PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1981 [new locus tag: SA_RS11415 ]
• pan locus tag^?: SAUPAN005570000
• symbol: SA1981
• pan gene symbol^?: sfnaB
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1981 [new locus tag: SA_RS11415 ]
• symbol: SA1981
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2250420..2252177
• length: 1758
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124897 NCBI
• RefSeq: NP_375292 NCBI
• BioCyc: see SA_RS11415
• MicrobesOnline: 104318 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  
  ATGGTATATCTTGAATGGGCAAAGGCAGATAGAAATATTCAATATCGTGTAATTAACGCC
  ATTATTAAAGAACGTATTTACCCCGAGCAAACATTTATTTCGCAAAAAGGATCTTTAATT
  GAAATTCAGTATCATATGCATGTGTTGACTATTGAAGTTGTTAGAAAAAGTGCATTAGAA
  CGCTATGAGTTTACAGGTGATATTACTTATTTAAATAAAGGTGAAACGTCATTAATTATA
  ACTTTAGAAGGTTTGTTAGATGTGTTGAATCATGACTTTGATATCCCTATTTCAGAGCGA
  CTACGCGAAGAGTTAATACACAGTCGAGATAGTTTAGTTGAAACATATAAGCAAATGTCT
  CACAGACAAACATTAATAAGTCAAAGTTTTAAATTTTCAAGATTACCACAAGATATTAAC
  TTTTTTTCATGGTTACAACATGTAAAAGATAGTGATAAGACAGATGATTTAACTTATTCT
  GAGAGCTTGGTACCAGAGGGGCATCCAACACACCCTTTAACCAAAACGAAATTGCCCTTA
  ACTATGGAAGAAGTACGAGCATATGCACCTGAGTTTGAAAAAGAAATCCCTTTGCAAATT
  ATGATGATTGAAAAAGATCATGTTGTGTGCACAGCTATGGATGGTAATGATCAATTTATT
  ATTGATGAAATAATTCCCGAATACTACAATCAGATTCGTGTGTTTTTAAAGAGTTTAGGT
  TTGAAAAGTGAAGACTATAGAGCGATTTTAGTACATCCTTGGCAATATGATCATACGATA
  GGGAAATATTTTGAAGCATGGATTGCTAAAAAAATATTAATTCCAACGCCGTTTACAATA
  CTTTCAAAAGCAACTTTATCATTTAGGACGATGTCTTTAATTGATAAACCATACCATGTT
  AAGTTGCCCGTCGATGCACAAGCAACAAGTGCCGTTAGAACAGTCTCAACTGTGACTACT
  GTAGATGGACCAAAGTTAAGTTATGCTTTACAAAACATGTTGAATCAATATCCAGGATTT
  AAAGTTGCTATGGAACCGTTCGGTGAATATGCAAATGTTGATAAAGATAGGGCACGTCAG
  CTTGCTTGTATTATAAGACAAAAGCCTGAAATTGATGGAAAAGGTGCAACAGTAGTTAGT
  GCAAGTCTAGTTAATAAAAATCCAATAGATCAAAAAGTTATCGTGGATAGTTACTTAGAG
  TGGTTAAATCAAGGAATTACTAAAGAAAGTATTACGACATTTATTGAACGATACGCTCAA
  GCATTAATCCCGCCTTTAATTGCTTTTATTCAAAATTATGGAATTGCATTAGAAGCACAC
  ATGCAAAATACAGTAGTGAACTTGGGGCCACATTTTGACATTCAATTTTTAGTGAGAGAT
  TTAGGTGGTTCTAGAATTGATTTAGAAACATTACAACATCGTGTATCAGATATTAAAATT
  ACAAATGATAGTTTAATAGCTGATTCTATAGATGCAGTGATTGCAAAATTCCAACATGCT
  GTTATTCAAAATCAAATGGCAGAATTAATCCATCATTTTAATCAGTATGATTGTGTTGAA
  GAAACCGAATTATTTAACATAGTACAGCAAGTAGTAGCGCATGCTATTAACCCAACACTA
  CCACATGCAAATGAGTTAAAAGATATTTTGTTTGGACCAACAATTACTGTCAAAGCGTTG
  TTAAATATGAGAATGGAAAATAAAGTAAAGCAATATTTAAATATTGAGTTAGATAATCCG
  ATAAAAAAAGAGGTGTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1758
  

⊟Protein

⊟General

• locus tag: SA1981 [new locus tag: SA_RS11415 ]
• symbol: SA1981
• description: hypothetical protein
• length: 585
• theoretical pI: 5.99704
• theoretical MW: 67210.8
• GRAVY: -0.184957

⊟Function

• TIGRFAM:
• TheSEED  :
  • Siderophore synthetase superfamily, group B
  • Staphyloferrin A synthase (EC 6.3.2.57)
  Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Uncharacterized siderophore biosynthesis protein near heme transporter HtsABC
• PFAM:
  PKinase (CL0016) IucA_IucC; IucA / IucC family (PF04183; HMM-score: 174.9)
  and 1 more
  no clan defined FhuF; Ferric iron reductase FhuF-like transporter (PF06276; HMM-score: 96.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001013
  • TAT(Tat/SPI): 0.000096
  • LIPO(Sec/SPII): 0.000314
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927759 NCBI
• RefSeq: NP_375292 NCBI
• UniProt: A0A0H3JNG6 UniProt

⊟Protein sequence

• MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQYHMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLREELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYSESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQFIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPTPFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNMLNQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQKVIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGPHFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAELIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRMENKVKQYLNIELDNPIKKEV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1980 < SA1981 < SA1982

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications