PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2575 [new locus tag: NWMN_RS14775 ]
• pan locus tag^?: SAUPAN006432000
• symbol: hisC
• pan gene symbol^?: —
• synonym:
• product: histidinol-phosphate aminotransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2575 [new locus tag: NWMN_RS14775 ]
• symbol: hisC
• product: histidinol-phosphate aminotransferase
• replicon: chromosome
• strand: -
• coordinates: 2842911..2843924
• length: 1014
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331999 NCBI
• RefSeq: YP_001333609 NCBI
• BioCyc:
• MicrobesOnline: 3708177 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGATTTATATTGATAAAAATGAAAGTCCAGTTACGCCGTTGGATGAAAAAACAATGACG
  TCTATTATTAGTGCAACGCCATATAATTTATATCCTGATGCAGCATATGAACAATTCAAG
  GAAGCTTATGCTAAGTTTTACGGATTATCGCCTGAACAAATTATTGCAGGAAATGGATCT
  GATGAATTGATTCAAAAGTTAATGCTGATCATGCCAGAAGGTCCGGCATTAACGCTAAAT
  CCTGATTTTTTTATGTATCAAGCATATGCGGCACAAGTAAATCGTGAAATTGCATTTGTA
  GATGCAGGATCAGATTTAACGTTTGATTTGGAAACCATTTTAACGAAAATCGATGAAGTA
  CAACCATCATTTTTTATTATGAGTAATCCACATAACCCTTCAGGCAAGCAATTTGATACG
  GCATTTTTAACAGCTATTGCAGATAAGATGAAAGCATTAAACGGATACTTTGTCATTGAT
  GAAGCATATTTAGATTATGGTACGGCATATGACGTGGAACTGGCACCACACATCTTAAGA
  ATGCGTACATTATCAAAGGCGTTTGGAATTGCCGGCTTAAGATTAGGTGTCTTAATTAGT
  ACTGCTGGAACGATAAAGCATATTCAAAAAATAGAACATCCATATCCATTAAATGTATTT
  ACGCTAAATATTGCGACTTATATTTTTAGACATAGAGAAGAGACAAGACAATTTTTAACG
  ATGCAACGACAGTTAGCTGAGCAGTTAAAACAAATATTTGATACACATGTTGCAGATAAA
  ATGTCAGTGTTCCCATCAAATGCTAATTTTGTACTTACTAAAGGCTCAGCAGCGCAACAA
  TTAGGACAATACGTATATGAACAAGGATTTAAACCTCGCTTTTATGATGAGCCGGTGATG
  AAGGGCTATGTAAGATACTCAATTGCAACAGCATCACAGTTAAAGCAATTAGAAGAAATT
  GTTAAAGAATGGAGTGCAAAATATGATTTATCAAAAACAACGAAACACAGCTGA
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⊟Protein

⊟General

• locus tag: NWMN_2575 [new locus tag: NWMN_RS14775 ]
• symbol: HisC
• description: histidinol-phosphate aminotransferase
• length: 337
• theoretical pI: 5.65809
• theoretical MW: 38240.5
• GRAVY: -0.201187

⊟Function

• TIGRFAM:
  Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 198.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 163.9)
  and 8 more
  Cellular processes Cellular processes Biosynthesis of natural products capreomycidine synthase (TIGR03947; HMM-score: 46)
  tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 31.9)
  putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 27.7)
  Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 26.7)
  beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 19.9)
  succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 18.3)
  succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 17.9)
  LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 15)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 126.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: pyridoxal 5'-phosphate
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.0182
  • TAT(Tat/SPI): 0.000757
  • LIPO(Sec/SPII): 0.00112
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222787 NCBI
• RefSeq: YP_001333609 NCBI
• UniProt: A0A0H3KAX4 UniProt

⊟Protein sequence

• MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYAKFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDAGSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVIDEAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLNVFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGSAAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTTKHS

⊟Experimental data

• experimentally validated: data available for COL
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hisIE < hisF < hisA < hisH < hisB < hisC < hisD < hisG < hisZ

⊟Regulation

• regulators: HisR* (repression) regulon, CodY (repression) regulon

  HisR*	(TF)	important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications