGCCGTTAGAACAGTCTCAACTGTGACTACT
  GTAGATGGACCAAAGTTAAGTTATGCTTTACAAAACATGTTGAATCAATATCCAGGATTT
  AAAGTTGCTATGGAACCGTTCGGTGAATATGCAAATGTTGATAAAGATAGGGCACGTCAG
  CTTGCTTGTATTATAAGACAAAAGCCTGAAATTGATGGAAAAGGTGCAACAGTAGTTAGT
  GCAAGTCTAGTTAATAAAAATCCAATAGATCAAAAAGTTATCGTGGATAGTTACTTAGAG
  TGGTTAAATCAAGGAATTACTAAAGAAAGTATTACGACATTTATTGAACGATACGCTCAA
  GCATTAATCCCGCCTTTAATTGCTTTTATTCAAAATTATGGAATTGCTTTAGAAGCACAC
  ATGCAAAATACAGTAGTGAACTTGGGGCCACATTTTGACATTCAATTTTTAGTGAGAGAT
  TTAGGTGGTTCTAGAATTGATTTAGAAACATTACAACATCGTGTATCAGATATTAAAATT
  ACAAATGATAGTTTAATAGCTGATTCTATAGATGCAGTGATTGCAAAATTCCAACATGCT
  GTTATTCAAAATCAAATGGCAGAATTAATCCATCATTTTAATCAGTATGATTGTGTTGAA
  GAAACCGAATTATTTAACATAGTACAGCAAGTAGTAGCGCATGCCATTAACCCAACACTA
  CCACATGCAAATGAGTTAAAAGATATTTTGTTTGGACCAACAATTACTGTCAAAGCGTTG
  TTAAATATGAGAATGGAAAATAAAGTAAAGCAATATTTAAATATTGAGTTAGATAATCCG
  ATAAAAAAAGAGGTGTAG
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  1758
  

⊟Protein

⊟General

• locus tag: SAUSA300_2138 [new locus tag: SAUSA300_RS11775 ]
• symbol: SAUSA300_2138
• description: hypothetical protein
• length: 585
• theoretical pI: 5.99704
• theoretical MW: 67210.8
• GRAVY: -0.184957

⊟Function

• TIGRFAM:
• TheSEED  :
  • Siderophore synthetase superfamily, group B
  • Staphyloferrin A synthase (EC 6.3.2.57)
  Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Uncharacterized siderophore biosynthesis protein near heme transporter HtsABC
• PFAM:
  PKinase (CL0016) IucA_IucC; IucA / IucC family (PF04183; HMM-score: 174.9)
  and 1 more
  no clan defined FhuF; Ferric iron reductase FhuF-like transporter (PF06276; HMM-score: 96.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001013
  • TAT(Tat/SPI): 0.000096
  • LIPO(Sec/SPII): 0.000314
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160712 NCBI
• RefSeq: YP_494775 NCBI
• UniProt: A0A0H2XFQ5 UniProt

⊟Protein sequence

• MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQYHMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLREELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYSESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQFIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPTPFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNMLNQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQKVIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGPHFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAELIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRMENKVKQYLNIELDNPIKKEV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_2137 < SAUSA300_2138 < SAUSA300_2139

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications