PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0999 [new locus tag: NWMN_RS05625 ]
• pan locus tag^?: SAUPAN001671000
• symbol: NWMN_0999
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0999 [new locus tag: NWMN_RS05625 ]
• symbol: NWMN_0999
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1105554..1106327
• length: 774
• essential: unknown

⊟Accession numbers

• Gene ID: 5330603 NCBI
• RefSeq: YP_001332033 NCBI
• BioCyc:
• MicrobesOnline: 3706550 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAGTGAGGAACAAGACATTTTACAAGAACTAGGTATTGAAGAAATTAACGAAGATACT
  CAGAACTATTATTCAATTATGGTATATGGCAAATCAGGAACCGGAAAGACGACTTTAGCC
  ACTAGAGAAAACAACGCTTTTATTATTGATATTCACGAAGATGGCACTCAAGTAACGCGG
  CAAGGTTTTGTGAAGAGGGTCGACAATTACATTGCTTTTAGAAACACAATTGCGAGTATT
  GAATCGATTGTAAATACAGCTAGACAAAGAGGAAAGTTACTTGATGTGGTTGTAATTGAA
  ACAGCACAAAAGTTAAGAGATATAACGCTGACTCATGTGATGAACACGCACCAAGTCAAA
  AAAGCAAGAATTCAAGATTATGGGGAAACATCTAAATTAATTGTTAACTCGATTAGGCAC
  CTATTAAAGGTTAAAGATAAGCTCGGATTTCACGTTGTGCTTACAGGACATGAAGGGCTT
  AACTCAGAAGATAAAGATGAGAACGGAAAAATTATTAACCCTAGAATATCAATTGAAGTA
  CAACCGGCAATACACAACAACTTAGTAACTCAGTTCGACATTATAGGACACACATTTATA
  GAAGATCATACAGATGAGAACGGAAATGCGACACACGATTATGTGTTTTCTGTAGAACCT
  TCTAATTTATATACAACTAAAGTTAGGCATAATCCGCAAATAACAATCAATAATCCAGGT
  ATTAAAAATGCTTCAATTTCAAAAATTATAGATATGGCACAAAACGGAAACTAA
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⊟Protein

⊟General

• locus tag: NWMN_0999 [new locus tag: NWMN_RS05625 ]
• symbol: NWMN_0999
• description: hypothetical protein
• length: 257
• theoretical pI: 6.2869
• theoretical MW: 28960.3
• GRAVY: -0.477043

⊟Function

• TIGRFAM:
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage nucleotide-binding protein (TIGR01618; HMM-score: 37.8)
  and 1 more
  surface protein repeat SSSPR-51 (TIGR04308; HMM-score: 19.2)
• TheSEED:
• PFAM:
  P-loop_NTPase (CL0023) AAA_24; AAA domain (PF13479; HMM-score: 85.6)
  and 14 more
  RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 20)
  AAA_22; AAA domain (PF13401; HMM-score: 19.1)
  PEP-carboxyk (CL0374) PEPCK_ATP; Phosphoenolpyruvate carboxykinase (PF01293; HMM-score: 17.9)
  P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 16.6)
  AAA_25; AAA domain (PF13481; HMM-score: 14.5)
  AAA_33; AAA domain (PF13671; HMM-score: 14.3)
  AAA_18; AAA domain (PF13238; HMM-score: 14.2)
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 13.9)
  NB-ARC; NB-ARC domain (PF00931; HMM-score: 13.7)
  RNA_helicase; RNA helicase (PF00910; HMM-score: 13.5)
  Sigma54_activat; Sigma-54 interaction domain (PF00158; HMM-score: 13.3)
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 12.3)
  DUF2075; Uncharacterized conserved protein (DUF2075) (PF09848; HMM-score: 10.6)
  CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 10)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006969
  • TAT(Tat/SPI): 0.001034
  • LIPO(Sec/SPII): 0.001184
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221211 NCBI
• RefSeq: YP_001332033 NCBI
• UniProt: A0A0H3K848 UniProt

⊟Protein sequence

• MSEEQDILQELGIEEINEDTQNYYSIMVYGKSGTGKTTLATRENNAFIIDIHEDGTQVTRQGFVKRVDNYIAFRNTIASIESIVNTARQRGKLLDVVVIETAQKLRDITLTHVMNTHQVKKARIQDYGETSKLIVNSIRHLLKVKDKLGFHVVLTGHEGLNSEDKDENGKIINPRISIEVQPAIHNNLVTQFDIIGHTFIEDHTDENGNATHDYVFSVEPSNLYTTKVRHNPQITINNPGIKNASISKIIDMAQNGN

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0996 > NWMN_0997 > NWMN_0998 > NWMN_0999 > NWMN_1000 > NWMN_1001

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications