PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000198
• pan locus tag^?: SAUPAN001136000
• symbol: tarS
• pan gene symbol^?: tarS
• synonym:
• product: poly(ribitol-phosphate) beta-N-acetylglucosaminyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000198
• symbol: tarS
• product: poly(ribitol-phosphate) beta-N-acetylglucosaminyltransferase
• replicon: chromosome
• strand: +
• coordinates: 238485..240206
• length: 1722
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  
  ATGATGAAATTTTCAGTAATAGTTCCAACATACAATTCAGAAAAGTATATAACAGAATTA
  CTTAATAGCCTTGCGAAACAAGATTTTCCGAAAACTGAATTTGAAGTGGTTGTAGTTGAT
  GACTGTTCAACAGATCAAACGTTACAAATAGTTGAAAAGTATCGCAATAAATTGAACTTG
  AAAGTAAGTCAACTCGAAACAAATTCTGGTGGTCCAGGTAAACCTAGAAATGTGGCGTTA
  AAACAAGCAGAAGGTGAATTTGTATTATTTGTGGACTCCGATGACTATATAAACAAAGAG
  ACTTTAAAAGATGCAGCAGCATTTATTGATGAACATCACTCCGATGTCTTATTGATTAAA
  ATGAAAGGTGTTAATGGTCGTGGTGTACCACAATCTATGTTTAAAGAAACAGCACCTGAA
  GTTACTTTGTTAAATTCAAGAATTATCTATACTTTAAGCCCGACTAAAATCTATAGAACA
  ACATTACTAAAAGATAATGACATTTATTTTCCAGAAGAATTAAAGAGTGCAGAAGATCAA
  TTATTTACAATAAAAGCATATTTGAATGCAAATCGAATCAGTGTGTTAAGTGATAAAGCG
  TATTATTATGCTACAAAGCGTGAAGGTGAACATATGAGTAGTGCGTATGTTTCACCTGAA
  GACTTTTACGAAGTCATGAGATTGATTGCTGTAGAAATATTAAATGCAGATTTAGAAGAA
  GCTCATAAAGATCAAATCTTAGCAGAATTTTTAAATCGTCATTTTAGTTTTTCTCGTACG
  AATGGCTTCTCACTTAAAGTTAAACTAGAAGATCAACCGCAATGGATTAATGCTCTAGGA
  GACTTTATACAAGCAGTTCCAGAACGTGTAGATGCATTGGTGATGAGTAAATTACGACCA
  TTGTTGCACTACGCGAGAGCGAAAGATATAGACAACTATAGAACTGTAGAAGAAAGTTAC
  CGTCAAGGTCAATACTACCGTTTTGATATTGTAGATGGTAAATTAAACATTCAATTCAAT
  GAAGGCGAACCATACTTTGAAGGCATTGATATCGCTAAGCCAAAAGTGAAAATGACAGCA
  TTTAAATTTGATAATCATAAAATTGTTACAGAGCTAACGTTAAATGAATTTATGATTGGC
  GAAGGACATTATGATGTCAGACTTAAATTACATTCACGAAACAAGAAGCACACAATGTAT
  GTACCTTTAAGTGTCAATGCGAATAAACAATATCGTTTTAACATTATGTTAGAAGATATT
  AAAGCGTATTTACCTAAAGAAAAAATTTGGGATGTTTTCTTAGAAGTCCAAATAGGTACG
  GAAGTATTTGAAGTGCGTGTTGGTAATCAACGTAATAAATATGCATATACTGCAGAAACA
  AGTGCATTAATTCATTTGAATAATGATTTTTATAGATTAACACCGTATTTCACAAAAGAC
  TTTAATAACATTTCGTTATACTTTACAGCTATTACATTAACGGATTCAATCTCAATGAAG
  TTAAAAGGTAAGAACAAAATCATTTTAACTGGTCTGGATCGTGGTTATGTATTTGAAGAA
  GGTATGGCTAGTGTCGTGCTAAAAGACGACATGATTGTGGGAATGTTAAGCCAAACGTCA
  GAAAACGAAGTGGAAATCTTACTTAGTAAAGATATTAAGAAGCGAGACTTCAAAAATATT
  GTTAAGTTAAACACTGCACATATGACTTACTCGCTAAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1722
  

⊟Protein

⊟General

• locus tag: JSNZ_000198
• symbol: TarS
• description: poly(ribitol-phosphate) beta-N-acetylglucosaminyltransferase
• length: 573
• theoretical pI: 6.53178
• theoretical MW: 66222.3
• GRAVY: -0.367365

⊟Function

• reaction:
  EC 2.4.1.355?  ExPASy
  non-specified enzyme name
• TIGRFAM:
  mycofactocin system glycosyltransferase (TIGR03965; HMM-score: 56.8)
  putative glycosyltransferase, exosortase G-associated (TIGR03111; HMM-score: 56.7)
  Unknown function Enzymes of unknown specificity transferase 2, rSAM/selenodomain-associated (TIGR04283; EC 2.-.-.-; HMM-score: 50.9)
  poly-beta-1,6 N-acetyl-D-glucosamine synthase (TIGR03937; EC 2.4.1.-; HMM-score: 48.2)
  and 8 more
  chitooligosaccharide synthase NodC (TIGR04242; EC 2.4.1.-; HMM-score: 44.4)
  glycosyltransferase domain (TIGR04440; EC 2.-.-.-; HMM-score: 40.6)
  colanic acid biosynthesis glycosyltransferase WcaA (TIGR04017; EC 2.4.-.-; HMM-score: 36.6)
  hopene-associated glycosyltransferase HpnB (TIGR03469; HMM-score: 28.1)
  colanic acid biosynthesis glycosyltransferase WcaE (TIGR04009; EC 2.4.-.-; HMM-score: 27.6)
  glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 22.2)
  cellulose synthase catalytic subunit (UDP-forming) (TIGR03030; EC 2.4.1.12; HMM-score: 21.7)
  hopanoid biosynthesis associated glycosyl transferase protein HpnI (TIGR03472; HMM-score: 10.7)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  E-set (CL0159) TarS_C2; TarS C-terminal domain 2 (PF22377; HMM-score: 169.4)
  and 8 more
  TarS_C1; TarS beta-glycosyltransferase C-terminal domain 1 (PF18674; HMM-score: 132.9)
  GT-A (CL0110) Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 113.3)
  Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 51)
  Glyco_tranf_2_2; Glycosyltransferase like family 2 (PF10111; HMM-score: 48.9)
  no clan defined TarS_linker; Glycosyl transferase TarS linker domain (PF22181; HMM-score: 45.9)
  GT-A (CL0110) Glyco_tranf_2_4; Glycosyl transferase family 2 (PF13704; HMM-score: 19.5)
  no clan defined DUF4478; Pyrimidine/purine nucleotide 5'-monophosphate nucleosidases (PF14793; HMM-score: 14.6)
  GT-A (CL0110) Glyco_transf_49; Glycosyl-transferase for dystroglycan (PF13896; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.7728
  • Cytoplasmic Membrane Score: 0.1728
  • Cell wall & surface Score: 0.0197
  • Extracellular Score: 0.0347
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007451
  • TAT(Tat/SPI): 0.000169
  • LIPO(Sec/SPII): 0.001186
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCSTDQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETLKDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRTTLLKDNDIYFPEELKSAEDQLFTIKAYLNANRISVLSDKAYYYATKREGEHMSSAYVSPEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWINALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGKLNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHSRNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQRNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISMKLKGKNKIILTGLDRGYVFEEGMASVVLKDDMIVGMLSQTSENEVEILLSKDIKKRDFKNIVKLNTAHMTYSLK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Stephanie Brown, Guoqing Xia, Lyly G Luhachack, Jennifer Campbell, Timothy C Meredith, Calvin Chen, Volker Winstel, Cordula Gekeler, Javier E Irazoqui, Andreas Peschel, Suzanne Walker
Methicillin resistance in Staphylococcus aureus requires glycosylated wall teichoic acids.
Proc Natl Acad Sci U S A: 2012, 109(46);18909-14
[PubMed:23027967] [WorldCat.org] [DOI] (I p)