PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1845 [new locus tag: NWMN_RS10600 ]
• pan locus tag^?: SAUPAN004936000
• symbol: NWMN_1845
• pan gene symbol^?: hisR
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1845 [new locus tag: NWMN_RS10600 ]
• symbol: NWMN_1845
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2056076..2056378
• length: 303
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332250 NCBI
• RefSeq: YP_001332879 NCBI
• BioCyc:
• MicrobesOnline: 3707433 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  
  ATGCAAATTGAAAAATTACGAGGTGCAGCGTTAGATGAATTGTTTGATGCAATACTAACG
  TTAGAAAATAGAGAAGAATGTTACCAATTTTTCGATGATTTGTGTACTGTAAATGAAATT
  CAATCACTGTCTCAAAGATTACAAGTTGCTAAAATGATTAAGCAAGGTTATACCTATGCA
  ACGATTGAACAAGAATCTGGAGCATCGACTGCAACGATTTCTAGAGTGAAGCGTTCATTA
  CAATGGGGTAATGATGCTTATACAATGATTTTAGATCGTATGAATATTGAAACAAATGAA
  TAA
  60
  120
  180
  240
  300
  303
  

⊟Protein

⊟General

• locus tag: NWMN_1845 [new locus tag: NWMN_RS10600 ]
• symbol: NWMN_1845
• description: hypothetical protein
• length: 100
• theoretical pI: 4.27043
• theoretical MW: 11518.9
• GRAVY: -0.402

⊟Function

• TIGRFAM:
  Unknown function General TrpR homolog YerC/YecD (TIGR02531; HMM-score: 162.2)
  and 2 more
  Metabolism Amino acid biosynthesis Aromatic amino acid family trp operon repressor (TIGR01321; HMM-score: 20.8)
  Signal transduction Regulatory functions DNA interactions trp operon repressor (TIGR01321; HMM-score: 20.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  HTH (CL0123) Trp_repressor; Trp repressor protein (PF01371; HMM-score: 117.4)
  and 4 more
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 29.5)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 19.5)
  HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 14.5)
  HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effector: Histidine
• genes regulated by HisR*, TF important in Histidine biosynthesisRegPrecise

  repression

  NWMN_0423

  NWMN_0813 > NWMN_0814

  hisR*

  hisIE < hisF < hisA < hisH < hisB < hisC < hisD < hisG < hisZ

  transcription units transferred from N315 data RegPrecise

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003872
  • TAT(Tat/SPI): 0.000703
  • LIPO(Sec/SPII): 0.000578
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222057 NCBI
• RefSeq: YP_001332879 NCBI
• UniProt: A0A0H3K9F0 UniProt

⊟Protein sequence

• MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLSQRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: HisR* (repression) regulon

  HisR*

  (TF)

  important in Histidine biosynthesis; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Semen A Leyn, Marat D Kazanov, Natalia V Sernova, Ekaterina O Ermakova, Pavel S Novichkov, Dmitry A Rodionov
Genomic reconstruction of the transcriptional regulatory network in Bacillus subtilis.
J Bacteriol: 2013, 195(11);2463-73
[PubMed:23504016] [WorldCat.org] [DOI] (I p)