PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0252 [new locus tag: SAUSA300_RS01345 ]
• pan locus tag^?: SAUPAN001136000
• symbol: SAUSA300_0252
• pan gene symbol^?: tarS
• synonym:
• product: glycosyl transferase, group 2 family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0252 [new locus tag: SAUSA300_RS01345 ]
• symbol: SAUSA300_0252
• product: glycosyl transferase, group 2 family protein
• replicon: chromosome
• strand: +
• coordinates: 303316..305040
• length: 1725
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912852 NCBI
• RefSeq: YP_492966 NCBI
• BioCyc: see SAUSA300_RS01345
• MicrobesOnline: 1291767 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATGAAATTTTCAGTAATAGTTCCAACATACAATTCAGAAAAGTATATAACAGAATTA
  CTTAATAGCCTTGCGAAACAAGATTTTCCGAAAACTGAATTTGAAGTGGTTGTAGTTGAT
  GACTGTTCAACAGATCAAACGTTACAAATAGTTGAAAAGTATCGCAATAAATTGAACTTG
  AAAGTAAGTCAACTCGAAACAAATTCTGGTGGTCCAGGTAAACCTAGAAATGTGGCGTTA
  AAACAAGCAGAAGGTGAATTTGTATTATTTGTGGACTCCGATGACTATATAAACAAAGAG
  ACTTTAAAGGATGCAGCAGCATTTATTGATGAACATCACTCAGATGTCTTATTGATTAAA
  ATGAAAGGTGTTAATGGTCGTGGTGTACCACAATCTATGTTTAAAGAAACAGCACCTGAA
  GTTACTTTGTTAAATTCAAGAATTATCTATACTTTAAGCCCGACTAAAATCTATAGAACA
  GCATTACTAAAAGATAATGACATTTATTTTCCAGAAGAATTAAAGAGTGCAGAAGATCAA
  TTATTTACAATGAAAGCATATTTAAATGCAAATCGAATCAGTGTGTTAAGTGATAAAGCG
  TATTATTATGCTACAAAGCGTGAAGGTGAACATATGAGTAGTGCGTATGTTTCACCTGAA
  GACTTTTATGAAGTCATGAGATTGATTGCTGTAGAAATATTAAATGCAGATTTAGAAGAA
  GCCCATAAAAATCAAATCTTAGCAGAATTTTTAAATCGTCATTTTAGTTTTTCTCGTACG
  AATGGCTTCTCACTTAAAGTTAAACTAGAAGATCAACCACAATGGATTAATGCTCTAGGA
  GACTTTATACAAGCAGTTCCAGAACGTGTAGATGCATTGGTGATGAGTAAATTACGACCA
  TTGTTGCACTACGCGAGAGCGAAAGATATAGACAACTATAGAACTGTGGAGGAAAGTTAC
  CGTCAAGGTCAATACTACCGTTTTGATATTGTAGATGGTAAATTAAACATTCAATTCAAT
  GAAGGCGAACCATACTTTAAAGGCATTGATATCGCTAAGCCAAAAGTGAAAATGACAGCA
  TTTAAATTTGATAATCATAAAATTGTTACAGAGCTAACGTTAAATGAATTTATGATTGGC
  GAAGGACATTATGATGTCAGACTTAAATTACATTCACGAAACAAGAAGCACACAATGTAT
  GTACCTTTAAGTGTCAATGCGAATAAACAATATCGTTTTAACATTATGTTAGAAGATATT
  AAAGCGTATTTACCTAAAGAAAAAATTTGGGATGTTTTCTTAGAAGTCCAAATAGGTACG
  GAAGTATTTGAAGTGCGTGTTGGTAATCAACGTAATAAATATGCATATACTGCAGAAACA
  AGTGCATTAATTCATTTGAATAATGATTTTTATAGATTAACACCGTATTTCACAAAAGAC
  TTTAATAACATTTCGTTATACTTTACAGCTATTACATTAACGGATTCAATCTCATTGAAG
  TTAAAAGGTAAAAACAAAATCATTTTAACTGGTCTGGATCGTGGTTATGTATTTGAAGAA
  GGTATGGCTAGTGTCGTACTAAAAGACGACATGGTGATGGGAATGTTAAGCCAAACATCA
  GAAAACGAAGTGCAAATCTTACTTAGCAAAGATATTAAAAAGCGAGACTTCAAAAATATT
  GTTAAGTTAAACACTGCACATATCACTTATCCACTAAATAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0252 [new locus tag: SAUSA300_RS01345 ]
• symbol: SAUSA300_0252
• description: glycosyl transferase, group 2 family protein
• length: 574
• theoretical pI: 7.17929
• theoretical MW: 66313.5
• GRAVY: -0.371777

⊟Function

• TIGRFAM:
  putative glycosyltransferase, exosortase G-associated (TIGR03111; HMM-score: 57.6)
  mycofactocin system glycosyltransferase (TIGR03965; HMM-score: 56.9)
  Unknown function Enzymes of unknown specificity transferase 2, rSAM/selenodomain-associated (TIGR04283; EC 2.-.-.-; HMM-score: 50.8)
  poly-beta-1,6 N-acetyl-D-glucosamine synthase (TIGR03937; EC 2.4.1.-; HMM-score: 48.2)
  and 8 more
  chitooligosaccharide synthase NodC (TIGR04242; EC 2.4.1.-; HMM-score: 44.4)
  glycosyltransferase domain (TIGR04440; EC 2.-.-.-; HMM-score: 40.9)
  colanic acid biosynthesis glycosyltransferase WcaA (TIGR04017; EC 2.4.-.-; HMM-score: 36.7)
  hopene-associated glycosyltransferase HpnB (TIGR03469; HMM-score: 27.9)
  colanic acid biosynthesis glycosyltransferase WcaE (TIGR04009; EC 2.4.-.-; HMM-score: 27.3)
  glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 22.2)
  cellulose synthase catalytic subunit (UDP-forming) (TIGR03030; EC 2.4.1.12; HMM-score: 21.7)
  hopanoid biosynthesis associated glycosyl transferase protein HpnI (TIGR03472; HMM-score: 10.7)
• TheSEED  :
  • Glycosyl transferase family protein, putative
• PFAM:
  GT-A (CL0110) Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 112)
  and 6 more
  Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 57.7)
  Glyco_tranf_2_2; Glycosyltransferase like family 2 (PF10111; HMM-score: 48.4)
  Glyco_tranf_2_4; Glycosyl transferase family 2 (PF13704; HMM-score: 24.9)
  Glyco_transf_49; Glycosyl-transferase for dystroglycan (PF13896; HMM-score: 14.6)
  DnaA_N (CL0494) DNA_pol3_a_NII; DNA polymerase III polC-type N-terminus II (PF11490; HMM-score: 12.9)
  no clan defined DUF4478; Domain of unknown function (DUF4478) (PF14793; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007451
  • TAT(Tat/SPI): 0.000169
  • LIPO(Sec/SPII): 0.001186
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160407 NCBI
• RefSeq: YP_492966 NCBI
• UniProt: A0A0H2XF10 UniProt

⊟Protein sequence

• MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCSTDQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETLKDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRTALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVSPEDFYEVMRLIAVEILNADLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWINALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGKLNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHSRNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQRNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKIILTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTAHITYPLNK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ispD > SAUSA300_0250 > SAUSA300_0251 > SAUSA300_0252

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Stephanie Brown, Guoqing Xia, Lyly G Luhachack, Jennifer Campbell, Timothy C Meredith, Calvin Chen, Volker Winstel, Cordula Gekeler, Javier E Irazoqui, Andreas Peschel, Suzanne Walker
Methicillin resistance in Staphylococcus aureus requires glycosylated wall teichoic acids.
Proc Natl Acad Sci U S A: 2012, 109(46);18909-14
[PubMed:23027967] [WorldCat.org] [DOI] (I p)