PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0998 [new locus tag: NWMN_RS05620 ]
• pan locus tag^?: SAUPAN001670000
• symbol: NWMN_0998
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0998 [new locus tag: NWMN_RS05620 ]
• symbol: NWMN_0998
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1105075..1105554
• length: 480
• essential: unknown

⊟Accession numbers

• Gene ID: 5330602 NCBI
• RefSeq: YP_001332032 NCBI
• BioCyc:
• MicrobesOnline: 3706549 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGCTAATCTATATGAGCTATCAGAAGCATTTAAAGAGATGTCTAATCAAGATGAATTA
  GATCCAACATTACTAAAAGATACATTAGATTCTATCAAAGCAGAAATGAACGTCAAAGTA
  GATAACATTGTCAATTGGAGACGTGAAACTTTAGGTGACATAGATGTCATAGATAAAGAA
  ATTAAGAGACTTCAAAATTTAAAAAAACAAAAACAAAATTTAACTGATCGTTTAAGAGAT
  TACTTAAAAGAGATGTTAGAAACACAGGAAGTAGATAGTTACCGCACAGCTACTAATCAT
  ATTTACAAGCGCAAAAACGGGGCTAGTAAAAATATTATCGATGAAAAACTTATTCCAAAG
  GATTATTGGCTATCACAAGCGCCAAAGCTTAATTCTAAGCAACTAATCGATGATTTGAAA
  GCTGGCAAAGATATTCCGGGCGCTGAATTAAAGGTAACGGAAAGTTTGGTGATTAAGTGA
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⊟Protein

⊟General

• locus tag: NWMN_0998 [new locus tag: NWMN_RS05620 ]
• symbol: NWMN_0998
• description: hypothetical protein
• length: 159
• theoretical pI: 6.04495
• theoretical MW: 18478
• GRAVY: -0.787421

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Pathogenesis phospholipase C, phosphocholine-specific (TIGR03396; EC 3.1.4.3; HMM-score: 10.4)
• TheSEED:
• PFAM:
  no clan defined Sipho_Gp157; Siphovirus Gp157 (PF05565; HMM-score: 120.6)
  and 11 more
  NICE-3; NICE-3 protein (PF07406; HMM-score: 18.6)
  HAUS-augmin3; HAUS augmin-like complex subunit 3 (PF14932; HMM-score: 17.6)
  Cystatin (CL0121) YPEB; YpeB sporulation (PF14620; HMM-score: 15.3)
  TPR (CL0020) ParcG; Parkin co-regulated protein (PF10274; HMM-score: 14.2)
  no clan defined DNA_repr_REX1B; DNA repair REX1-B (PF14966; HMM-score: 13.9)
  HTH (CL0123) DUF2316; Uncharacterized protein conserved in bacteria (DUF2316) (PF10078; HMM-score: 13.1)
  no clan defined EcoEI_R_C; EcoEI R protein C-terminal (PF08463; HMM-score: 12.9)
  Osmo_CC; Osmosensory transporter coiled coil (PF08946; HMM-score: 12.6)
  Phage_Mu_Gam; Bacteriophage Mu Gam like protein (PF07352; HMM-score: 12.5)
  S15_NS1 (CL0600) WHEP-TRS; WHEP-TRS domain (PF00458; HMM-score: 11.5)
  no clan defined Fib_alpha; Fibrinogen alpha/beta chain family (PF08702; HMM-score: 10.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000792
  • TAT(Tat/SPI): 0.000098
  • LIPO(Sec/SPII): 0.000224
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221210 NCBI
• RefSeq: YP_001332032 NCBI
• UniProt: A0A0H3K7G6 UniProt

⊟Protein sequence

• MANLYELSEAFKEMSNQDELDPTLLKDTLDSIKAEMNVKVDNIVNWRRETLGDIDVIDKEIKRLQNLKKQKQNLTDRLRDYLKEMLETQEVDSYRTATNHIYKRKNGASKNIIDEKLIPKDYWLSQAPKLNSKQLIDDLKAGKDIPGAELKVTESLVIK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0996 > NWMN_0997 > NWMN_0998 > NWMN_0999 > NWMN_1000 > NWMN_1001

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications