PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0192 [new locus tag: NWMN_RS01075 ]
• pan locus tag^?: SAUPAN001136000
• symbol: NWMN_0192
• pan gene symbol^?: tarS
• synonym:
• product: glycosyl transferase group 2 family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0192 [new locus tag: NWMN_RS01075 ]
• symbol: NWMN_0192
• product: glycosyl transferase group 2 family protein
• replicon: chromosome
• strand: +
• coordinates: 248706..250427
• length: 1722
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330046 NCBI
• RefSeq: YP_001331227 NCBI
• BioCyc:
• MicrobesOnline: 3705723 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAATTTTCAGTAATAGTTCCAACATACAATTCAGAAAAGTATATAACAGAATTACTT
  AATAGCCTTGCGAAACAAGATTTTCCGAAAACTGAATTTGAAGTGGTTGTAGTTGATGAC
  TGTTCAACAGATCAAACGTTACAAATAGTTGAAAAGTATCGCAATAAATTGAACTTGAAA
  GTAAGTCAACTCGAAACAAATTCTGGTGGTCCAGGTAAACCTAGAAATGTGGCGTTAAAA
  CAAGCAGAAGGTGAATTTGTATTATTTGTGGACTCCGATGACTATATAAACAAAGAGACT
  TTAAAGGATGCAGCAGCATTTATTGATGAACATCACTCAGATGTCTTATTGATTAAAATG
  AAAGGTGTTAATGGTCGTGGTGTACCACAATCTATGTTTAAAGAAACAGCACCTGAAGTT
  ACTTTGTTAAATTCAAGAATTATCTATACTTTAAGCCCGACTAAAATCTATAGAACAGCA
  TTACTAAAAGATAATGACATTTATTTTCCAGAAGAATTAAAGAGTGCAGAAGATCAATTA
  TTTACAATGAAAGCATATTTAAATGCAAATCGAATCAGTGTGTTAAGTGATAAAGCGTAT
  TATTATGCTACAAAGCGTGAAGGTGAACATATGAGTAGTGCGTATGTTTCACCTGAAGAC
  TTTTATGAAGTCATGAGATTGATTGCTGTAGAAATATTAAATACAGATTTAGAAGAAGCC
  CATAAAAATCAAATCTTAGCAGAATTTTTAAATCGTCATTTTAGTTTTTCTCGTACGAAT
  GGCTTCTCACTTAAAGTTAAACTAGAAGATCAACCACAATGGATTAATGCTCTAGGAGAC
  TTTATACAAGCAGTTCCAGAACGTGTAGATGCATTGGTGATGAGTAAATTACGACCATTG
  TTGCACTACGCGAGAGCGAAAGATATAGACAACTATAGAACTGTGGAAGAAAGTTACCGT
  CAAGGTCAATACTACCGTTTTGATATTGTAGATGGTAAATTAAACATTCAATTCAATGAA
  GGCGAACCATACTTTAAAGGCATTGATATCGCTAAGCCAAAAGTGAAAATGACAGCATTT
  AAATTTGATAATCATAAAATTGTTACAGAGCTAACGTTAAATGAATTTATGATTGGCGAA
  GGACATTATGATGTCAGACTTAAATTACATTCACGAAACAAGAAGCACACAATGTATGTA
  CCTTTAAGTGTCAATGCGAATAAACAATATCGTTTTAACATTATGTTAGAAGATATTAAA
  GCGTATTTACCTAAAGAAAAAATTTGGGATGTTTTCTTAGAAGTCCAAATAGGTACGGAA
  GTATTTGAAGTGCGTGTTGGTAATCAACGTAATAAATATGCATATACTGCAGAAACAAGT
  GCATTAATTCATTTGAATAATGATTTTTATAGATTAACACCGTATTTCACAAAAGACTTT
  AATAACATTTCGTTATACTTTACAGCTATTACATTAACGGATTCAATCTCATTGAAGTTA
  AAAGGTAAAAACAAAATCATTTTAACTGGTCTGGATCGTGGTTATGTATTTGAAGAAGGT
  ATGGCTAGTGTCGTACTAAAAGACGACATGGTGATGGGAATGTTAAGCCAAACATCAGAA
  AACGAAGTGCAAATCTTACTTAGCAAAGATATTAAAAAGCGAGACTTCAAAAATATTGTT
  AAGTTAAACACTGCACATATCACTTATCCACTAAATAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_0192 [new locus tag: NWMN_RS01075 ]
• symbol: NWMN_0192
• description: glycosyl transferase group 2 family protein
• length: 573
• theoretical pI: 7.17929
• theoretical MW: 66212.4
• GRAVY: -0.380105

⊟Function

• TIGRFAM:
  putative glycosyltransferase, exosortase G-associated (TIGR03111; HMM-score: 57.5)
  mycofactocin system glycosyltransferase (TIGR03965; HMM-score: 56.6)
  Unknown function Enzymes of unknown specificity transferase 2, rSAM/selenodomain-associated (TIGR04283; EC 2.-.-.-; HMM-score: 50.8)
  poly-beta-1,6 N-acetyl-D-glucosamine synthase (TIGR03937; EC 2.4.1.-; HMM-score: 47.8)
  and 8 more
  chitooligosaccharide synthase NodC (TIGR04242; EC 2.4.1.-; HMM-score: 44.2)
  glycosyltransferase domain (TIGR04440; EC 2.-.-.-; HMM-score: 40.9)
  colanic acid biosynthesis glycosyltransferase WcaA (TIGR04017; EC 2.4.-.-; HMM-score: 36.6)
  hopene-associated glycosyltransferase HpnB (TIGR03469; HMM-score: 27.9)
  colanic acid biosynthesis glycosyltransferase WcaE (TIGR04009; EC 2.4.-.-; HMM-score: 27)
  glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 22.2)
  cellulose synthase catalytic subunit (UDP-forming) (TIGR03030; EC 2.4.1.12; HMM-score: 21.7)
  hopanoid biosynthesis associated glycosyl transferase protein HpnI (TIGR03472; HMM-score: 10.5)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  GT-A (CL0110) Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 112)
  and 6 more
  Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 57.7)
  Glyco_tranf_2_2; Glycosyltransferase like family 2 (PF10111; HMM-score: 48.5)
  Glyco_tranf_2_4; Glycosyl transferase family 2 (PF13704; HMM-score: 24.9)
  Glyco_transf_49; Glycosyl-transferase for dystroglycan (PF13896; HMM-score: 14.5)
  DnaA_N (CL0494) DNA_pol3_a_NII; DNA polymerase III polC-type N-terminus II (PF11490; HMM-score: 12.9)
  no clan defined DUF4478; Domain of unknown function (DUF4478) (PF14793; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00796
  • TAT(Tat/SPI): 0.000139
  • LIPO(Sec/SPII): 0.001355
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220404 NCBI
• RefSeq: YP_001331227 NCBI
• UniProt: A0A0H3KA99 UniProt

⊟Protein sequence

• MKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCSTDQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETLKDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRTALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVSPEDFYEVMRLIAVEILNTDLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWINALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGKLNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHSRNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQRNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKIILTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTAHITYPLNK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ispD > NWMN_0190 > NWMN_0191 > NWMN_0192

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Stephanie Brown, Guoqing Xia, Lyly G Luhachack, Jennifer Campbell, Timothy C Meredith, Calvin Chen, Volker Winstel, Cordula Gekeler, Javier E Irazoqui, Andreas Peschel, Suzanne Walker
Methicillin resistance in Staphylococcus aureus requires glycosylated wall teichoic acids.
Proc Natl Acad Sci U S A: 2012, 109(46);18909-14
[PubMed:23027967] [WorldCat.org] [DOI] (I p)